A comparative CD and fluorescence study of a series of model calcium-binding peptides

• Autorzy: Goch G. , Kozlowska H. , Wojtowicz A. , Bierzynski A.
• Języki publikacji: EN

Abstrakty

EN

Lanthanide-saturated peptides analogous to calcium-binding loops of EF-hand proteins can be used to stabilize the α-helical structure of peptide or protein segments attached to their C-termini. To study conformational properties of such loop-containing hybrids it is necessary to produce them in bacteria. In peptides obtained in this way the helix will be destabilized by the negatively charged C-terminal α-carboxyl groups. We propose to blocke them by the homoserine lactone. The results presented in this paper indicate that the presence of the lactone even at the C-terminus of the loop does not have any negative effect on the loop helix-nucleation ability. On the other hand, the presence of the α-NH+3 at the loop N-terminus leads to a drop of metal-binding constant and loss of the rigid structure of the α-helical segment of the loop. The α-amino group separated by one glycine residue from the loop N-terminus should also be avoided because it perturbs the conformation of the N-terminal part of the loop and may reduce the loop affinity to lanthanide ions.

Słowa kluczowe

EN

fluorescence; calcium-binding peptide; protein; lanthanide-saturated peptide; alpha-helix

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1999
• Tom: 46
• Numer: 3
• Opis fizyczny: p.673-677,fig.

Twórcy

autor

Goch G.

• Polish Academy of Sciences, A.Pawinskiego 5A, 02-106 Warsaw, Poland

autor

Kozlowska H.

autor

Wojtowicz A.

autor

Bierzynski A.

Bibliografia

• 1. Siedlecka. M., Goch, G., Ejchart, A., Sticht. H. & Bierzyński, A. (1999) Proc. Natl. Acad. ScL U.S.A. 96, 903-908.
• 2. Dadlez, M., Góral, J. & Bierzyński, A. (1991) FEBS Lett. 282, 143-146.
• 3. Wójcik, J., Góral, J., Pawłowski, K. & Bierzyński, A. (1997) Biochemistry 36, 680-687.
• 4. Horrocks, J., W.DeW. & Sudnick, D.R. (1979) J. Am. Cherru Soc. 101, 334-340.