Digoxigenin-labelled molecular probe for the simultaneous detection of three potato pathogens: potato spindle tuber viroid [PSTVd], potato virus Y [PVY], and potato leafroll virus /PLRV/

• Autorzy: Welnicki M. , Zekanowski C. , Zagorski W.
• Języki publikacji: EN

Abstrakty

A molecular probe, p3POT, was constructed of PSTVd, PVY, PLRV cDNA fragments introduced into pUCl8 vector. Sequencing of the inserts revealed that cloned fragments covered conservative parts of pathogenic genomes. Dot-blot hybridization of digoxigenin-labelled construct to crude extracts from plants infected with different potato viruses proved high sensitivity and specificity of the p3POT probe. This makes p3POT probe an useful tool for the routine testing, and selection of virus-free potatoes

Słowa kluczowe

EN

cDNA probe; virus; potato leafroll virus; pathogen; potato spindle tuber viroid; potato virus Y; potato; dot-blot hybridization

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1994
• Tom: 41
• Numer: 4
• Opis fizyczny: p.473-475,fig.

Twórcy

autor

Welnicki M.

• Polish Academy of Sciences, A.Pawinskiego 5a, 02-106 Warsaw, Poland

autor

Zekanowski C.

autor

Zagorski W.

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