Distribution of NADPH-diaphorase and nitric oxide synthase in porcine ovaries after intraovarian infusions of Escherichia coli endotoxin

• Autorzy: Jana B. , Andronowska A. , Kucharski J.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to determine whether pathological changes in Escherichia coli endotoxin (LPS, lipopolysaccharide)-infused ovary are associated with an increase in NO production, and whether inflammatory mediators produced by LPS-infused ovary can affect the contralateral one to stimulate NO synthesis. Therefore, the activity of NADPH-diaphorase (NADPH-d) and distribution of the inducible isoform of nitric oxide synthase (iNOS) used as indicators of the intensity of the inflammatory process were studied in porcine ovaries after unilateral infusions of LPS into the hilus of one ovary. Fourteen sexually matured gilts with controlled estrous cycle were used. The animals were randomly divided into two groups: I (control; n=7), and II (treated; n=7). In the group I, 1ml of saline was infused into the hilus of each ovary, on the 15th to the 19th day of the estrous cycle, twice a day (at 06:00 and 18:00). The gilts of the II group received 2 mg of LPS (serotyp 055:B5) in 1 ml of saline infused into the hilus of one ovary on the same days of the estrous cycle. At the same time, 1 ml of saline was infused into the contralateral ovary. The ovaries were collected on the 7th day of the next estrous cycle during a median laparotomy. Cryostat sections of the paraformaldehyde fixed ovarian tissues were stained histochemically to study the activity of NADPH-d and immunohistochemi- cally to investigate the distribution of iNOS. In the control group, the activity of NADPH-d was similar either in the corpora lutea, thecal and granulosa cells of follicles or endothelial cells of blood vessels in both ovaries. In the LPS-infused ovary, the activity of this enzyme was higher (P < 0.05 - 0.01) in thecal and granulosa cells of follicles and in endothelium of the blood vessels as compared to that found in the corresponding structures of the contralateral ovary. The highest activity of NADPH-d was observed in luteinizing thecal and granulosa cells of cysts which occured in both ovaries only after LPS infusions. In all the structures of both ovaries, the intensity of NADPH-d reaction was higher (P < 0.05 - 0.001) in LPS-treated gilts than that found in the control group. Light microscopic observations revealed the strongest immunostaining for iNOS in the structures of LPS-infused ovary. In the contralateral ovary, iNOS im- munoreactivity was weaker but still stronger than that found in the control group. This study revealed that in gilts infusions of LPS into the hilus of one ovary enhanced the activity of NADPH-d and iNOS in both the ovaries. However, the activity of these enzymes depended on the location of LPS infusions. The data obtained indicate also that locally synthesised NO can mediate an inflammatory effect of LPS in the ovaries.

Słowa kluczowe

EN

pig; ovary; nitric oxide synthase; Escherichia coli; intraovarian infusion; endotoxin

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2000
• Tom: 03
• Numer: 3
• Opis fizyczny: p.153-159,fig.

Twórcy

autor

Jana B.

• Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland

autor

Andronowska A.

autor

Kucharski J.