Identification of microsatellite markers in the rye genome

• Autorzy: Bolibok H , Rakoczy-Trojanowska M. , Wyrzykowska M. , Radecka M. , Orczyk W.
• Języki publikacji: EN

Abstrakty

EN

The rye genomic library, which consists of DNA fragments in the range of 0.5–1.1 kb, was screened for the presence of tri-and tetranucleotide and compound microsatellites. Of the 1,600,000 clones analysed, 102 clones were positive and 41 were suitable for SSR primer pair design. Twenty-six primer pairs amplified specific products, and six of them were capable of detecting polymorphism among 30 rye accessions of different genetic backgrounds. Using a set of Chinese Spring-Imperial wheat-rye addition lines, it was possible to locate 3 newly identified microsatellites on chromosomes 3R, 4R and 7R.

Słowa kluczowe

EN

DNA sequence; rye; clone; microsatellite; eukaryote; plant genetics; molecular map; genome; prokaryote; wheat-rye addition line; inbred line; clone number; DNA fragment

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2006
• Tom: 11
• Numer: 2
• Opis fizyczny: p.291-298,fig.,ref.

Twórcy

autor

Bolibok H

• Warsaw Agricultural University, Nowoursynowska 166, 02-787 Warsaw, Poland

autor

Rakoczy-Trojanowska M.

autor

Wyrzykowska M.

autor

Radecka M.

autor

Orczyk W.

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