A comparison of the effects of drought on proline accumulation and peroxidases activity in leaves of Festuca rubra L. and Lolium perenne L.

• Autorzy: Bandurska H , Jozwiak W.
• Języki publikacji: EN

Abstrakty

EN

The effect of soil drought on leaf water content, proline content, and the activity of guaiacol (GuPX) and ascorbate (APX) peroxidases as well as the level of lipid peroxidation were investigated in leaves of drought resistant red fescue (Festuca rubra) and drought sensitive perennial ryegrass (Lolium perenne). Plants were grown under glasshouse conditions in soil pot culture. 26 day-old grasses were exposed to drought by withholding irrigation for 18 days. Water content in leaves of perennial ryegrass decreased more than in red fescue throughout the experimental period. On the other hand, proline content (PC) was higher in red fescue. The activity of APX and GuPX increased in leaves of red fescue, while it did not change in perennial ryegrass. Our data demonstrate that both red fescue and perennial ryegrass were able to survive applied drought, as shown by a lack of stress-induced lipid peroxidation and hence no evidence of oxidative damage. We speculate, that the observed drought stress tolerance at cellular level was associated with the ability to accumulate proline, and to maintain high activity of APX and GuPX, resulting in protection against oxidative damage and lipid peroxidation. It seems that this mechanism works better in red fescue.

Słowa kluczowe

EN

red fescue; grass; drought; proline accumulation; peroxidase; enzyme activity; leaf; Festuca rubra; Lolium perenne; lipid peroxidation; rye-grass

• Wydawca: -
• Czasopismo: Acta Societatis Botanicorum Poloniae
• Rocznik: 2010
• Tom: 79
• Numer: 2
• Opis fizyczny: p.111-116,fig.,ref.

Twórcy

autor

Bandurska H

• Poznan University of Life Sciences, Wolynska 35, 60-637 Poznan, Poland

autor

Jozwiak W.

