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2004 | 46 | 4 |

Tytuł artykułu

Chlorophyll fluorimetry as a method for studying light absorption by photosynthetic pigments in marine algae

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Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Using laboratory cultures of algae and natural phytoplankton populations from Nhatrang Bay (South China Sea), the relationship between the chlorophyll fluorescence F0, the chlorophyll a concentration Ca and light absorption capacities of algae cells was studied. It is shown that the ratio F0/Ca depends mainly on the species composition of the algae population; hence, the concentration Ca can be measured with the fluorescence method with acceptable accuracy only when the species composition of algae populations varies over a rather narrow range. The fluorescence F0 can, however, be a good index of the total absorption capacities of different phytoplankton species, because the intensity of F0 depends on the sum total of light absorbed by all photosynthetic pigments in a plant cell. Thus, the fluorescence F0 measures not only the concentration of chlorophyll a, but that of all photosynthetic pigment concentrations.

Wydawca

-

Czasopismo

Rocznik

Tom

46

Numer

4

Opis fizyczny

p.519-531,fig.,ref.

Twórcy

autor
  • Moscow State University, Moscow, 119899 Russia
autor
autor
autor
autor

Bibliografia

  • Antal T.K., Venediktov P. S., Konev Yu. N., Matorin D. N., Hapter R., Rubin A.B., 1999, Assessment of vertical profiles of phytoplankton photosynthetic activity by the fluorescence method, Engl. Okeanologiya, 39 (2), 287–292.
  • Antal T.K., Venediktov P. S., Matorin D.N., Ostrowska M., Woźniak B., Rubin A. B., 2001, Measurement of phytoplankton photosynthesis rate using a pump-and-probe fluorometer, Oceanologia 43 (3), 291–313.
  • Cullen J. J., 1982, The deep chlorophyll maximum: comparing vertical profiles of chlorophyll a, Can. J. Fish. Aquat. Sci., 39, 791–803.
  • Dera J., 1995, Underwater irradiance as a factor affecting primary production, Diss. and monogr., Inst. Oceanol. PAS, Sopot, 7, 110.
  • Fadeev V. V., Filippova E. M., Maslov D.V., Matorin D.N., Venediktov P. S., 1999, Diagnostics of photosynthesising organisms by linear and non-linear fluorimetry, Proc. EOS/SPIE Int. Symp. ‘Envirosense’, 3821, 248–259.
  • Falkowski P.G., Raven J.A., 1997, Aquatic photosynthesis, Blackwell Sci., Oxford, 375 pp.
  • Ficek D ., 2001, Modelling the quantum yield of photosynthesis in various marine systems, Rozpr. i monogr., Inst. Oceanol. PAN, Sopot, 14, 224 pp., (in Polish).
  • Ficek D ., Ostrowska M., Kuzio M., Pogosyan S., 2000, Variability of the portion of functional PS2 reaction centres in the light of a fluorometric study, Oceanologia, 42 (2), 243–249.
  • Foy R.N., 1987, A comparison of chlorophyll a and carotenoid concentrations as indicators of algal, Freshwater Biol., 17 (2), 237–250.
  • Karabashev G. S., 1987, Fluorescence in the ocean, Gidrometeoizdat, Leningrad, 200 pp., (in Russian).
  • Klughammer C., 1992, Entwicklung und Anwendung neuer absorptionspectroskopischer Methoden zur Charakterisierung des photosynthetischen Elektronentransports in isolierten Chloroplasten und intakten Blättern, Ph.D. thesis, Würzburg Univ.
  • Kolber Z., Wiman K.D., Falkowski P.G., 1990, Natural variability in photosynthetic energy conversion efficiency: a field study in the Gulf of Maine, Limnol. Oceanogr., 35, 72–79.
  • Kolber Z., Zehr J., Falkowski P.G., 1988, Effects of growth irradiance and nitrogen limitation on photosynthetic energy conversion in photosystem II, Plant Physiol., 88, 72–79.
  • Lanskaya L.A., 1971, Growing of algae, [in:] Ecological physiology of sea planktonic algae, K.M. Kailov (ed.), Nauk. Dumka, Kiyev, 5–21.
  • Lorenzen C.A., 1966, A method for continuous measurement of in vivo concentration, Deep Sea Res., 13 (2), 223–227.
  • Majchrowski R., 2001, Influence of irradiance on the light absorption characteristics of marine phytoplankton, Stud. i rozpr., Pom. Akad. Pedagog., Słupsk, 1, 131 pp., (in Polish).
