Regulation of protamine gene expression in an in vitro homologous system

• Autorzy: Jankowski J. M. , Cannon P.D. , Van der Hoorn F. , Wasilewska L.D. , Wong N.C.W. , Dixon G.H.
• Języki publikacji: EN

Abstrakty

EN

An in vitro transcription system from the trout testis nuclei was developed to study trout protamine gene expression. The protamine promoter contains, among others, two regulatory elements: 1) a cAMP-responsive element or CRE element (TGACGTCA) which is present in position 5' to TATA box, and 2) GC box (CCGCCC) which is present in position 3' to TATA box. The removal of the CRE-binding protein by titration (by the addition of appropriate oligonucleotides to the incubation mixture) resulted in a decrease in transcription of the protamine gene. These results were confirmed by experiments in which the pure CRE-binding factor (TPBP1) was used, as well as by those where a stimulatory effect of cAMP on protamine promoter transcription was observed. On the other hand, addition of oligonucleotides containing the GC-box sequence enhanced the protamine gene transcription indicating that the protein (Spl like) which binds to this sequence acts as a repressor of protamine gene expression. These results confirm the previously proposed model which suggested that the GC box played a role in negative regulation of the protamine gene expression. Involvement of some other factors in this process was also discussed.

Słowa kluczowe

EN

regulatory element; promoter; protamine gene; transcription; plasmid construction; in vitro; binding protein; nucleotide sequence; trout; Salmo gairdnerii

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1996
• Tom: 43
• Numer: 2
• Opis fizyczny: p.369-377,fig.

Twórcy

autor

Jankowski J. M.

• Polish Academy of Sciences, A.Pawinskiego 5a, 02-106 Warsaw, Poland

autor

Cannon P.D.

autor

Van der Hoorn F.

autor

Wasilewska L.D.

autor

Wong N.C.W.

autor

Dixon G.H.

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