Wybrane cechy chorobotworczosci oraz zroznicowanie genomowe szczepow Staphylococcus aureus wyizolowanych z mleka krow z zapaleniem gruczolu mlekowego w regionie polnocno-wschodniej Polski

• Autorzy: Dziekiewicz-Mrugasiewicz M , Rzewuska M. , Stefanska I. , Jakubczak A.

Warianty tytułu

EN

Select pathogenic characteristics as well as the genotypic diversity of Staphylococcus aureus strains isolated from milk of cows with mastitis in the north-east region of Poland

• Języki publikacji: PL

Abstrakty

EN

The aim of this study was the characterization of Staphylococcus aureus strains isolated from milk of cows with mastitis in the north-east region of Poland. Genotyping of 45 isolates by ADSRRS-fingerprinting showed nine different patterns of bands. Genotype D was predominant and 29 S. aureus strains (65.5%) were classified to this genotype. These strains of genotype D had strong pathogenicity, because in the first year of the study only 14.29% isolates belonged to D and in the next year 73.64% isolates. The strains of the predominant genotype showed stronger pathogenic properties then the strains of other genotypes. The majority of them showed a significant adhesion to the epithelial cells of the mammary gland, and the strong ability to produce slime and to form biofilm.

Słowa kluczowe

PL

krowy; zapalenie gruczolu mlekowego; mleko; zanieczyszczenia bakteryjne; Staphylococcus aureus; szczepy bakteryjne; roznicowanie; genotyp; chorobotworczosc; adhezja; biofilmy bakteryjne; sluz; metoda ADSRRS-fingerprinting; Polska Polnocno-Wschodnia

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2008
• Tom: 64
• Numer: 08
• Opis fizyczny: s.1012-1015,rys.,tab.,bibliogr.

Twórcy

autor

Dziekiewicz-Mrugasiewicz M

• Szkola Glowna Gospodarstwa Wiejskiego, ul.Nowoursynowska 159 C, 02-787 Warszawa

autor

Rzewuska M.

autor

Stefanska I.

autor

Jakubczak A.

