Detection of methicillin resistance in hospital environmental strains of coagulase-negative staphylococci

• Autorzy: Nowak T , Balcerczak E. , Mirowski M. , Szewczyk E.M.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to evaluate methicillin resistance detection methods currently used when studying coagulase-negative staphylococci (CoNS). The resistance to oxacillin of 142 strains from seven species of CoNS isolated from the Intensive Care Unit environments was tested. The methods used were: disc diffusion test with cefoxitin (FOX₃₀) and oxacillin (OX₁), oxacillin agar screen test with 6 mg/l of oxacillin (MHOXA), latex test for PBP2a (LA) and detection of mecA via PCR. One hundred and one isolates were methicillin-resistant in at least one of methods used, but only 74 were mecA -positive. Of the 68 mecX-negative strains: two were positive by OX₁, the LA and MHOXA methods; three by the LA and MHOXA; and 22 only by OX₁, test. Most of these strains were from the novobiocin-resistant CoNS group. The results obtained for all tested strains using FOX₃₀ showed complete concordance with the presence of the mecA gene.

Słowa kluczowe

EN

nosocomial infection; cefoxitin disc test; hospital strain; Staphylococcus epidermidis; hospital environment; methicillin resistance; coagulase-negative staphylococci; polymerase chain reaction; detection

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2006
• Tom: 55
• Numer: 4
• Opis fizyczny: p.339-343,ref.

Twórcy

autor

Nowak T

• Medical University, Pomorska 137, 90-235 Lodz, Poland

autor

Balcerczak E.

autor

Mirowski M.

autor

Szewczyk E.M.

Bibliografia

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