Morphological, cytological and BSA-based testing on limited segregation population AFLPs

• Autorzy: Bednarek P T , Kubicka H. , Zawada M.
• Języki publikacji: EN

Abstrakty

EN

Cytoplasmic male sterility (cms) in rye (Secale cereale L.), especially cytoplasma PAMPA, is used commercially in hybrid beeding programmes. The development of molecular markers that are tightly linked to the numerous genes coding for pollen fertility is expected to have great impact in the field. Morphological and cytological analyses of plants from a three-way cross C394: [(S67P/94 x S38/94) x CHD296] indicated the presence of at least several genes acting at different stages of pollen grain development, and proved the concurrence of both approaches in plant classification. The AFLP technique combined with the Bulk Segregant Analysis (BSA) were applied to identify DNA fragments linked to the genes of interest. All the 256 possible primer pair combinations based on the Msel and EcoRI restriction sites generated distinct band patterns allowing the identification of 31143 DNA fragments, visualised using the isotopic method. On average, any given primer combination generated 122 fragments. Among 1111 and 431 potential genetic markers respectively identified in the restorer form and the maternal lines, 775 and 295 were present in the F2 population. These numbers were then reduced to 109 and 51. The identified DNA fragments were tested on a limited segregating population, C394-F2, in order to eliminate false signals and to select markers for a future marker-assisted selection programme. Twenty-five markers were selected. Four of these markers were not identified via the BSA approach, indicating that if a highly polymorphic component is used for a cross, or a polygenic trait is studied, then the use of a limited population may be required.

Słowa kluczowe

EN

pollen fertility; rye; Secale cereale; hybrid; morphology; cytology; breeding programme; molecular marker; cytoplasm; plant; cytoplasmic male sterility

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2002
• Tom: 07
• Numer: 2B
• Opis fizyczny: p.635-648,fig.

Twórcy

autor

Bednarek P T

• Polish Academy of Sciences, 02-793 Warsaw, Prawdziwka 2, Poland

autor

Kubicka H.

autor

Zawada M.

