Wykrywanie toksynotworczosci szczepow Clostridium difficile przy pomocy szybkich metod diagnostycznych

• Autorzy: Pituch H. , Obuch-Woszczatynski P. , Rouyan G.S. , Martirosian G. , Meisel-Mikolajczyk F.
• Języki publikacji: PL

Abstrakty

PL

Przebadano próbki kału 53 pacjentów z biegunką poantybiotykową. Przy pomocy testów komercyjnych stwierdzono obecność toksyny A C. difficile w próbkach kału 16 pacjentów, co stanowiło 29,6%. Z 30 próbek wyhodowano szczepy C. difficile. Do dalszych badań użyto 25 szczepów. 3 z nich nie produkowały toksyny A in vitro. Wszystkie natomiast produkowały in vitro toksynę B. Analiza genetyczna przy pomocy reakcji łańcuchowej polimerazy wykazała obecność genów toksyny A i B.

EN

Feces of 53 patients from different hospital wards suffering from long term post-antibiotic therapy diarrhea were tested. For direct detection of C. difficile toxin A, in samples TCD (Becton-Dickinson), and C. difficile Toxin A Test (Oxoid) tests were used. Toxin A was detected in 16 samples (29,6% tested). C. difficile strains were isolated from 40% of the fecal samples. Toxin A was detected in 25 Clostridium difficile strains with commercial tests and toxin B was detected using McCoy cell line. Toxin A was not produced by 3 C. difficile strains in vitro, but toxin B was produced by all strains. The polymerase chain reaction (PCR) test showed that all isolated strains possess genes of toxins A and B.

Słowa kluczowe

PL

metody diagnostyczne; toksyny; szczepy bakteryjne; pacjenci; biegunki poantybiotykowe; Clostridium difficile; bakterie Gram-ujemne; toksynotworczosc; wykrywanie

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 1998
• Tom: 50
• Numer: 1-2
• Opis fizyczny: s.55-61,fot.,tab.,bibliogr.

Twórcy

autor

Pituch H.

• Akademia Medyczna, 02-004 Warszawa, ul.T.Chalubinskiego 5

autor

Obuch-Woszczatynski P.

autor

Rouyan G.S.

autor

Martirosian G.

autor

Meisel-Mikolajczyk F.

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