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Tytuł artykułu

Oporne na metycyline szczepy Staphylococcus aureus izolowane od chorych w Panstwowym Szpitalu Klinicznym Nr 2 w Warszawie

Warianty tytułu

Języki publikacji

PL

Abstrakty

PL
Przedstawiono wyniki badań, których analiza pozwoliła na zidentyfikowanie dwóch, wieloopornych, epidemicznych szczepów MRSA w Państwowym Szpitalu Klinicznym Nr 2 w Szczecinie.
EN
Over a fivefold increase, from 11% to 58%, in the prevalence of methicillin-resistance was observed in 1994-95 amongst clinical isolates of Staphylococcus aureus in the State Clinical Hospital No 2 in Szczecin, one of the largest hospitals in the West Pomeranian region of Poland. The aim of this study was to see if any one particular strain was responsible for this apparent outbreak. Fifty-six randomly selected isolates were typed by SmaI macrorestriction analysis using PFGE and by analysis of antimicrobial susceptibility patterns. Results indicate the presence of two epidemic multi-drug resistant MRSA strains. Over 85% of typed MRSA belonged to the first strain, which was probably present in the hospital long before 1994. MRSA of this strain were isolated from patients in 8 hospital wards. The second strain was introduced into two wards of the hospital in the last year of the study.

Wydawca

-

Rocznik

Tom

52

Numer

3

Opis fizyczny

s.229-236,tab.,bibliogr.

Twórcy

autor
  • Akademia Medyczna, 70-111 Szczecin, ul.Powstancow Wielkopolskich 72
autor
autor

Bibliografia

  • 1. Ayliffe GAJ, Buckles A, Casewell MW i inni. Revised guidelines for the control of methicillin- resistant Staphylococcus aureus infection in hospitals. J Hosp Infect 1998; 39: 253-90.
  • 2. Bismuth R. Cocci a Gram positif et aminosides. W: L'Antibiogramme. Red. P. Courvalin, G Goldstein, A Philippon i J Sirot, MPC-Videom, Paris. 1985; 29-39.
  • 3. Working Party of the British Society for Antimicrobial Chemotherapy. A guide to sensitivity testing. J Antimicrob Chemother 1991; 27: Supl D. 1-55.
  • 4. Buu Hoi A. Cocci a Gram positif et macrolides-lincosamides-streptogramines. W: L'Antibiogramme. Red. P Courvalin, G Goldstein, A Philippon i J Sirot, MPC-Videom, Paris 1985; 41-8
  • 5. Report of the Comite de l'Antibiogramme de la Societe Francaise de Microbiologie. Statement 1966 CA-SFM. Zone sizes and MIC breakpoints for non-fastidiuos organisms. Clin Microbiol Infect 1996; 2 (S1): 46-49.
  • 6. deLencastre H, Figueiredo AMS, Urban C i inni Multiple mechanisms of methicillin resistance and improved methods for detection in clinical isolates of Staphylococcus aureus. Antimicrob Agents Chemoth 1991; 35: 632-39.
  • 7. Frenay HME, Theelen JPG, Schouls LM i inni. Discrimination of epidemic and nonepidemic methicillin-resistant Staphylococcus aureus strains on the basis of protein A gene polymorphism. J Clin Microbiol 1994; 32: 846-47.
  • 8. Goh SH, Byrne SK, Zhang JL, Chow AW. Molecular typing of Staphylococcus aureus on the basis of coagulase gene polymorphisms. J Clin Microbiol 1992; 30: 1642-45.
  • 9. Hiramatsu K, Aritaka N, Hanaki H i inni. Dissemination in Japanese hospitals of strains of Staphylococcus aureus heterogeneously resistant to vancomycin. Lancet 1997; 350: 1670-73.
  • 10. Hryniewicz W, Trzciński K, Krzysztoń-Russjan K, Gniadkowski M. Zasady doboru testów wrażliwości bakterii na antybiotyki i chemioterapeutyki. Mikrobiol Med 1999; 2: 12-28.
  • 11. Kloos WE, Bannerman TL. Staphylococcus and Micrococcus. W: Manual of Clin. Microbiol. Red.: PR Murray, EJ Baron, FC Tenover i RH Yolken. American Society for Microbiology, Washington D.C. 1992, 282-384.
  • 12. Łęski T, Oliveira D, Trzciński K i inni. Clonal distribution of methicillin-resistant Staphylococcus aureus in Poland. J Clin Microbiol 1998; 36: 3532-39.
  • 13. Murchan S, Trzciński K, Skoczyńska A i inni. Spread of old and new clones of epidemic methicillin-resistant Staphylococcus aureus in Poland. Clin Microbiol Infect 1998; 4: 481-90.
  • 14. NCCLS. Performance standard for antimicrobial disk susceptibility tests. Approved standard M2-A6. 1997; vol. 17. NCCLS, Villanova Pa.
  • 15. Tenover FC, Arbeit RD, Goering RV i inni. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel-electrophoresis - criteria for bacterial strain typing. J Clin Microbiol 1995; 33: 2233-39.
  • 16. Trzciński K, Cooper BS, Hryniewicz W, Dowson CG. Expression of the resistance to tetracyclines in strains of methicillin-resistant Staphylococcus aureus. J Antimicrob Chemoth 2000; 45: 763-70.
  • 17. Trzciński K, Hryniewicz W, Claus H, Witte W. Characterization of two different clusters of clonally related methicillin-resistant Staphylococcus aureus strains by conventional and molecular typing. J Hosp Infect 1994; 28: 113-26.
  • 18. Trzciński K, Hryniewicz W, Kluytmans J i inni. Simultaneous persistence of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus in a neonatal ward of a Warsaw Hospital. J Hosp Infect 1997; 36: 291-303.
  • 19. Trzciński K, vanLeeuwen W, vanBelkum A i inni. Two clones of methicillin-resistant Staphylococcus aureus in Poland. Clin Microbiol Infect 1997; 3: 198-207.
  • 20. vanBelkum A, vanLeeuwen W, Kaufmann M i inni. Assessment of resolution and intercenter reproducibility of results of genotyping Staphylococcus aureus by pulsed-field gel electrophoresis of Smal macrorestriction fragments: a multicenter study. J Clin Microbiol 1998; 36: 1653-9.

Typ dokumentu

Bibliografia

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