Effect of hypoxia on photosynthetic activity and antioxidative response in gametophores of Mnium undulatum

• Autorzy: Rzepka A , Krupa J. , Slesak I.
• Języki publikacji: EN

Abstrakty

EN

The effects of hypoxia caused by complete submerging of Mnium undulatum gametophores in water, on their photosynthetic activity and the activity of two antioxidative en zymes: superoxide dismutase (SOD) and catalase (CAT) were investigated. The net photosynthesis was strongly inhibited throughout the experiment, and the strong drop in the maximum quantum yield of the PSII (Fv / Fm) was also observed. Three classes of SOD: MnSOD, FeSOD, Cu/ZnSOD and three isoforms of Cu/ZnSOD were identified. A significant decrease in activity of MnSOD, FeSOD and one Cu/ZnSOD isoform was observed after 24 and 48 h of hypoxia. FeSOD activity decreased already after 1 h of submerging in water and its activity remained at the low level during whole period of the experiment. CAT activity was also strongly inhibited in response to hypoxia stress. The obtained results suggest relationships between photosynthetic activity and antioxidative system in M. undulatum gametophores under oxygen deficiency stress.

Słowa kluczowe

EN

superoxide dismutase; plant physiology; chlorophyll a; fluorescence; antioxidative response; oxidative stress; hypoxia; catalase; Mnium undulatum; gametophore; photosynthesis; photosynthetic activity

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2005
• Tom: 27
• Numer: 2
• Opis fizyczny: p.205-212,fig.,ref.

Twórcy

autor

Rzepka A

• Pedagogical Academy, Podbrzezie 3, 30-054 Krakow, Poland

autor

Krupa J.

autor

Slesak I.

