Glutamate exocrine dynamics augmented by plasma glutamine and the distribution of amino acid transporters of the rat pancreas

• Autorzy: Fukushima D. , Doi H. , Fukushima K. , Katsura K. , Ogawa N. , Sekiguchi S. , Fujimori K. , Sato A. , Satomi S. , Ishida K. , Fukushima K.
• Języki publikacji: EN

Abstrakty

EN

The nutritional and physiological roles of amino acid (AA)s have been investigated for individual organs. In the current study, we focused on the dynamics of glutamate and transport systems in the pancreas. We employed original procedures to obtain rat pancreatic juice (PJ) subjected to intravenous administration of alanyl-glutamine (AG) for AA analysis. The pancreatic expressions of the transporters were evaluated by immunohistochemistry. We found that glutamate was secreted into the PJ in the basal state. The intravenous administration of AG increased the concentration and total amount of glutamate excreted into the PJ. In terms of the transport systems, L-type AA transporter (LAT1) was identified exclusively in the islet cells. Glutamate transporter 1 (GLT1), glutamate-aspartate transporter (GLAST), vesicular glutamate transporter 1 (VGUT1) and cystine/glutamic acid transporter (xCT) were found in the islet cells. xCT was identified in the duct cells as well, but was not accompanied by the expression of 4F2 heavy chain (4F2hc) staining in the islets and the acinar cells, similar to neutral AA transporter (ASCT2) or b0,+-type AA transporter 1(BAT1). Excitatory AA transporter (EAAC) was identified only in the acinar cells. Glutamate was exclusively found in the acinar cells. We revealed the novel dynamics of glutamate in the rat PJ. The glutamate secretion into the PJ was augmented by plasma glutamine, indicating the de novo metabolisms of glutamate, together with the local expression of the related transporters.

Słowa kluczowe

EN

glutamate; transport system; pancreas; rat; pancreatic juice; intravenous administration; exocrine dynamics; plasma; glutamine; distribution; amino acid; immunohistochemistry

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2010
• Tom: 61
• Numer: 3
• Opis fizyczny: p.265-271,fig.,ref.

Twórcy

autor

Fukushima D.

• Tohoku University Hospital, 1-1 Seiryomachi, Aoba-ku, Sendai, 980-8574, Japan

autor

Doi H.

autor

Fukushima K.

autor

Katsura K.

autor

Ogawa N.

autor

Sekiguchi S.

autor

Fujimori K.

autor

Sato A.

autor

Satomi S.

autor

Ishida K.

autor

Fukushima K.

