Surface and ES antigens of adult Dicrocoelium dendriticum inducing bile antibody responses in naturally infected cattle

• Autorzy: Wedrychowicz H , Ducommun D , Klockiewicz M , Pfister K
• Języki publikacji: EN

Abstrakty

EN

Bile antibody responses of naturally infected cattle to surface and ES protein antigens of adult D. dendriticum were analysed. Freshly isolated and carefully purified flukes were surface labelled with NHS- biotin or biotin hydrazide (BH) and extracted using Tris-buffered saline. Biotinylated surface proteins or glycoproteins were then isolated by streptavidin affinity chromatography. ES products were obtained during 24 h incubation of undamaged worms at 37 °C in Tyrode’s salts solution enriched with antibiotics. Bile samples were collected at slaughter from cattle harbouring 120-280 lancet flukes. The fluke antigens inducing bile antibody responses were analysed by SDS-PAGE, Western blot and ELISA techniques. Bile from non-infected cattle precipitated none of the surface proteins and one polypeptide out of ES products. One to 6 polypeptides were found in immunocomplexes formed by individual bile samples of infected cattle and surface proteins while immunocomplexes formed by bile and surface extracts of flukes labelled with BH contained 2-5 fluke glycoproteins. An average of 3 polypeptides were recognised in ES products. In ELISA tests 38-67% out of 150 samples reacted with ES antigens while 70% possessed antibodies against surface proteins and 92% against surface glycoproteins. It is suggested that the surface and ES antigens may be of particular importance in the host-parasite relationship.

Słowa kluczowe

EN

surface protein; infection; sheep; Dicrocoelium dendriticum; excretory molecule; secretory product; antibody response; bile; parasite; secretory molecule; antigen; excretory product; cattle; surface glycoprotein; ELISA test; Fasciola hepatica

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 1996
• Tom: 41
• Numer: 3
• Opis fizyczny: p.139-144,fig.

Twórcy

autor

Wedrychowicz H

• Department of Parasitology, Warsaw Agricultural University, Grochowska 272, 03-849 Warsaw, Poland

autor

Ducommun D

• Labor Pfister, Morgenstr. 83B, Ch-3018 Bern, Switzerland

autor

Klockiewicz M

• Department of Parasitology, Warsaw Agricultural University, Grochowska 272, 03-849 Warsaw, Poland

autor

Pfister K

• Labor Pfister, Morgenstr. 83B, Ch-3018 Bern, Switzerland

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