Bibliografia

• 1. Aguilar B., Amorena B., Iturralde M.: Effect of slime on adherence of Staphylococcus aureus isolated from bovine and ovine mastitis. Vet. Microbiol. 2001, 78, 183-190.
• 2. Amorena B., Garcia E., Monzün M., Leiva J., Oteiza C., Pérez M., Alabart J. L., Hernández-Yago J.: Antibiotic susceptibility assay for Staphylococcus aureus in biofilms developed in vitro. J. Antimicrobial Chem. 1999, 44, 43-55.
• 3. Barańska-Rybak W.: Amplification of DNA fragments surrounding rare restriction sites (ADSRRS-fingerprinting) for typing Staphylococcus aureus isolated from patients with recurrent furunculosis. ECCMID Meeting, Prague, Czech Republic, Poster 2004.
• 4. Baselga R., Albizu I., De La Cruz M., Cacho E., Barberan M., Amorena B.: Phase variation of slime production in Staphylococcus aureus: implications in colonization and virulence. Infect. Immun. 1993, 61, 4857-4862.
• 5. Buzzola F. R., Quelle L., Gomez M. I., Catalano M., Steele-Moore L., Berg D., Gentilini E., Denamiel G., Sordelli D. O.: Genotyping analysis of Staphylococcus aureus from milk of dairy cows with mastitis in Argentina. Epidemiol. Infect. 2001, 126, 445-452.
• 6. Caputy C., Costerton J. W.: Immunological examination of glycocalyces of Staphylococcus aureus strains Willey and Smith. Current Microbiol. 1984, 11, 297-302.
• 7. Cifrian E., Guidry A. J., Bramley A. J., Norcross N. L., Bastida-Corcuera F. D., Marquardt W. W.: Effect of staphylococcal β toxin on the cytotoxicity, proliferation and adherence of Staphylococcus aureus to bovine mammary epithelial cells. Vet. Microbiol. 1996, 48, 187-198.
• 8. Dziekiewicz-Mrugasiewicz M.: Występowanie i charakterystyka szczepów Staphylococcus aureus izolowanych z mleka krów w regionie północno-wschodniej Polski. Praca dokt. SGGW, Warszawa 2007.
• 9. Fox L. K., Zadoks R. N., Gaskins C. T.: Biofilm production by Staphylococcus aureus associated with intramammary infection. Vet. Microbiol. 2005, 107, 295-299.
• 10. Freeman D. J., Falkiner F. R., Keane C. T.: New method for detecting slime production by coagulase negative staphylococci. J Clin. Pathol. 1989, 42, 872-874.
• 11. Frost A. J.: Selective adhesion of microorganisms to the ductular epithelium of bovine mammary gland. Infect. Immun. 1975, 12, 1154-1156.
• 12. Hébert A., Sayasith K., Sénéchal S., Dubreuil P., Lagacé J.: Demonstration of intracellular Staphylococcus aureus in bovine mastitis alveolar cells and macrophages isolated from naturally infected cow milk. FEMS Microbiol. Letters 2000, 193, 57-62.
• 13. Krawczyk B., Lewandowski K., Bronk M., Samet A., Myjak P., Kur J.: Evaluation of novel method based on amplification of DNA fragments surrounding rare restriction sites (ADSRRS-fingerprinting) for typing strains of vancomycin - resistant Enterococcus faecium. J. Microbiol. Methods. 2003, 52, 341-351.
• 14. Krawczyk B., Naumiuk Ł., Lewandowski K., Baraniak A., Gniadowski M., Samet A., Kur J.: Evaluation and comparison of random amplification of polymorphic DNA, pulsed-field gel electrophoresis and ADSRRS-fingerprinting for typing Serratia marcescens outbreaks. FEMS 2003, 38, 241-248.
• 15. Lam T. J., Lipman L. J., Schukken Y. H., Gaastra W., Brand A.: Epidemiological characteristics of bovine clinical mastitis caused by Staphylococcus aureus and Eschericha coli studied by DNA fingerprinting. Am. J. Vet. Res. 1996, 57, 39-42.
• 16. Lange C., Cardoso M., Senczek D., Schwarz S.: Molecular subtyping of Staphylococcus aureus from cases bovine mastitis in Brazil. Vet. Microbiol. 1999, 67, 127-141.
• 17. Lee J. C., Betley M. J., Hopkins C. A., Pérez N. E., Pier G. B.: Virulence studies, in mice, of transposoninduced mutans of Staphylococcus aureus differing in capsular size. J. Infect. Dis. 1987, 156, 935-939.
• 18. Lipman L., Nijs de A., Lam T., Rost J., Dijk von L., Schukken Y., Gaastra W.: Genotyping by PCR, of Staphylococcus aureus strains, isolated from mammary gland of cows. Vet. Microbiol. 1996, 48, 51-55.
• 19. Malinowski E.: Przyczyny, leczenie i zapobieganie mastitis u krów. PIWet Puławy 1997.
• 20. Malinowski E., Kłosowska A.: Diagnostyka zakażeń i zapaleń wymienia. PIWet Puławy 2002.
• 21. Malinowski E., Lassa H., Kłossowska A., Smulski S., Markiewicz H., Kaczmarowski M.: Etiological agents of dairy cows mastitis in western part of Poland. Pol. J. Vet. Sci. 2006, 9, 191-194.
• 22. Masny A., Płucienniczak A.: Fingerprinting of bacterial genomes by amplification of DNA fragments surrounding rare restriction sites. BioTechniques. 2001, 13, 930-936.
• 23. Matthews K. R., Jayarao B. M., Oliver S. P.: Plasmid pattern analysis of Staphylococcus aureus isolated from bovine mammary secretions. J. Dairy Sci. 1993, 75, 3318-3323.
• 24. Myllys V., Ridell J., Björkroth J., Biese I., Pyörälä S.: Persistence in bovine mastitis of Staphylococcus aureus clones as assessed by random amplified polymorphic DNA analysis, ribotyping and biotyping. Vet. Microbiol. 1997, 57, 245-251.
• 25. Raimundo O., Deighton M., Capstick J., Gerraty N.: Molecular typing of Staphylococcus aureus of bovine origin by polymorphisms of the coagulase gene. Vet. Microbiol. 1999, 66, 275-284.
• 26. Sachanowicz J., Jakubczak A., Kleczkowski M.: Różnicowanie szczepów Staphylococcus aureus metod¹ PCR-fingerprinting. Medycyna Wet. 2003, 59, 710-712.
• 27. Stefańska I., Rzewuska M., Nowicki M., Kaba J., Binek M.: Właściwości fizjologiczne i zmienność genomowa szczepów Corynebecterium pseudotuberculosis wyizolowanych od kóz. Medycyna Wet. 2007, 63 Suplement, 1474-1477.
• 28. Vasudevan P., Nair M., Annamalai T., Venkitanarayanan K. S.: Phenotypic and genotypic characterization of bovine mastitis isolates of Staphylococcus aureus for biofilm formation. Vet. Microbiol. 2003, 92, 179-185.
• 29. Zadoks R.: Comparison of Staphylococcus aureus isolated from bovine mastitis and human skin, milking equipment and bovine milk by phage typing, pulsed - field gel elektrophoresis and binary typing. J. Clin. Microbiol. 2002, 40 (11), 3894-3902.