Bibliografia

• AMAKO K., CHEN G-X., ASADA K. 1994. Separate assays specific for ascorbate peroxidase and guaiacol peroxidase and for the chloroplastic and cytosolic isozymes of asciorbate peroxidase in plants. Plat Cell Physiology 35: 497-504.
• APEL K., HIRT H. 2004. Reactive oxygen species: Metabolism, oxidative stress, and signal transduction. Ann. Rev. Plant Biol. 55: 373-399.
• ARONSON L.J., GOLD A.J., HULL R.J. 1987. Cool-season turfgrass responses to drought stress. Crop Sci. 27: 1261-1266. ASADA K. 1992. Ascorbate peroxidase - a hydrogen peroxidescavenging enzyme in plants. Physiologia Plantarum 85: 235-241.
• ASHRAF M., FOOLAD M.R. 2007. Roles of glycine betaine and proline in improving plant biotic stress resistance. Environ. Exp. Bot. 59: 206-216.
• BANDURSKA H. 2000. Does proline accumulated in leaves of water deficit stressed barley plants confine cell membrane injury? I. Free proline accumulation and membrane injury index in drought and osmotically stressed plants. Acta Physiol. Plant. 22: 409-415.
• BATES L.S., Waldren R.P., Teare J.D. 1973. Rapid determination of proline for water stress studies. Plant and Soil 39: 205-207.
• BLUM A. 2005. Drought resistance, water-use efficiency, and yield potential - are they compatible, dissonant, or mutually exclusive. Aust. J. Agr. Res. 56: 1159-1168.
• BRADFORD M. 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254. BRAY E. 1997. Plant responses to water deficit. Trends Plant Sci. 2: 48-54.
• CHAVES M.M., MAROCO J.P., PEREIRA J. 2003. Understanding plant responses to drought - from genes to the whole plant. Functional Plant Biol. 30: 239-264.
• DHINDSA R., MATOWE W. 1981. Drought tolerance in two mosses: correlated with enzymatic defense against lipid peroxidation. J. Exp. Bot. 32: 79-91.
• FAROOQ M., WAHID A., KOBAYASHI N., BASRA S.M.A. 2009. Plant drought stress: effects, mechanisms and management. Agron. Sustain. Dev. 29: 185-212.
• FU J., HUANG B. 2001. Involvement of antioxidants and lipid peroxidation in the adaptation of two cool-season grasses to localizes drought stress. Environ. Exp. Bot. 45:105-114.
• GOLIŃSKA B., GOLIŃSKI P. 2005. Sodding potentials of different taxonomical forms of Festuca rubra. In: Frey L. (ed.): Biology of grasses. Pp. 379-384. W. Szafer Institute of Botany, Polish Academy of Science, Kraków.
• GOLIŃSKI P., DOMAŃSKI W. 2005. Variability of selected biological features and seed potentials of Festuca rubra. In: Frey L. (ed.): Biology of grasses. Pp. 385-391. W. Szafer Institute of Botany, Polish Academy of Science, Kraków.
• GUPTA S., GUPTA N.K., SHARMA M.I., PUROHIT A.K. 2005. Water stress induced antioxidant defense mechanism in seedlings of contrasting genotypes. J. Plant Biol. 32: 143-146.
• HAMMERSCHMIDT R., NUCLES E.M., KUC J. 1982. Association of enhanced peroxidase activity with induced systemic resistance of cucumber to Colletotrichum lagenarium. Physiol. Plant Pathol. 20: 73-82.
• HEATH R.L., PACKER L. 1968. Effect of light on lipid peroxidation in chloroplasts. Biochem. Biophys. Res. Commun. 19: 716-720.
• HIRAGA S., SASAKI K., ITO H., OHASHI Y., MATUSI H. 2001. A large family of class II plant peroxidases. Plat Cell Physiology 42: 462-468.
• HUANG B. GAO H. 1999. Physiological responses of diverse tall fescue cultivars to drought stress. HortSci. 34: 897-901.
• HUANG B., FRY J.D., WANG B. 1998. Water relations and canopy characteristics of tall fescue cultivars during and after drought stress. HortSci. 33: 837-840.
• JAIN M., MATHUR G., KOUL S., SARIN N.B. 2001. Ameliorative effects of proline on salt stress induced lipid peroxidation in cell lines of groundnut (Arachis hypoea L.). Plant Cell Rep. 20: 463-468.
• JIANG Y., HUANG B. 2000. Effects of drought or heat stress alone and in combination on Kentucky bluegrass. Crop Sci. 40: 1358-1362.
• JIANG Y., HUANG B. 2001. Drought and heat stress injury to two cool-season turfgrasses in relation to antioxidant metabolism and lipid peroxidation. Crop Sci. 41: 436-442.
• KARSTEN H.D., MacADAM J.W. 2001. Effect of drought on growth, carbohydrates, and soil water use by perennial ryegrass, tall fescue, and white clover. Crop Sci. 41: 156-166.
• KAVI KISHOR P.B., SANGAM S., AMRUTHA R.N., SRI LAXMI P., NAIDU K.R., RAO K.R.S.S., RAO S., REDDY K.J., THERIAPPAN P., SREENIVASULU N. 2005. Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: Its implications in plants growth and abiotic stress tolerance. Current Sci. 88: 424-438.
• KOSCY G., LAURIE R., SZALAI G., SZILGYI V., SIMON-SARKADI L., GALIBA G., RONDE J.A. 2005. Genetic manipulation of proline levels affects antioxidants in soybean subjected to simultaneous drought and heat stresses. Physiol. Plant. 124: 227-235.