Bibliografia

• Deloar HM, Fujiwara T, Nakamura T, et al. Estimation of internal absorbed dose of L-[methyl-11C]methionine using whole-body positron emission tomography. Eur J Nucl Med 1998; 25: 629-633.
• Hustinx R, Lemaire C, Jerusalem G, et al. Whole-body tumor imaging using PET and 2-18F-fluoro-L-tyrosine: preliminary evaluation and comparison with 18F-FDG. J Nucl Med 2003; 44: 533-539.
• Sutinen E, Jyrkkio S, Varpula M, et al. Nodal staging of lymphoma with whole-body PET: comparison of [11C] methionine and FDG. J Nucl Med 2000; 41: 1980-1988.
• Van Venrooij WJ, Poort C, Kramer MF, Jansen MT. Relationship between extracellular amino acids and protein synthesis in vitro in the rat pancreas. Eur J Biochem 1972; 30: 427-433.
• Webster PD, Black O, Mainz DL, Singh M. Pancreatic acinar cell metabolism and function. Gastroenterology 1977; 73: 1434-1449.
• Fukushima K, Doi H, Satomi S. Free amino acids are secreted into pancreatic juice in the rat. JJSMN 2006; 40: 13-26.
• Yumikari K, Nakamura A. Seikagakujikkenkouza. Vol. 11. Tokyo, Tokyokagakudoujin Inc., 1977, pp. 1080-1087.
• Behar KL, Rothman DL. in vivo nuclear magnetic resonance studies of glutamate-gamma-aminobutyric acid-glutamine cycling in rodent and human cortex: the central role of glutamine. J Nutr 2001; 131(Suppl 9): 2498S-504S; discussion 523S-524S.
• Curi TC, De Melo MP, De Azevedo RB, Zorn TM, Curi R. Glutamine utilization by rat neutrophils: presence of phosphate-dependent glutaminase. Am J Physiol 1997; 273: C1124-C1129.
• Gstraunthaler G, Holcomb T, Feifel E, Liu W, Spitaler N, Curthoys NP. Differential expression and acid-base regulation of glutaminase mRNAs in gluconeogenic LLC-PK(1)-FBPase(+) cells. Am J Physiol Renal Physiol 2000; 278: F227-F237.
• Newsholme P, Procopio J, Lima MM, Pithon-Curi TC, Curi R. Glutamine and glutamate - their central role in cell metabolism and function. Cell Biochem Funct 2003; 21: 1-9.
• Reeds PJ, Burrin DG, Stoll B, Jahoor F. Intestinal glutamate metabolism. J Nutr 2000; 130(Suppl 4S): 978S-982S.
• Watford M, Chellaraj V, Ismat A, Brown P, Raman P. Hepatic glutamine metabolism. Nutrition 2002; 18: 301-303.
• Garlick PJ. The role of leucine in the regulation of protein metabolism. J Nutr 2005; 135(Suppl 6):1553S-1556S.
• Li C, Buettger C, Kwagh J, et al. A signaling role of glutamine in insulin secretion. J Biol Chem 2004; 279: 13393-13401.
• Newsholme P, Brennan L, Rubi B, Maechler P. New insights into amino acid metabolism, beta-cell function and diabetes. Clin Sci 2005; 108: 185-194.
• Zawalich WS, Yamazaki H, Zawalich KC, Cline G. Comparative effects of amino acids and glucose on insulin secretion from isolated rat or mouse islets. J Endocrinol 2004; 183: 309-319.
• Funakoshi A, Miyasaka K, Kitani K, et al. Comperative effects of mammalian pancreastatins on the pancreatic exocrine secretion. Jpn J Physiol 1989; 39: 901-905.
• Arii K, Kai T, Kokuba Y. Degradation kinetics of L-alanyl-L-glutamine and its derivatives in aqueous solution. Eur J Pharm Sci 1999; 7: 107-112.
• Furst P, Albers S, Stehle P. Availability of glutamine supplied intravenously as alanylglutamine. Metabolism 1989; 38(8 Suppl 1): 67-72.
• Beyreuther K, Biesalski HK, Fernstrom JD, et al. Consensus meeting: monosodium glutamate - an update. Eur J Clin Nutr 2007; 61: 304-313.
• Furst P, Pogan K, Stehle P. Glutamine dipeptides in clinical nutrition. Nutrition 1997; 13: 731-737.
• Kapica M, Puzio I, Kato I, Kuwahara A, Zabielski A. Role of feed-regulating peptides on pancreatic exocrine secretion J Physiol Pharmacol 2008, 59(Suppl 2): 145-159.
• Christensen HN. Role of amino acid transport and countertransport in nutrition and metabolism. Physiol Rev 1990; 70: 43-77.
• Kanai Y. Family of neutral and acidic amino acid transporters: molecular biology, physiology and medical implications. Curr Opin Cell Biol 1997; 9: 565-572.
• Kanai Y, Segawa H, Miyamoto K, Uchino H, Takeda E, Endou H. Expression cloning and characterization of a transporter for large neutral amino acids activated by the heavy chain of 4F2 antigen (CD98). J Biol Chem 1998; 273: 23629-23632.
• Chairoungdua A, Segawa H, Kim JY, et al. Identification of an amino acid transporter associated with the cystinuria-related type II membrane glycoprotein. J Biol Chem 1999; 274: 28845-28848.
• Kanai Y, Endou H. Heterodimeric amino acid transporters: molecular biology and pathological and pharmacological relevance. Curr Drug Metab 2001; 4: 339-354.
• Hisano S. Vesicular glutamate transporters in the brain. Anat Sci Int 2003; 78: 191-204.
• Sato H, Tamba M, Ishii T, Bannai S. Cloning and expression of a plasma membrane cystine/glutamate exchange transporter composed of two distinct proteins. J Biol Chem 1999; 274: 11455-11458.
• Kanai Y, Hediger MA. The glutamate and neutral amino acid transporter family: physiological and pharmacological implications. Eur J Pharmacol 2003; 479: 237-247.
• Howell JA, Matthews AD, Swanson KC, Harmon DL, Mattheus JC. Molecular identification of high-affinity glutamate transporters in sheep and cattle forestomach, intestine, liver, kidney, and pancreas. J Anim Sci 2001; 79: 1329-1336.
• von Schonfeld J, Goebell H, Muller MK. The islet-acinar axis of the pancreas. Int J Pancreatol 1994; 16: 131-140.
• Williams JA, Goldfine ID. The insulin-pancreatic acinar axis. Diabetes 1985; 34: 980-986.
• Furuta A, Noda M, Suzuki SO, et al. Translocation of glutamate transporter subtype excitatory amino acid carrier 1 protein in kainic acid-induced rat epilepsy. Am J Pathol 2003; 163: 779-787.
• Sewell WA, Young JA. Secretion of electrolytes by the pancreas of the anaestetized rat. J Physiol 1975; 252: 379-396.
• Bonner-Weir S, Toschi E, Inada A, et al. The pancreatic ductal epithelium serves as a potential pool of progenitor cells. Pediatr Diabetes 2004; 5(Suppl 2): 16-22.
• Rosenberg L. Induction of islet cell neogenesis in the adult pancreas: the partial duct obstruction model. Microsc Res Tech 1998; 43: 337-346.
• Izzat NN, Overturf M, Weisbrodt NW, Loose DD. Bile acid transport in hypercholesterolemic resistant rabbits. J Physiol Pharmacol 2009; 60: 79-84.
• Perriello G, Jorde R, Nurjhan N. Estimation of glucose-alanine-lactate-glutamine cycles in postabsorptive humans: role of skeletal muscle. Am J Physiol 1995; 269: E443-E450.