Improved HPLC method for total plasma homocysteine detection and quantification

• Autorzy: Sawula W. , Banecka-Majkutewicz Z. , Kadzinski L. , Jakobkiewicz-Banecka J. , Wegrzyn G. , Nyka W. , Banecki B.
• Języki publikacji: EN

Abstrakty

EN

Recent clinical research has pointed at hyperhomocysteinemia as an independent risk factor in a number of cardiovascular and neurological diseases. We have improved a chromatographic method of total plasma homocysteine measurements in order to obtain higher sensitivity, reliability and reproducibility. The method demonstrates excellent linearity (R = 0.999), range (< 2-100 µM), precision (instrumental RSD 0.06 and method RSD 1.17), accuracy (recovery of 99.92 and RSD 1.27), reproducibility, quantification limit and ruggedness (e.g. pH from 2.0 to 2.5). Because even a small increase in homocysteine level can be a significant risk factor of cardiovascular diseases, such a precise method is required. The constructed method allows the measurement of plasma pyridoxal phosphate, PLP, the co-enzyme form of vitamin B6, on the same column and similar reagents. The developed method has been successfully applied to measure both total plasma and serum homocysteine in a group of acute stroke patients.

Słowa kluczowe

EN

quantification; homocysteine measurement; detection; stroke risk factor; validation; high performance liquid chromatography; plasma homocysteine

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2008
• Tom: 55
• Numer: 1
• Opis fizyczny: p.119-125,fig.,ref.

Twórcy

autor

Sawula W.

• University of Gdansk, Gdansk, Poland

autor

Banecka-Majkutewicz Z.

autor

Kadzinski L.

autor

Jakobkiewicz-Banecka J.

autor

Wegrzyn G.

autor

Nyka W.

autor

Banecki B.

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