Changes in GSH-antioxidant system induced by daunorubicin in human normal and diabetic fibroblasts

• Autorzy: Zatorska A. , Maszewski J. , Jozwiak Z.
• Języki publikacji: EN

Abstrakty

EN

We investigated the effect of daunorubicin on glutathione content and activity of GSH-related enzymes in cultured normal and diabetic human fibroblasts. Cells were incubated with 4 ^M daunorubicin (DNR) for 2 h followed by culture in drug-free me­dium for up to 72 h. Treatment of diabetic cells with the drug caused a time-dependent depletion of intracellular GSH and a decrease of the GSH to total glutathione ratio. GSH depletion was accompanied by apoptotic changes in morphology of the nucleus. Analysis of GSH-related enzymes showed a significant increase of the activities of Se-dependent and Se-independent peroxidases and glutathione ^-transferase. In con­trast, glutathione reductase activity was reduced by 50%. Significant differences be­tween normal and diabetic cells exposed to DNR were observed in the level of GST and Se-dependent glutathione peroxidase activities. These findings indicated that daunorubicin efficiently affects the GSH antioxidant defense system both in normal and diabetic fibroblasts leading to disturbances in glutathione content as well as in the activity of GSH-related enzymes.

Słowa kluczowe

EN

glutathione S-transferase; man; daunorubicin; antibiotic; oxidative stress; glutathione reductase; glutathione; normal fibroblast; apoptosis; fibroblast; glutathione peroxidase; diabetic fibroblast

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2003
• Tom: 50
• Numer: 3
• Opis fizyczny: p.825-835,fig.

Twórcy

autor

Zatorska A.

• University of Lodz, S.Banacha 12-16, 90-237 Lodz, Poland

autor

Maszewski J.

autor

Jozwiak Z.

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