Effect of Ca2 on the activity of mitochondrial NADP-specific isocitrate dehydrogenase from rabbit adrenals

• Autorzy: Strumilo E.
• Języki publikacji: EN

Abstrakty

EN

Mitochondrial NADP-specific isocitrate dehydrogenase (ICDH) partially purified from rabbit adrenals is sensitive to low concentrations of Ca2+. In the absence of Ca2+ (addition of 1 mM EGTA) the Km of ICDH for DL-isocitrate was 143 jaM, and in the presence of about 10 jaM Ca2+ (EGTA-Ca2+ buffer) it was decreased to 55 ¿iM. The JCm value of ICDH for NADP was similar in the absence (4.4 jxM) and in the presence (5.0 jiM) of Ca2+. The Km of mitochondrial NADP-ICDH for DL-isocitrate was higher than that of cytoplasmic NADP-ICDH from the same source.

Słowa kluczowe

EN

calcium; adrenal gland; rabbit; mitochondrial isocitrate dehydrogenase

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1995
• Tom: 42
• Numer: 3
• Opis fizyczny: p.325-328,fig

Twórcy

autor

Strumilo E.

• Academy of Sciences of Belarus, BLK-50, 230017 Grodno, Belarus

Bibliografia

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