Bibliografia

• 1. Putt, E. D. Cytogenetic studies of sterility in rye. Can. J. Agricultural Sci. 34 (1954) 81-119.
• 2. Geiger, H. H. Cytoplasmatisch-genische Pollensterilitat in Roggenformen iranischer Abstammung. Die Naturwissenschaften 2 (1971) 98-99.
• 3. Kobyljanski, V. D. The production of sterile analogues of winter rye, sterility maintainers and fertility restorers. Tr. Prikl. Bot. Genet. Sel. 44 (1971) 76-84.
• 4. Kluchko, P. F. and Belousov, A. A. Genetic study of male sterility in winter rye. Genetika 8 (1972) 9-15.
• 5. Madej, L. Research on male sterility in rye. Hod. Rośl. Aklim. Nasienn. Warszawa 19 (1975) 421-422.
• 6. Geiger, H. H. and K. Morgenstern. Angewandt-genetische Studien zur cytoplasmatischen Pollenstarilitat bei Winterroggen. Theor. Appl. Genet. 46 (1975) 269-276.
• 7. Madej, L. The genetical characteristics of three sources of male sterility in rye (Secale cereale L.). Hod. Rośl. Aklim. Nasienn. Warszawa 20 (1976) 157-174.
• 8. Adolf, K. and Winkel, A. A new source of spontaneous sterility in winter rye. Preliminary results. In: Proc. Eucaria Meet. Cereal Sect. Rye. Svalov, Sweeden, (1985) 293-307.
• 9. Warzecha, R. and Salak-Warzecha, K. Comparitive studies on CMS sources in rye. Vortr. Pflanzenzuchtung 35 (1996) 39-49.
• 10. Łapiński, M. and Stojałowski, S. The C-source of sterility-inducing cytoplasm in rye: origin, identity, and occurrence. Vort. Pflanzenzuchtung 35 (1996) 51-60.
• 11. Geiger, H. H. and Schnell, F. W. Cytoplasmic male sterility in rye (Secale cereale L.). Crop Sci. 10 (1970) 590-593.
• 12. Garlocka, W. and Madej, L. Microsporogenesis and anther development in male-sterile and male-fertile forms of rye. Hod. Rośl. Aklim. Nasien. 19 (1975) 423-428.
• 13. Tudzynski, P., Rogmann, P. and Geiger, H. H. Molecular analysis of mitochondrial DNA from rye (Secale cereale L.). Theor. Appl. Genet. 72 (1986) 695-699.
• 14. Miedaner, T., Glass, C., Dreyer, F., Wilde, P., Wortmann, H. and Geiger, H. H. Mapping of genes for male-fertility restoration in ‘Pampa’ CMS winter rye (Secale cereale L.). Theor. Appl. Genet. 101 (2000) 1226-1233.
• 15. Bicar, E. H. and Darvey, N. L. Development of the components of a cytoplasmic male sterility hybrid system in rye through anther culture. Euphytica 97 (1997) 151-160.
• 16. Geiger, H. H. Breeding methods in diploid rye (Secale cereale L.). Tag.-Ber., Akad. Landwirtsch.-Wiss. DDR, Berlin 198 (1982) 305-332.
• 17. Geiger, H. H. Wiederschtellung der Pollenfertilitat in Roggenformen iranischer Herkunft. Theor. Appl. Genet. 42 (1972) 32-33.
• 18. Wricke, G., Wilde, P., Wehling, P. and Gieselmann, C. An isozyme marker for pollen fertility restoration to Pampa cms system of rye (Secale cerealae L.). Plant Breed. 111 (1993) 290-294.
• 19. Miedaner, T., Dreyer, P., Glass, C., Reinbold, H. and Geiger, H. H. Mapping of genes for pollen fertility restoration in rye (Secale cereale L.). Vortr. Pflanzenzuechtg 38 (1997) 303-314.
• 20. Dreyer, F., Miedaner, T. and Geiger, H. H. Chromosomal localization of male-fertility restorer genes from Argentinian and Iranian rye. Vortr. Pflanzenzuchtung 35 (1996) 280-281.
• 21. Scoles, G. J. and Evans, L. E. The genetics of fertility restoration in cytoplasmic male-sterile rye. Can. J. Genet. Cytol. 21 (1979) 417-422.
• 22. Bednarek, P. T., Kubicka, H., Zawada, M. and Brukwiński, W. Application of the AFLP markers for genetic variability testing within parental form of a rye hybrid based on the PAMPA cytoplasm. Biul. Inst. Hod. Aklim. Rośl. 211 (1999) 229-237.
• 23. Bednarek, P. T., Kubicka, H., Zawada, M. and Brukwiński, W. Bulk analysis of pseudo segregating F1 plants of rye hybrid based on cms PAMPA by the AFLP technique. Biul. Inst. Hod. Aklim. Rośl. 216 (2000) 101-110.
• 24. Vos, P., Hogers, R., Bleeker, M., Lee, T. van, Hornes, M., Frijters, A., Pot, J., Peleman, J., Kuiper, M. and Zauber, M. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res. 23 (1995) 4407-4414.
• 25. Michelmore, R. W., Paran, I. and Kesseli, R. V. Identification of markers linked to desease resistance genes by bulk segregant analysis: a rapid method to detect markers in specific genomic region by using segregating populations. Proc. Natl. Acad. Sci. USA 88 (1991) 9828-9832.
• 26. Bednarek, P. T., Chwedorzewska, K., Króliczak, J., Puchalski, J. and Zawada, M. AFLP molecular markers as a tool for genetic variability studies of rye inbred lines. Biul. Inst. Hod. Aklim. Rośl. 211 (1999) 219-227.
• 27. Yuan, Y. Umweltsensibilität der cytoplasmisch-genisch vererbten männlichen Sterilität (CMS) bei Roggen (Secale cereale L.). Dissertation, Univ. (1996) Hohenheim.
• 28. Morgenstern, K. Ausprägung der cytoplasmatisch-genischen Pollensterilität (CMS) bei Roggen in Abhängigkeit von Plasmotyp and Genotyp. Dissertation, Univ. (1983) Hohenheim.
• 29. Paran, I., Kesseli, R. V. and Michelmore, R. W. Identification of RFLP and RAPD markers linked to downy mildew resistance genes in lettuce, using nearly-isogenic lines. Genome 34 (1991) 1021-1027.
• 30. Ranade, S. A., Farooqui, N., Bhattacharya, E. and Verma, A. Gene tagging with random amplified polymorphic DNA (RAPD) markers for molecular breeding in plants. Crit. Rev. Plant Sci. 20 (2002) 251-275.
• 31. Wang, G. L. and Petreson, A. H. Assessment of DNA pooling strategies for mapping QTLs. Theor. Appl. Genet. 93 (1994) 920-925.
• 32. Lehner, A., Campbell, M. A., Wheeler, N. C., Poykko, T., Glossl, J., Kreike, J. and Neale, D. B. Identification of a RAPD marker linked to pendula gene in Norway spruce (Picea abies (L.) Karst. f. pendula). Theor. Appl. Genet. 91 (1995) 1092-1094.
• 33. Li, X., Eck, H. J. van, Voort, J. N. A. M. R. van, Huigen, D.-J., Stam, P., Jacobsen, E. Autotetraploids and genetic mapping using common AFLP markers: the R2 allele conferring resistance to Phytophthora infestans mapped on potato chromosome 4. Theor. Appl. Genet. 96 (1998) 1121-1128.
• 34. Senft, P. and. Wricke, G. An extended genetic map of rye (Secale cereale L.). Plant Breed. 115 (1996) 508-510.
• 35. Voylokov, A. V., Korzun, V. and Börner, A. Mapping of three self-fertility mutations in rye (Secale cereale L.) using RFLP, isozyme and morphological markers. Theor. Appl. Genet. 97 (1998) 147-153.
• 36. Börner, A. and Korzun, V. A consensus linkage map of rye (Secale cereale L.) including 374 RFLPs, 24 isozymes and 15 gene loci. Theor. Appl. Genet. 97 (1998) 1279-1288.
• 37. Ruebenbauer, T., Kubara-Szpunar, L and Pajak, K. Testing of the hypothesis concerning interaction of genes with mutated cytoplasm controlling male sterility of the Pampa type in rye (Secale cereale L.). Genetica Polonica 25 (1984) 1-15.
• 38. Voort, J. R. van, Wolters, P., Folkertsma, R., Hutten, R., Zandvoort, P. van, Vinke, H., Kanyuka, K., Bendahmane, A., Jacobsen, E., Janssen, R. and Bakker, J. Mapping of the cyst nematode resistance locus Gpa2 in potato using a strategy based on comigrating AFLP markers. Theor. Appl. Genet. 95 (1997) 874-880.
• 39. Voort, J. R. van, Zandvoort, P. van, Eck, H. J. van, Folkertsma, R. T., Hutten, R. C. B., Draaistra, J., Gommers, F. J., Jacobsen, E., Helder, J. and Bakker, J. Use of allele specificity of comigrating AFLP markers to align genetic maps from different potato genotypes. Mol. Gen. Genet. 255 (1997) 438-447.