The present state of herbicide resistance of weed populations in the Czech Republic

• Autorzy: Salava J , Chodova D.

Warianty tytułu

PL

Obecny stan odpornosci na herbicydy populacji chwastow w Republice Czech

• Języki publikacji: EN

Abstrakty

EN

In 1985-2002 thirteen weeds resistant to atrazine were selected by a repeated application of triazine herbicides on arable land, in orchards, non-agricultural land and at railways in the Czech Republic. Recently Digitaria sanguinalis biotypes resistant to atrazine have been found at three railway junctions. Long-lasting application of the active ingredient imazapyr at railways caused selection of resistant Kochia scoparia biotypes. High resistance to chlorsulfuron has been discovered in five Apera spica-venti biotypes originating in winter cereals fields. The molecular basis of resistance to atrazine has been identified in the following weeds: Kochia scoparia, Solatium nigrum, Senecio vulgaris, Conyza canadensis, Digitaria sanguinalis, Amaranthus retroflexus and Chenopodium album. The resistance was conferred by a glycine for serine substitution at residue 264 of the D1 protein in all of those weeds. The resistance to imazapyr in Czech Kochia scoparia biotypes was conferred by a mutation at codon 574 of the ALS gene. Analysis of the results of DNA sequencing indicated, that the mutation induced a leucine for tryptophane substitution. There was excellent correspondence between the phenotypic resistance to herbicides of individual plants and the presence of mutations.

PL

W latach 1985-2002 wyselekcjonowano w Republice Czech 13 gatunków chwastów odpornych na atrazynę stosując powtarzające się zabiegi herbicydami triazynowymi na polach uprawnych, w sadach, na terenach nierolniczych i torach kolejowych. Ostatnio wykryto biotypy Digitaria sanguinalis odporne na atrazynę na trzech rozjazdach kolejowych. Długotrwałe stosowanie składnika aktywnego imazapyr na torach kolejowych spowodowało wyselekcjonowanie się odpornych biotypów Kochia scoparia. W pięciu biotypach Apera spica-venti pochodzących z upraw pszenicy wykryto wysoką odporność na chlorsulfuron. Molekularną bazę odporności na atrazynę określono u następujących gatunków chwastów: Kochia scoparia, Solanum nigrum, Senecio vulgaris, Conyza canadensis, Digitaria sanguinalis, Amaranthus retroflexus i Chenopodium album. Odporność była uwarunkowana zamianą seryny na glicynę w szczątkowym elemencie 264 białka Dl wszystkich wymienionych chwastów. Odporność na imazapyr w czeskich biotypach Kochia scoparia była uwarunkowana mutacją w kodonie 574 genu ALS. Analiza wyników sekwencjonowania DNA wskazywała, że w wyniku mutacji nastąpiło zastąpienie tryptofanu leucyną. W ramach fenotypowej odporności na herbicydy stwierdzono bardzo dobrą zgodność odporności u wszystkich gatunków roślin a obecnością mutacji.

Słowa kluczowe

EN

plant protection; herbicide resistance; plant population; weed; Czech Republic; triazine; psbA gene; mutation; polymerase chain reaction; sequencing; herbicide; plant species

• Wydawca: -
• Czasopismo: Journal of Plant Protection Research
• Rocznik: 2007
• Tom: 47
• Numer: 4
• Opis fizyczny: p.437-444,fig.,ref.

Twórcy

autor

Salava J

• Crop Research Institute, Drnovska 507, 161 06 Prague 6, Czech Republic

autor

Chodova D.

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