Improvement of medium for Miscanthus x Giganteus callus induction and plant regeneration

• Autorzy: Plazek A , Dubert F.
• Języki publikacji: EN

Abstrakty

EN

Miscanthus × giganteus tissue cultures brown quickly and strongly, probably as an effect of oxidation of phenolics produced by plant tissue. The aim of the work was to improve the medium composition for callus induction and plant regeneration of M. giganteus, with two experiments. The first one was aimed at developing a protocol to inhibit biosynthesis and oxidation of phenolics. Callus was induced from immature inflorescences on basal MS medium with 6.5 mg · dm-3 2,4-D, 0.25 mg · dm-3 BAP, 500 mg · dm-3 casein hydrolysate and 30 g · dm-3 sucrose (control medium), and this medium supplemented with one of the following: 200 mg · dm-3 chitosan, 65 g · dm-3 banana pulp (BP), 100 mg · dm-3 cysteine, or 30 g · dm-3 honey instead of sucrose. Plant regeneration used basal MS medium supplemented with 30 g · dm-3 sucrose and 0.2 mg · dm-3 BAP or 0.05 mg · dm-3 KIN. The second experiment was to verify whether BP and honey increase callus production and plant regeneration. For callus induction the explants were put on the control medium and medium supplemented with one of these: 65 g · dm-3 BP, 30 g · dm-3 honey instead of sucrose, or 65 g · dm-3 BP + 30 g · dm-3 honey instead of sucrose. The regeneration medium was basal MS medium supplemented with 0.05 mg · dm-3 KIN and 30 g · dm-3 sucrose or 30 g · dm-3 honey. Tissue browning was independent of medium content, but there was less browning on medium supplemented with honey. In the first experiment, regenerated plants were obtained only on basal MS medium with 30 g · dm-3 sucrose and 0.05 mg · dm-3 KIN. In the second experiment, MS medium containing BP and honey instead of sucrose was the best medium for callus induction, and regeneration from these calli was highest on basal MS with KIN and honey.

Słowa kluczowe

EN

Miscanthus; Miscanthus x giganteus; banana plant; chitosan; cysteine; embryo-like structure; honey; phenolic acid; oxidation; regenerant

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2010
• Tom: 52
• Numer: 1
• Opis fizyczny: p.105-110,fig.,ref.

Twórcy

autor

Plazek A

• University of Agriculture, Podluzna 3, 30-239 Krakow, Poland

autor

Dubert F.

Bibliografia

• CHUGH S, GUHA S, and RAO U. 2009. Micropropagation of orchids: A review on the potential of different explants.Scientia Horticulturae 122: 507–520.
• CILLIERS JJL, and SINGLETON VL. 1990. Caffeic acid autooxidation and the effects of thiols. Journal of Agriculture andFood Chemistry 38: 1789–1796.
• DEBERGH P. 1994. In vitro culture of ornamentals. In: Vasil IK, Thorpe TA [eds.], Plant Cell and Tissue Culture,561–573, Kluwer Academic Publishers, Dordrecht.
• HOLME IB, KROGSTRUP P, and HANSEN J. 1997. Embryogenic callus formation, growth and regeneration in callusesand suspension culture of Miscanthus × ogiformis Honda 'Gigantheus' as affected by proline. Plant CellTissue Organ Culture 50: 203–210.
• HOŁDERNA-KĘDZIA E, and KĘDZIA B. 2006. Research on an antioxidant capacity of honeys. Acta Agrobotanica 59:265–269 (in Polish with English summary).
• KAHN V. 1985. Effects of proteins, protein hydrolyzates, and amino acids on o-dihydroxyphenolase activity of polyphenoloxidase of mushroom, avocado and banana. Journalof Food Science 50: 111–115.
• LEWANDOWSKI I. 2006. Miscanthus – a multifunctional biomass crop for the future. In: Jeżowski S, Wojciechowicz MK,Zenkteler E [eds.], Alternative Plants for Sustainable Agriculture.
• PAGEN, Centre of Excellence in Plant Agrobiology and Molecular Genetics, vol. 5: 83–90. Institute of Plant Genetics Polish Academy of Sciences, Poznań.
• LEWANDOWSKI I, and KAHNT G. 1993. Development of a tissue culture system with unemerged inflorescence ofMiscanthus 'Gigantheus' for the induction and regenerationof somatic emrbryoids. In: Schraudolf H, Vogel S,Weberling F [eds.], Beiträge zur Biologie der Pflanzen,439–451. Duncker and Humblot, Berlin.
• MURASHIGE T, and SKOOG F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures.Physiologia Plantarum 15: 473–497.
• NAIDU SL, and LONG SP. 2004. Potential mechanisms of lowtemperature tolerance of C4 photosynthesis inMiscanthus × giganteus: an in vivo analysis. Planta220: 145–155.
• PŁAŻEK A, DUBERT F, ŻUR I, and WALIGÓRSKI P. 2007. In vitro culture of Miscanthus × gigantheus. Zeszyty ProblemowePostępów Nauk Rolniczych 523: 175–184 (InPolish with English summary).
• SAPERS GM. 1992. Chitosan enhances control of enzymatic browning in apple and pear juice by filtration. Journal ofFood Science 57: 1192–1193.
• SEENI S, and LATHA PG. 2000. In vitro multiplication and ecorehabilitation of the endangered Blue Vanada. Plant Cell Tissue and Organ Culture 61: 1–8.
• SKRZYPEK E, and DUBERT F. 2002. Growth, differentiation and regeneration of field bean (Vicia faba var. minor) in in vitro cultures. Zeszyty Problemowe Postępów Nauk Rolniczych 488: 555–564 (In Polish with English summary).
• VANDER P, VARUM KM, DOMARD A, GUEDDARI NEE, and MOERSCHBACHER BM. 1998. Comparison of the ability of partiallyN-acetylated chitosan and chitooligosaccharides toelicit resistance in wheat leaves. Plant Physiology 118:1353–1359.
• WOJTASZEK P. 1997. Oxidative burst: an early plant response to pathogen infection. Biochemical Journal 322: 681–692.
• WALKER JRL. 1977. Enzymatic browning in foods. Its chemistry and control. Food Technology New Zeland 12:19–25.
• ZHANG D, and QUANTICK PC. 1997. Effects of chitosan coating on enzymatic browning and decay during postharveststorage of litchi (Litchi chinensis Sonn.) fruit.Postharvest Biology and Technology 12: 195–202.