• LIMA L.A.S., DAMATTA F.M., PINHERIO H.A., TOTOLA M.R., LOURERIO M.E. 2002. Photochemical responses and oxidative stress in two clones of Coffea canephora under water deficit conditions. Environ. Exp. Bot. 47: 239-247.
• LU S., WANG Z., NIU Y., GUO Z. 2008. Antioxidant responses of radiation-induced dwarf mutants of bermudagrass to drought stress. J. Amer. Soc. Hort. Sci. 133: 360-366.
• LUCERO D.W., GRIEU P., GUCKERT A. 1999. Effects of water deficit and plant interaction on morphological growth parameters and yield of white clover (Trifolium repens L.) and ryegrass (Loliumperenne L.) mixtures. Eur. J. Agron. 11: 167-177.
• MAHAJAN S., TUTEJA N. 2005. Cold, salinity and drought stresses: An overview. Archives of Biochemistry and Biophysics 444: 139-158.
• MARTINELLO P., D’ANDREA E. 2006. Cool-season turf grass species adaptability in Mediterranean environments and quality traits of varieties. Eur. J. Agron. 25: 234-242.
• MIKA A., LÜTHJE S. 2003. Properties of guaiacol peroxidase activities isolated from corn root plasma membranes. Plant Physiology 132: 1489-1498.
• MITTLER R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci. 7: 405-410.
• MOUSSA H.R., ABDEL-AZIS S.M. 2008. Comparative response of drought sensitive maize genotypes to water stress. Aust. J. Crop Sci. 1: 31-36.
• NAKANO Y., ASADA K. 1987. Purification of ascorbate peroxidase, its inactivation in ascorbate - depleted medium and reactivation by monodehydroascorbate radical. Plant Cell Physiol. 28: 131-14.
• NAYYAR H. 2004. Variation in osmoregulation in differently drought-sensitive wheat genotypes involves calcium. Biol. Plant. 47: 541-547.
• ÖZTÜRK L., DEMIR Y. 2002. In vivo and in vitro protective role of proline. Plant Growth Regul. 38: 259-264.
• SAIRAM R.K., SHUKLA D.S., SAXENA D.C. 1997. Stress induced injury and antioxidant enzymes in relation to drought tolerance in wheat genotypes. Biol. Plant. 40: 357-364.
• SAIRM R.K., SRIVASTAVA G.C. 1997. Stress induced injury and antioxidant enzymes in relation to drought tolerance in wheat genotypes. Biologia Plantarum 40: 357-364
• SERRAJ R., SINCLAIR T.R. 2002. Osmolyte accumulation: can it really help increase crop yield under drought conditions? Plant, Cell Environ. 25: 333-341.
• SHARMA P., DUBEY R.S. 2004. Ascorbate peroxidase from rice seedlings: properties of enzyme isoforms, effects of stresses and protective roles of osmolytes. Plant Sci. 167: 541-550.
• SHEFFER K.M., DUNN J.H., MINNER D.D. 1987. Summer drought response and rooting depth of tree cool-season tur- fgrasses. HortSci 22: 296-297.
• SMIRNOFF N. 1993. The role of active oxygen in the response of plants to water deficit and desiccation. New Phytol. 125: 27-58.
• SMIRNOFF N., CUMBES Q.J. 1989. Hydroxyl radical scavenging activity of compatible solutes. Phytochem. 28: 1057-1060, 1989.
• STADELMANN F.J., BOLLER B., SPANGENBERG G., KOLLIKER R., MESSERLII M., WANG Z.Y., NOSBERGER J. 1998. Fertility and growth in the field of Lolium perenne and Festuca rubra plants regenerated from suspension cultured cells and protoplasts. Plant Breed. 117: 37-43.
• TERZI R., KADIOLGLU A. 2006. Drought stress tolerance and the antioxidant enzyme system in Ctenanthe setosa. Acta Biol. Cracovensia, Series Bot. 48: 89-96.
• TROVATO M., MATIOLI R., COSTANTINO P. 2008. Multiple roles of proline in plant stress tolerance and development. Rendiconti Lincei 19: 325-346.
• TUBIELLO N., SOUSANA J.E., HOWDEN S.M. 2007. Crop and pasture response to climatic change. Proc. Nat. Acad. Sci. 104: 19686-19690.
• TÜRKAN I., BOR M., ÖZDEMIR F., KOCA H. 2005. Differential responses of lipid peroxidation and antioxidants in the leaves of drought-tolerant P. acutifolius Gray and drought-sensitive P. vulgaris L. subjected to polyethylene glycol mediated water stress. Plant Sci. 168: 223-231.
• VAN BREUSEGEM F., VRANOVA E., DAT J.F., INZE D. 2001.The role of active oxygen species in plant signal transduction. Plant Sci. 161: 405-414.
• VOLAIRE F. 2008. Plant traits and functional types to characterize drought survival of pluri-specific perennial herbaceous swards in Mediterranean areas. Eur. J. Agron. 29: 116-124.
• VOLAIRE F., THOMAS H., LELIEVRE F. 1998. Survival and recovery of perennial forage grasses under prolonged Mediteranean drought. I. Growth, death, water relations and solutes content in herbage and stubble. New Phytol. 140: 439-449.
• WANG Z-YL., GE Y. 2005. Recent advances in genetic transformation of forage and turf grasses. In Vitro Cellular and Developmental Biology - Plant 42: 1-18.
• WHITE R.H., ENGELKE M.C., MORTON S.J., RUEMMELE B.A. 1992. Competitive turgor maintenance in tall fescue. Crop Sci. 32: 251-256.
• YOSHIMURA K., YABUTA Y., ISHIKAWA T., SHIGROKA S. 2000. Expression of spinach ascorbate peroxidase isoenzymes in response to oxidative stresses. Plant Physiology 123: 223-233.