Autolysis of Listeria monocytogenes strains isolated from food and clinical specimens

• Autorzy: Cibik R , Cetinkaya F. , Gunes N. , Ozakin C. , Soyutemiz G.E.
• Języki publikacji: EN

Abstrakty

EN

Autolysis of Listeria monocytogenes strains isolated from clinical and food specimens were tested under starvation conditions. Late exponential phase harvested bacterial cells were transferred in potassium phosphate buffer (100 mM, pH 7) and incubated at 37°C to induce lysis. At the end of the 48 h incubation period a heterogeneous autolysis profile was observed among the tested strains. All the clinical strains exhibited high autolysis i.e. above 75%. Meanwhile, the extent of food isolates was not as high as the clinical ones but demonstrated a great variability - ranging between 22 to 88%. An increase in the amount of intracellular markers upon lysis was also measured in the higher autolytic clinical strain - UVF172, compared to low autolytic food strain - UVF114. The results obtained from the study provided evidence that autolysis in L. monocytogenes is strain dependent and revealed that tested clinical isolates had a higher level of autolysis than food isolates.

Słowa kluczowe

EN

food; Listeria monocytogenes; bacterial pathogen; autolysis; isolation; clinical specimen; food specimen; potassium phosphate; determination

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2006
• Tom: 62
• Numer: 11
• Opis fizyczny: p.1242-1244,ref.

Twórcy

autor

Cibik R

• University of Uludag, Gorukle, 16059 Bursa-Turkey

autor

Cetinkaya F.

autor

Gunes N.

autor

Ozakin C.

autor

Soyutemiz G.E.

Bibliografia

• 1.Anon.: Minitab Statistical Software. Minitab Release 13.20, Minitab Inc. 2000.
• 2.Ayres H. M., Furr J. R., Russell A. D.: Effect of divalent cations on permeabilizer-induced lysozyme lysis of Pseudomonas aeruginosa. Lett. Appl. Microbiol. 1998, 27, 372-374.
• 3.Canvin J. R., Marvin A. P., Sivakumaran M., Paton J. C., Boulnois G. J., Andrew P. W., Mitchell T. J.: The role of pneumolysin and autolysin in the pathology of pneumonia and septicaemia in mice infected with a type 2 pneumococcus. J. Infect. Dis. 1995, 172, 119-123.
• 4.Chapot-Chartier M. P.: Les autolysines des bactéries lactiques. Lait 1996, 76, 91-109.
• 5.Cheribun C. E., Appelman M. D., Heseltine P. N. R., Khayr W., Stratton C. W.: Epidemiology spectrum and current treatment of listeriosis. Rev. Infect. Dis. 1991, 13, 1108-1114.
• 6.Cibik R., Chapot-Chartier M. P.: Autolysis of dairy leuconostocs and detection of peptidoglycan hydrolases by renaturing SDS-PAGE. J. Appl. Microbiol. 2000, 89, 862-869.
• 7.Farber J. M., Peterkin P.: Listeria monocytogenes, a foodborne pathogen. Microbiol. Rev. 1991, 55, 476-511.
• 8.Gaillard J. L., Jaubert F., Berche P.: The inlAB locus mediates the entry of Listeria monocytogenes into hepatocytes in vivo. J. Exp. Med. 1996, 183, 359-369.
• 9.Hebeler B. H., Young F. E.: Autolysis of Neisseria gonorrhoeae. J. Bacteriol. 1975, 122, 385-392.
• 10.Kang O. J., Vezinz L. P., Laberge S., Simard R. E.: Some factors influencing the autolysis of Lactobacillus bulgaricus and Lactobacillus casei. J. Dairy Sci. 1998, 81, 639-646.
• 11.Kathariou S.: Listeria monocytogenes virulence and pathogenicity, a food safety perspective. J. Food. Protect. 2002, 65, 1811-1829.
• 12.Lemee R., Rouault A., Guezenec S., Lortal S.: Autolysis of 57 strains of dairy propionibacteria. Lait 1994, 74, 41-51.
• 13.Mani N., Baddour L. M., Offutt D. Q., Vijaranakul U., Adakavukaren M. J., Jayaswal R. K.: Autolysis-detective mutant of Staphylococcus aureus: pathological considerations, genetic mapping, and electron microscopic studies. Infect. Immun. 1994, 62, 1406-1409.
• 14.Martinez I., Rorvik L. M., Brox V., Lassen J., Sappola M., Gram L., Fonnesbech-Vogel B.: Genetic variability among isolates of Listeria monocytogenes from food products, clinical samples and processing environments, estimated by RAPD typing. Int. J. Food. Microbiol. 2003, 84, 285-297.
• 15.Meng J., Doyle M. P.: Emerging and evolving microbial foodborne pathogens. Bull. Inst. Pasteur. 1998, 96, 151-164.
• 16.Roche S. M., Gracieux P., Albert I., Gouali M., Jacquet C., Martin P. M. V., Velge P.: Experimental validation of low virulence in field strains of Listeria monocytogenes. Infect. Immun. 2003, 71, 3429-3436.
• 17.Roche S. M., Velge P., Bottreau E., Durier C., Marquet Van Der Mee N., Pardon P.: Assesment of the virulence of Listeria monocytogenes: agreement between a plaque-forming assay with HT-29 cells and infection of immunocompetent mice. Int. J. Food Microbiol. 2001, 68, 33-44.
• 18.Shockman G. D., Höltje J. V.: Microbial peptidoglycan (murein) hydrolases, [in:] Ghuysen J. M., Hakenbeck R. (Eds.): Bacterial Cell Wall. Elsevier Science, Amsterdam 1994, pp. 131-166.
• 19.Tobin P. J., Mani N., Jayaswal R. K.: Effect of physiological conditions on the autolysis of Staphylococcus aureus strains. Anton. Leeuw. Int. J. G. 1994, 65, 71-78.
• 20.Valisena S., Varaldo P. E., Satta J.: Staphylococcal endo-b-N acetylglucosaminidase inhibits response of human lymphocytes to mitogens and interferes with production of antibodies in mice. J. Clin. Invest. 1991, 87, 1969-1976.
• 21.Watt S. R., Clarke A. J.: Role of autolysins in the EDTA-induced lysis of Pseudomonas aeruginosa. FEMS Microbiol. Lett. 1994, 124, 113-120.
• 22.Wuenscher M. D., Köhler S., Bubert A., Gerike U., Goebel W.: The iap gene of Listeria monocytogenes is essential for cell viability, and its gene product, p60, has bacteriolytic activity. J. Bacteriol. 1993, 175, 3491-3501.