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2001 | 47 | 3 |

Tytuł artykułu

Zwiekszenie swoistosci laboratoryjnej diagnostyki toksokarozy

Treść / Zawartość

Warianty tytułu

Języki publikacji

PL

Abstrakty

EN
The aim of the study was to enhance specificity of immunological diagnosis in toxocarosis. The investigations concem: detection of circulating parasite antigens using dot-ELISA technique and detection of specific IgG antigodies using ELISA test with low molecular weight (≤30 kD) excretory-secretory (ES) larval T. canis antigens. The circulating antigens were investigated in 60 sera and 20 anterior chamber fluids from children with ocular form of toxocarosis. These antigens were present only in 3 sera and 2 anterior chamber fluids in children with active intraocular inflamation and fluid antibodies titers 1:5120 and 1:20000. Comparative study with different ELISA tests indicated that use of low molecular weight antigens increase specificity of reaction.

Wydawca

-

Rocznik

Tom

47

Numer

3

Opis fizyczny

s.489-496,rys.,tab.,bibliogr.

Twórcy

  • Akademia Medyczna, ul.Wolska 37, 01-201 Warszawa

Bibliografia

  • BOWMAN D. D., MIKA-GRIEVE M., GRIEVE R. B. 1987. Circulating excretory-secretory antigen levels and specific antibody responses in mice infected with Toxocara canis. Am. J. Trop. Med. Hyg. 36: 75-82.
  • DZBENSKI T. H., BITKOWSKA E., GOŁĄB E. 1999. Detection of circulating antigen of Toxocara canis in the sera of naturally infected men and experimental animals. Acta Parasitol. 44: 137-141.
  • GENCHI C., FALAGIANI P., RIVA G., TINELLI M., BRUNELLO F., BOERO M., ALMAYIVA M. 1988. IgE and IgG antibodies in Toxocara canis infection. A clinical evaluation. Ann. Allergy. 61: 43-46.
  • JACQUIER P., GOTISTEIN B., STINGELIN Y., ECKERT J. 1991. Immunodiagnosis of toxocarosis in humans: evaluation of a new enzyme-linked immunosorbent assay kit. J. Clin. Microbiol. 29: 1831-35.
  • LUO Z. J., WANG G. X., YANG C.I., LUO C. H., CHENG S.W., LIAO L. 1999. Detection of circulating antigens and antibodies in Toxocara canis infection among children in Chengdu, China. J. Parasitol. 85: 252-256.
  • MAGNAVAL J. F., FABRE R., MAURIERES P., CHARLET J. P., DE LARRARD B. 1991. Application of the Western blotting procedure for the immunodiagnosis of human toxocariasis. Parasitol. Res. 77: 697-702.
  • NUNES C. M., TUNDISI R. N., GARCIA J.F., HEINEMANN M. B., OGASSAWARA S., RICHTZENHAIN J. 1997. Cross-reactions between Toxocara canis and Ascaris suum in the diagnosis of visceral larva migrans by western blotting technique. Rewista do Instituto de Medicina Tropical de Sao Paulo. 39: 253-256.
  • UHLIKOVA M., HUBNER J., KOLAVOVA L., POLACKOVA M. 1996. Immunological studies on human larval toxocarosis. Centr. Europ. J. Publ. Health. 4: 242-245.
  • ŻARNOWSKA H., JASTRZĘBSKA M. 1994. Excretory-secretory larval antigens of Toxocara canis: physico-chemical characteristics and specificity assayed by Western blot technique. Acta Parasitol. 39: 41-45.
  • ŻARNOWSKA H., BASIAK W., DZIUBEK Z. 1995. Specific and total serum IgE in ocular and visceral forms of human toxocarosis. Acta Parasitol. 40: 62-65.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-3d43c0e3-c0b9-4024-b8c6-2fe97e417b09
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