Bibliografia

• Aebi H. 1984. Catalase in vitro. In: Methods in Enzymology, Vol. 105, Academic Press, Inc: 121-126.
• Alscher R.G., Donahue J.L., Cramer C.L. 1997. Reactive oxygen species and antioxidants : relationships in green cells. Physiol. Plant. 100: 224-233.
• Alscher R.G., Erturk N., Heath L.S. 2002. Role of superoxide dismutases (SODs) in controlling oxidative stress in plants. J. Exp. Bot. 53: 1331-1341.
• Bartosz G. 1997. Oxidative stress in plants. Acta Physiol. Plant. 19: 47-64.
• Beauchamp C., Fridovich I. 1971. Superoxide dismu- tase: Improved assays and an assay applicable to acrylamide gels. Anal. Biochem. 44: 276-287.
• Blokhina O.B., Chirkova T.V., Fagerstedt K.V. 2001.
• Anoxic stress leads to hydrogen peroxide formation in plant cells. J. Exp. Bot. 52: 1179-1190.
• Blokhina O., Virolainen E., Fagerstedt K.V. 2003.
• Antioxidants, oxidative damage and oxygen deprivation stress: a review. Ann. Bot. 91: 179-194.
• Bowler C., van Montagu M., Inze D. 1992. Superoxide dismutase and stress tolerance. Annu. Rev. Plant. Physiol. Mol. Biol. 43: 83-116.
• Bradford M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein uti- lizmg the principle of protein-dye bindmg. Anal. Biochem. 72: 248-254.
• Bragina T.V., Drozdova I.S., Ponomareva Y.V., Alekhin V.I., Grineva G.M. 2002. Photosynthesis, respiration, and transpiration in maize seedlings under hypoxia induced by complete flooding. Dokl. Biol. Sci. 384: 274-277.
• Christov K., Bakardjieva N.T. 1999. Effect of calcium and zinc on subcelluar distribution, activity and thermosensitivity of superoxide dismutase in Mnium affine. Biol. Plant. 42: 57-63.
• Dat J., Vandenabeele S., Vranova E., Van Montagu M., Inze D., Van Breusegem F. 2000. Dual action of the active oxygen species during plant stress retponses. CMLS Cell Mol. Life Sci. 57: 779-795.
• Dat J.F., Capelli N., Folzer H., Bourgeande P., Badot P-M. 2004. Sensing and signalling during plant flooding. Plant Physiol. Biochem. 42: 273-282.
• Drew M.C. 1997. Oxygen deficiency and root metabolism: injury and acclimation under hypoxia and anoxia. Annu. Rev. Plant Physiol. Plant Mol. Biol. 48: 223-250.
• Foyer C.H., Noctor G. 2000. Oxygen processing in photosynthesis: regulation and signalling. New Phytol. 146: 359-388.
• Ladygin V.G. 1999. Functional activity and chloroplast structure in leaves of Pisum sativum and Glycine max under conditions of root hypoxia and anoxia. Rus. J. Plant Physiol. 46: 207-218.
• Liao C-T., Lin C-H. 2001. Physiological adaptation of crop plants to floodtng stress. Proc. Natl. Sci. Counc. ROC (B) 25: 148-157.
• Maxwell K., Johnson G.N. 2000. Chlorophyll fluorescence - a practical guide. J. Exp. Bot. 51: 659-668.
• Miszalski Z., Ślesak I., Niewiadomska E., Bączek- -Kwinta R., Luttge U., Ratajczak R. 1998. Subcellular localization and stress responses of superoxide dismu- tase isoforms from leaves in the C3-CAM intermediate halophyte Mesembryanthemum crystallinum L. Plant Cell Environ. 21: 169-179.
• Mittler R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci. 7: 405-410.
• Mustroph A., Albrecht G. 2003. Tolerance of crop plants to oxygen deficiency stress : fermentative activity and photosynthetic capactty of entire seedtings under hypoxia and anoxia. Physiol. Plant. 117: 508-521.
• Proctor M. 2001. Patterns of desiccation tolerance and recovery in bryophytes. Plant Growth Regul. 35: 147-156.
• Rzepka A ., Krupa J., Rut G. 2002. The influence of anareobic conditions on the dark respiration of moss gametophytes. Zesz. Probl. Post. Nauk Rol. 481 : 251-258.
• Scandalios J.G. 1994. Regulation and properties of plant catalases. In: Causes of photooxidative stress and amehoration of defense systems in plants. Foyer Ch., Mullineaux P. M., (ed.), Boca Raton, Florida: CRC Press: 275-316.
• Schluter U., Crawford R.M.M. 2001. Long-term anoxia tolerance in leaves of Acorus calamus L. and Iris pseudacorus L. J. Exp. Bot. 52: 2213-2225.
• Ślesak I., Miszalski Z. 2003. Superoxide dismutaset -like protein from roots of the intermediate C3-CAM plant Mesembryanthemum crystallinum L. in in vitro culture. Plant Sci. 164: 497-505.
• Ushimaru T., Ogawa K., Ishida N., Shibasaka M., Kanematsu S., Asada K., Tsuji H. 1995. Changes in organelle superoxide dismutase isoenzymes duri ng air adaptation of submerged rice seedlings: differential behaviour of isoenzymes in plastids and mitochondria. Planta 196: 606-613.
• Ushimaru T., Kanematsu S., Shibasaka M., Tsuji H. 1999. Effect of hypoxia on the antioxidative enzymes in aerobically grown rice (Oryza sativa) seedlings. Physiol. Plant. 107: 181-187.
• Ushimaru T., Kanematsu S., Katayama M., Tsuji H. 2001. Antioxidative enzymes in seedtings of Nelumbo nucifera germinated under water. Physiol. Plant. 112: 39-46.
• Willekens H., Inze D., Van Montagu M., Van Camp W. 1995. Catalases in plants. Mol. Breed. 1: 207-228.
• Yamahara T., Shiono T., Suzuki T., Tanaka K., Takio S., Sato K., Yamazaki S., Satoh T. 1999. Isolation of a germin-like protein with manganese superoxide dismutase activity from cells of a moss, Barbula unguiculata. J. Biol. Chem. 274: 33274-33278.
• Yordanova R.Y., Alexieva V.S., Popova L.P. 2003. Influence of root oxygen deficiency on photosynthesis and antioxtdant status in bartey plants. Rus. J. Plant Physiol. 50: 163-167.
• Vartapetian B.B., Jackson M.B. 1997. Plant adaptations to anaerobic stress. Ann. Bot. 79: 3-20.