Development of loop-mediated isothermal amplification [LAMP]-based SNP markers for shelf-life in melon [Cucumis melo L.]

• Autorzy: Fukuta S , Mizukami Y , Ishida A , Kanbe M
• Języki publikacji: EN

Abstrakty

EN

In this study, LAMP markers linked to shelf-life in melon (Cucumis melo L.) were developed by converting a cleaved amplified polymorphic sequences (CAPS) marker (C2). The CAPS-PCR fragments from the long-shelf-life melon (0-3) and short-shelf-life melon (Nat-2) were cloned and sequenced to construct LAMP primers. A single nucleotide polymorphism (SNP) was identified between 0-3 and Nat-2. LAMP primers were designed to detect the SNP. In the LAMP reaction to detect long-shelf-life melon, the turbidity of the templates using 0-3, F1, homozygous long-shelf-life F2 lines and heterozygous long-shelf-life F2 lines started to increase after 40 min. In contrast, the turbidity of Nat-2 and homozygous short-shelf-life F2 lines did not increase even after 90 min. In the LAMP reaction to detect short-shelf-life melon, the turbidity of the templates using Nat-2, F1, homozygous short-shelf-life F2 lines and heterozygous long-shelf-life F2 lines started to increase after 40 min. But the turbidity of 0-3 and homozygous long-shelf-life F2 lines did not increase after 90 min. This attests to the high reliability and usefulness of LAMP for marker-assisted selection.

Słowa kluczowe

EN

DNA level; muskmelon; breeding process; plant selection; loop-mediated isothermal amplification; melon; Cucumis melo; cleaved amplified polymorphic sequence marker; fruit; plant breeding

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2006
• Tom: 47
• Numer: 4
• Opis fizyczny: p.303-308,fig.,ref.

Twórcy

autor

Fukuta S

• Biotechnology Group, Aichi Pref. Agricultural Research Center, 1-1 Sagamine, Yazako, Nagakute, Aichi, 480-1193, Japan

autor

Mizukami Y

• Biotechnology Group, Aichi Pref. Agricultural Research Center, 1-1 Sagamine, Yazako, Nagakute, Aichi, 480-1193, Japan

autor

Ishida A

• Biotechnology Group, Aichi Pref. Agricultural Research Center, 1-1 Sagamine, Yazako, Nagakute, Aichi, 480-1193, Japan

autor

Kanbe M

• Biotechnology Group, Aichi Pref. Agricultural Research Center, 1-1 Sagamine, Yazako, Nagakute, Aichi, 480-1193, Japan

Bibliografia

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