Zastosowanie izolowanych komorek watroby do oceny toksycznego dzialania substancji chemicznych

• Autorzy: Zapor L , Skowron J. , Miranowicz-Dzierzawska K. , Golofit-Szymczak M. , Karlik W.
• Języki publikacji: PL

Abstrakty

PL

Na izolowanych hepatocytach w zawiesinie przeprowadzono ocenę cytotoksycznego działania etylobenzenu, n-heksanu i tetrachloroetylenu. Na podstawie oznaczania aktywności aminotransferazy asparaginowej w medium środowiskowym po inkubacji hepatocytów z badanymi związkami, dla każdego z nich obliczono wartość dawki CE50 którą następnie porównano z wartościami dawek LD50 uzyskanymi z badań na zwierzętach. Stwierdzono, że podobnie jak w doświadczeniach in vivo najsilniejsze działanie toksyczne wykazuje etylobenzen, następnie tetrachloroetylen i n-heksan.

EN

The cytotoxic activity of some organic solvents (tetrachloroethylene, ethylbenzene and n-hexane) was evaluated in vitro in frenshly isolated rat hepatocytes in suspension. The toxicity was assessed by the release of aspartate aminotransferase, a cytostolic enzym, to the cell culture medium. A quantitative difference was observed, and its extent depended on the concentration of the xenobiotics and the duration of the incubation period. Ethylbenzene was more cytotoxic than tetrachloroethylene and n-hexane. A comparison between the results of in vivo and in vitro studies indicates that the release of aspartate aminotransferase into the medium may be useful as a primary screening test using hepatocytes in suspension to detect toxicity of chemicals.

Słowa kluczowe

PL

aminotransferaza asparaginianowa; n-heksan; substancje chemiczne; etylobenzen; hodowla in vitro; tetrachloroetylen; toksycznosc

EN

aspartate aminotransferase; n-hexane; chemical substance; ethylbenzene; in vitro culture; tetrachloroethylene; toxicity

• Wydawca: -
• Czasopismo: Bromatologia i Chemia Toksykologiczna
• Rocznik: 1999
• Tom: 32
• Numer: 3
• Opis fizyczny: s.265-270,rys.,bibliogr.

Twórcy

autor

Zapor L

• Centralny Instytut Ochrony Pracy, 00-701 Warszawa, ul.Czerniakowska 16

autor

Skowron J.

autor

Miranowicz-Dzierzawska K.

autor

Golofit-Szymczak M.

autor

Karlik W.

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