Porownanie wlasnosci alfa-amylazy z larw trzeciego i czwartego stadium Anisakis simplex

• Autorzy: Lopienska E , Zoltowska K. , Rokicki J.
• Języki publikacji: PL

Abstrakty

EN

α-Amylase is present in the third (L3) and in the fourth-stage (L4) of larvae from Anisakis simplex. The enzymes from both sources differ in same of their properties. Α-Amylase from L3 showed a maximum at pH 7,8, enzyme from L4 stage at pH 6,5. The α-amylase from L3 was mainly lysosomal enzyme. The enzyme from L4 was located in the microsomal fraction. The L3 α-amylase showed the inhibition by EDT A and by -SH reagent iodoacetic acid. These agents did not change the activity of L4 enzyme. Bath izoenzymes were unaffected by calcium and magnesium ions. Generally the α-amylase from L4 stage had higher activity (3,71 u/mg) than L3 one (2,29 u/mg).

Słowa kluczowe

PL

badania porownawcze; Anisakis simplex; aktywnosc enzymatyczna; alfa-amylazy; parazytologia; pasozyty; stadia rozwojowe; larwy

• Wydawca: -
• Czasopismo: Wiadomości Parazytologiczne
• Rocznik: 2001
• Tom: 47
• Numer: 3
• Opis fizyczny: s.323-327,rys.,tab.,bibliogr.

Twórcy

autor

Lopienska E

• Uniwersytet Warminsko-Mazurski, ul.Zolnierska 14, 10-561 Olsztyn

autor

Zoltowska K.

autor

Rokicki J.

Bibliografia

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