In vivo gene transfer using cetylated polyethylenimine

• Autorzy: Sochanik A. , Cichon T. , Makselon M. , Strozyk M. , Smolarczyk R. , Jazowiecka-Rakus J. , Szala S.
• Języki publikacji: EN

Abstrakty

EN

This report describes gene transfer in vitro as well as in vivo using cetylated low-molecular mass (600 Da) polyethylenimine (28% of amine groups substituted with cetyl moieties), termed CT-PEI. This compound is hydrophobic and has to be in­corporated into liposomes in order to be suitable for gene transfer studies. Serum-in­duced plasmid DNA degradation assay demonstrated that CT-PEI-containing lipo­somal carriers could protect complexed DNA (probably via condensation). In vitro lu- ciferase gene expression achieved using medium supplemented with 10% serum was comparable to that achieved in serum-reduced medium and was highest for CT-PEI/cholesterol liposomes, followed by CT-PEI/dioleoylphosphatidylcholine liposomes and PEI 600 Da (uncetylated) carrier. In vivo systemic transfer into mice was most efficient when liposome formulations contained CT-PEI and cholesterol. Higher luciferase expression was then observed in lungs than in liver. In conclusion: liposomes containing cetylated polyethylenimine and cholesterol are a suitable vehicle for investigating systemic plasmid DNA transfer into lungs.

Słowa kluczowe

EN

polyethylenimine; lipopolysaccharide; gene transfer; non-viral vector; cationic lipid; cetylated polyethylenimine; in vivo; polyethylene glycol

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2004
• Tom: 51
• Numer: 3
• Opis fizyczny: p.693-702,fig.,ref.

Twórcy

autor

Sochanik A.

• Maria Sklodowska-Curie Memorial Institute, 44-101 Gliwice, Poland

autor

Cichon T.

autor

Makselon M.

autor

Strozyk M.

autor

Smolarczyk R.

autor

Jazowiecka-Rakus J.

autor

Szala S.

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