Wystepowanie i charakterystyka powierzchniowego bialka P-like paleczek Klebsiella

• Autorzy: Przondo-Mordarska A. , Smutnicka D. , Matusiewicz K.
• Języki publikacji: PL

Abstrakty

PL

Właściwości nowo opisanej adhezyny P-like Klebsiella badano metodami hemaglutynacyjnymi, mikroskopowo-elektronowymi i biochemicznymi. Stwierdzono amorficzny charakter adhezyny i jej swoistość receptorową zbliżoną do adhezyny PapG E.coli.

EN

Colonisation and remaining of microorganism on mucus membrane of microorganism is tightly connected with adhesion mechanisms and determine the first step of physiological settlement of the organism or the first stage of clinically demonstrated infection. In Klebsiella rods there are known three types of fimbrial adhesins (type 1, 3 and KPF-28) and non-fimbrial adhesin CF29K. It is stated that Klebsiella strains adhesions are responsible for their adherence to the epithelial cells of both respiratory and urinary tracts and to intestine epithelium. The in vitro research affirmed Klebsiella rods adherence to protein matrix. The aim of our work was the establishment of character, receptor specifity and the appearance frequency of P-like called adhesin. The frequency of expression of P-like adhesin was estimated among 380 isolated from the patients strains on the basis of agglutinating methods. The amorphic character of P-like adhesin was proved using electron microscopy method. The isolation and purification of P-like protein with a help of affinity chromatography enabled to estimate the receptor specifity of the adhesin. The receptor specifity was established as similar to E.coli PapG adhesin.

Słowa kluczowe

PL

metody hemaglutynacyjne; adhezyna PapG; wlasciwosci adhezyjne; Klebsiella oxytoca; metody biochemiczne; adhezyna P-like; adhezyny; Klebsiella pneumoniae

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2003
• Tom: 55
• Numer: 2
• Opis fizyczny: s.135-146,tab.,fot.,rys.,bibliogr.

Twórcy

autor

Przondo-Mordarska A.

• Akademia Medyczna, ul.T.Chalubinskiego 4, 50-368 Wroclaw

autor

Smutnicka D.

autor

Matusiewicz K.

Bibliografia

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