Pdr12p-dependent and -independent fluorescein extrusion from baker's yeast cells

• Autorzy: Lushchak V. , Abrat O. , Miedzobrodzki J. , Semchyshyn H.
• Języki publikacji: EN

Abstrakty

EN

Fluorescein efflux from S. Cerevisiae cells was measured to study the peculiarities of fluorescein transport system, which is important for yeast resistance to certain drugs and weak organic acid preservatives. Glucose-independent and glucose-stimulated fluorescein effluxes were characterized using iodoacetate, cyanide and orthovanadate, inhibitors of glycolysis, electron transport chain, and ATPases, respectively. It is supposed that in glucose-free medium fluorescein extrusion is ATP-dependent and the energy for this efflux is mainly provided by respiration. In glucose-containing medium, glycolysis plays a critical role for extrusion of fluorescein. The results indicate that acetic acid inhibits the fluorescein efflux from yeast cells. The inhibition constant of glucose-stimulated fluorescein efflux is significantly lower in parental strain than in two mutants defective in PDR12 (ABC-transporter Pdr12p) or WAR1 (transcription factor of Pdr12p). It can be suggested that the membrane protein Pdr12 is involved in fluorescein extrusion from the yeast cells, but component(s) other than Pdr12p is (are) also important.

Słowa kluczowe

PL

Saccharomyces cerevisiae; yeast; fluorescein; extrusion; glucose; inhibitor; acetic acid; fluorescein diacetate; yeast cell; mutant; membrane protein

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2008
• Tom: 55
• Numer: 3
• Opis fizyczny: p.595-601,fig.,ref.

Twórcy

autor

Lushchak V.

• Vassyl Stefanyk Precarpathian National University, 57 Shevchenko Str., 76025, Ivano-Frankivsk, Ukraine

autor

Abrat O.

autor

Miedzobrodzki J.

autor

Semchyshyn H.

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