  • Majchrowski R., WoźniakB., Dera J., FicekD ., Kaczmarek S., Ostrowska M., Koblentz-Mishke O. I., 2000, Model of the ‘in vivo’ spectral absorption of algal pigments. Part 2. Practical applications of the model, Oceanologia, 42 (2), 191–202.
  • Margalef R., 1963, Modelos simplificados del ambiente marino para el estudio de la sucesión y distribución del fitoplancton y del valor indicador de sus pigmentos, Inv. Pesq., 23, 11–52.
  • Matorin D.N., Vavilin D.V, Konev Yu.N., Kazimirko Yu.V., Rubin A.B., 1997, A study on the correlation between pigment composition in microalgae and intensity of chlorophyll fluorescence measured with a pulse fluorometer, Mosc. Univ. Biol. Bull., 16 (1), 25–28.
  • Matorin D.N., Venediktov P. S., 1990, Chlorophyll luminescence in microalgae culture and natural phytoplankton populations, Itog. Nauk. Tek., Ser. Biofiz., 40, 40–101, (in Russian).
  • Matorin D.N., Venediktov P. S., Konev Yu.V., Rubin A. B., 1996, Application of a double-flash, impulse, submersible fluorimeter in the determination of photosynthetic activity of natural phytoplankton, Dokl. Russ. Acad. Sci.-Earth Sci. Sect., 350 (7), 1159–1161.
  • Mauzerall D., 1972, Light-induced fluorescence changes in Chlorella, and the primary photoreactions for the production of oxygen, Proc. Natl. Acad. Sci. USA., 69, 1358–1362.
  • Ostrowska M., 2001, Using the fluorometric method for marine photosynthesis investigations in the Baltic, Rozpr.i monogr., Inst. Oceanol. PAN, Sopot, 15, 194 pp., (in Polish).
  • Ostrowska M., Majchrowski R., Matorin D.N., Woźniak B., 2000a, Variability of the specific fluorescence of chlorophyll in the ocean. Part 1. Theory of classical ‘in situ’ chlorophyll fluorometry, Oceanologia, 42 (2), 203–219.
  • Ostrowska M., Matorin D.N., Ficek D., 2000b, Variability of the specific fluorescence of chlorophyll in the ocean. Part 2. Fluorometric method of chlorophyll a determination, Oceanologia, 42 (2), 221–229.
  • Parsons T.R., Strickland J.D.H., 1963, Discussion of spectrophotometric determination of marine plant pigments, with revised equations for ascertaining chlorophyll and carotenoids, J. Mar. Res., 21 (3), 155–163.
  • Sirenko L.A., 1988, Information value of chlorophyll content, Gidrobiol. Zh. [J. Hydrobiol.], 24 (4), 49–53.
  • Soo Hoo J. B., Kiefer D.A., Collins D. J., McDermid I. S., 1986, In-vivo fluorescence excitation and absorption spectra of marine phytoplankton. I. Taxonomic characteristics and responses to photoadaption, J. Plankton Res., 8, 197–214.
  • Tamiya H. K., Morimura K., Yokota M., Kunieda R., 1961, The mode of nuclear division in synchronous culture of Chlorella: comparison of various methods of synchronization, Plant Cell Physiol., 2, 383–403.
  • Vedernikov V. I., Vshyvsev V. S., Demidov A.A., Pogosian S. I., Sukhanova I.N., Fadeev V.V., Chekaluk A.M., 1990, Using fluorometric and photometric methods for chlorophyll a studying in the Black Sea in spring 1988, Okeanologiya, 30, 848–854, (in Russian).
  • Vinberg G.G., 1969, Primary production of water bodies, Russ. Acad., Sci. (Minsk), 348 pp., (in Russian).
  • Woźniak B., Dera J., Ficek D., Majchrowski R., Kaczmarek S., Ostrowska M., Koblentz-Mishke O. I., 1999, Modelling the influence of acclimation on the absorption properties of marine phytoplankton, Oceanologia, 41 (2), 187–210.
  • Woźniak B., Dera J., Ficek D., Majchrowski R., Kaczmarek S., Ostrowska M., Koblentz-Mishke O. I., 2000, Model of the ‘in vivo’ spectral absorption of algal pigments. Part 1. Mathematical apparatus, Oceanologia, 42 (2), 177–190.
  • Woźniak B. , Dera J., Ficek D., Majchrowski R., OstrowskaM., Kaczmarek S., 2003, Modelling light and photosynthesis in the marine environment, Oceanologia, 45 (2), 171–245.
  • Woźniak B., Dera J., Ficek D., Ostrowska M., Majchrowski R., Kaczmarek S., Kuzio M., 2002, The current bio-optical study of marine phytoplankton, Opt. Appl., 32 (1), 731–747.
  • Yentsch C. S., Menzel D.W., 1963, A method for the determination of phytoplankton, chlorophyll, and phaeophytin by fluorescence, Deep Sea Res., 10, 221–231.

Typ dokumentu

Bibliografia

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