Expression of small synthetic genes coding for hEGF, human epidermal growth factor, and CPTI II, serine proteinase inhibitor from Cucurbitacea, cloned in a novel expression-secretion vector in Saccharomyces cerevisiae

• Autorzy: Topczewska J. , Rempola B. , Fikus M.
• Języki publikacji: EN

Abstrakty

EN

Efficient synthesis of two small eukaryotic polypeptides of human and plant origin was carried out using a novel expression/secretion yeast vector, pYET. The yield was optimized in respect of the yeast strain, expression cassette construction, promoter regulation and culture conditions. Both cloned genes code for biotechnologically important proteins: human epidermal growth factor and a serine proteinase inhibitor from Cucurbitacea.

Słowa kluczowe

EN

epidermal growth factor; yeast; serine proteinase inhibitor; synthetic gene; clone; man; culture condition; Cucurbitacea; Saccharomyces cerevisiae; plant

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1996
• Tom: 43
• Numer: 1
• Opis fizyczny: p.255-264,fig.

Twórcy

autor

Topczewska J.

• Polish Academy of Sciences, A.Pawinskiego 5A, 02-106 Warsaw, Poland

autor

Rempola B.

autor

Fikus M.

Bibliografia

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