Effect of Solcoseryl on the apoptosis in the lungs of experimentally irradiated rats

• Autorzy: Staroslawska E , Donica H. , Kozaczynski W. , Wojcierowski J. , Jarmolinska K. , Wolanska E. , Bozyk A.
• Języki publikacji: EN

Abstrakty

EN

Modifying effect of Solcoseryl upon the late postirradiation damage of the lungs expressed as the number of apoptotic cells per 1mm² of the pulmonary tissue was investigated in rats. The number of labelled apoptotic cells in situ was determined by TUNEL method under light microscope connected to computer imaging (Lucia software). The results of the experiment demonstrated statistically significant reduction in the number of apoptotic cells in the pulmonary tissue in the group of rats irradiated with simultaneous administration of Solcoseryl in comparison to the group of animals irradiated without Solcoseryl treatment. The results indicate that Solcoseryl exhibits radioprotective activity upon the respiratory epithelium due to the reduction of the apoptotic cell number.

Słowa kluczowe

EN

ionizing radiation; TUNEL method; Solcoseryl; treatment; apoptotic cell; lung; apoptosis; rat; irradiated rat

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2001
• Tom: 45
• Numer: 1
• Opis fizyczny: p.67-73,fig.

Twórcy

autor

Staroslawska E

• University Medical School, 20-950 Lublin, Poland

autor

Donica H.

autor

Kozaczynski W.

autor

Wojcierowski J.

autor

Jarmolinska K.

autor

Wolanska E.

autor

Bozyk A.

Bibliografia

• 1. Burger A., Lóffler H., Bamberg M., Rodemann H.P.: Molecular and cellular basis of radiation fibrosis. Int. J. Radiat. Biol., 1998, 73, 401-408.
• 2. Coggle J.E., Lambert B.E., Moores S.R.: Radiation effects in the lung. Environ. Health Persp., 1986, 70, 261-291.
• 3. Cohen J. J.: Apoptosis. Immunol. Today. 1993, 14, 126-130.
• 4. Davis S.D., Yankelvitz F., Henschke C.I.: Radiation effects in the lung: Clinical features, pathology imaging findings. AJR, 1992, 159, 1157-1164.
• 5. Duguid J.B., Young A., Cauna D.: The internal surface area of the lung in emphysema. J. Path. Bac., 1964, 88, 405.
• 6. Dunnill M.S.: Quantitative methods in the study of pulmonary pathology. Thorax. 1962, 17, 320-324.
• 7. Elkind M., Sutton H.: X-ray and recovery in mammalian cells in culture. Nature, 1959, 184, 1293-1297.
• 8. Gavrieli Y., Sherman Y., Ben-sasson S.A.: Identification of programmed death cell in situ via specific labeling of nuclear DNA fragmentation. J. Cell Biol., 1992, 119, 493-501.
• 9. Gross N.J.: Pulmonary effects of radiation therapy. Ann. Intern. Med., 1997, 86, 81-91.
• 10. Hellman S.: Principles of cancer management: radiation therapy. In: Cancer Principles and Practice of Oncology. (V.T.de Vita, S.Hellman and S.A. Rosenber), 5th edition. Lippincott-Raven Publishers Philadelphia. New York , 1997, pp.307-325.
• 11. Mcbride W.H., Withers H.R., Davis C.A.: In vitro studies of anti-mortem proliferation kinetics. Br. J. Cancer., 1986, 53 (suppl.), 386.
• 12. Peitsch M.C., Mueller C., Tschopp J.: DNA fragmentation during apoptosis is caused by frequent single-strand cuts. Nucl. Acids Res., 1993, 21, 4206-4209.
• 13. Peitsch M.C., Mannherz H.G., Tschopp J.: The apoptosis endonucleases:cleaning up after cell death?. Trends Cell. Biol., 1994, 4, 37-41.
• 14. Rübe C.E., Rübe CH., Uthe D., Schmid K.W.: Expresion of inflammatory and profibrotic cytokines after whole lung irradiation. Exp. Strahlentherapie Klin. Strahlenbiologie, 1999, 8, 28-30.
• 15. Schwartz L. M.: The role of cell death genes during development. Bio Essays., 1991, 13, 389-395.
• 16. Sigdestad C.P., Weber K.: Differential protection of radiation-induced DNA single-strand breaks and cell survival by Solcoseryl. Experientia, 1988, 44, 707-708.
• 17. Smets L.A.: Programmed cell death (apoptosis) and response to anti-cancer drugs. Anti-Cancer Drugs, 1994, 5, 3-9.
• 18. Starosławska E., Chyczewski L., Pęszyński J., Sobolewski K.: Solcoseryl prevents early postradiation changes in the lungs. Pat. Pol., 1994, 45, 213-219.
• 19. Williams G.T., Smith C.: Molecular regulation of apoptosis. Genetic control of cel death. Cell, 1993, 74, 777-779.
• 20. Wyllie A.H.: Apoptosis and the regulation of cell numbers in normal and neoplastic tissues: an overview. Cancer Metast. Rev., 1992, 11, 95-103.