Autonomous endosperm development in unpollinated ovaries of Brassica napus L. cv. Topas cultured in vitro

• Autorzy: Rojek J. , Kuta E. , Przywara L.
• Języki publikacji: EN

Abstrakty

EN

Autonomous endosperm (AE) was found in 39 (17.3%) of in vitro cultured unpollinated ovaries of Brassica napus L. cv. Topas, with average frequency 1.5 ovule with AE per ovary. AE was induced on MS medium and on MS + 2 mg/l BAP + 0.1 mg/l NAA , MS + 2 mg/l BAP + 2 mg/l 2,4-D, and in ovules exposed to 1 h auxin treatment (MS + 20 or 40 mg/l 2,4-D) and then transferred to hormone-free MS medium. The highest frequency of AE induction occurred after 1 h auxin treatment (34.5%). The structure and size of AE nuclei suggest their origin from secondary nucleus or polar nucleus/nuclei. The control experiment with unpollinated, bagged flower buds left on plants, revealed no AE induction in situ. Gynogenic embryos were not observed in the material studied.

Słowa kluczowe

EN

unpollinated ovary; oilseed rape; ovary; Topas cultivar; Brassica napus; unpollinated ovule; development; autonomous endosperm; endosperm; in vitro

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2002
• Tom: 44
• Opis fizyczny: p.195-202,fig.,ref.

Twórcy

autor

Rojek J.

• University of Gdansk, Kladki 24, 80-822 Gdansk, Poland

autor

Kuta E.

autor

Przywara L.

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