Okreslanie sredniej masy molowej lipopolisacharydow paleczek Enterobacteriaceae metoda oznaczania kwasow tluszczowych przy uzyciu chromatografii gazowej

• Autorzy: Ziolkowski A. , Swierzko A.S. , Cedzynski M.
• Języki publikacji: PL

Abstrakty

PL

W biologicznych badaniach lipopolisacharydów (LPS), aktywność powszechnie wyrażana jest w jednostkach masy, bez uwzględnienia przeliczników molowych. Jest to szczególnie ważne przy porównywaniu preparatów różniących się długością łańcucha cukrowego i co się z tym wiąże, średnią zawartością lipidu A w cząsteczce, będącego aktywnym centrum endotoksyny. Zastosowanie ekwimolarnych dawek LPS pozwoliłoby na określenie wpływu zarówno wielkości jak i struktury części wielocukrowej na aktywność całego polimeru.

EN

In the paper, we propose a method for estimation of the mean molecular weight of lipopolysaccharide, which is important for accuracy of endotoxin activity investigation. In our study, it was assumed that lipid A portion in Enterobacterial lipopolysaccharide is substituted by four 3-hydroxytetradecanoic acid residues, Lipopolysaccharides of S, Ra, Rc and Re chemotypes being laboratory preparations as well as purchased from Sigma were investigated. Fatty acids were determined by of gas chromatography as methyl esters according to the procedure described by Wollenweber and Rietschel. Mean molecular weight was calculated by the formula: MMW = 4x10¬6 ng/mg NnM/mg . A high agreement between the estimated and the theoretical molecular weight values was demonstrated in the case of Salmonella minnesota R595 (Re) LPS preparation. As expected, LPS heterogeneity increase together with enlargement of polysaccharide chain lenght which is visible in electrophoregrams also. Except for LPS mean molecular weight estimation, the method allows its detection in various preparations and samples, distinguishing of R and S LPS forms as well as the determination of mean lenght of O-specific chain in lipopolysaccharides which structures are known.

Słowa kluczowe

PL

chromatografia gazowa; lipopolisacharydy; kwasy tluszczowe; bakterie Gram-ujemne; Enterobacteriaceae; oznaczanie; masa molowa

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 1998
• Tom: 50
• Numer: 3-4
• Opis fizyczny: s.215-222,fot.,rys.,tab.,bibliogr.

Twórcy

autor

Ziolkowski A.

• Centrum Mikrobiologii i Wirusologii PAN, 93-232 Lodz, ul.Lodowa 106

autor

Swierzko A.S.

autor

Cedzynski M.

Bibliografia

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