Gelsolin in human colon adenocarcinoma cells with different metastatic potential

• Autorzy: Litwin M. , Mazur A.J. , Nowak D. , Mannherz H.G. , Malicka-Blaszkiewicz M.
• Języki publikacji: EN

Abstrakty

EN

Gelsolin, one of a major actin-binding proteins, is involved in the regulation of actin cytoskeleton organization by its severing and capping activity towards actin filaments. Human colon adenocarcinoma cell line LS180 and its selected variants of different metastatic potential were used to check for a correlation between gelsolin level, its subcellular localization and the invasive capacity of cells. Based on immunoblotting experiments, a decreased level of gelsolin was detected in the most invasive 5W subline when compared to the parental cell line LS180. The intracellular distribution of actin filaments and gelsolin in colon adenocarcinoma cells was examined by confocal microscopy. In the 5W subline, unlike in the other examined cells, gelsolin was colocalized with filamentous actin at the cell periphery. In summary, in human colon adenocarcinoma cells, gelsolin level and its subcellular distribution seem to correlate with their metastatic potential.

Słowa kluczowe

EN

metastatic potential; adenocarcinoma cell; gelsolin; cancer; human disease; colon adenocarcinoma; gelsolin level; actin; subcellular distribution

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2009
• Tom: 56
• Numer: 4
• Opis fizyczny: p.739-743,fig.,ref.

Twórcy

autor

Litwin M.

• Polish Academy of Sciences, Pawinskiego, Warsaw, Poland

autor

Mazur A.J.

autor

Nowak D.

autor

Mannherz H.G.

autor

Malicka-Blaszkiewicz M.

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