Identification of virulence genes in Campylobacter jejuni and C. coli isolates by PCR

• Autorzy: Wieczorek K , Osek J.
• Języki publikacji: EN

Abstrakty

EN

Using the PCR technique, the presence of the seven genotypie virulence markers of Campylobacter has been tested. It was found that all C. coli (92 strains) and C. jejuni (105 strains) possessed the flaA and cadF markers. Several strains were positive for the CDT toxin genes (76.6%, 85.3%, and 83.2% strains with the A, B, and C cdt gene variants, respectively). On the other hand, only 66.0% and 20.8% isolates had the iam and virB genes.

Słowa kluczowe

EN

polymerase chain reaction; detection; isolate; Campylobacter coli; Campylobacter jejuni; virulence marker

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2008
• Tom: 52
• Numer: 2
• Opis fizyczny: p.211-216,fig.,ref.

Twórcy

autor

Wieczorek K

• National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Osek J.

Bibliografia

• 1. Bacon D.J., Alm R.A., Burr D.H., Hu L., Kopecko D.J., Ewing C.P., Trust T.J., Guerry P.: Involvement of a plasmid in virulence of Campylobacter jejuni 81-176. Infect Immun 2000, 68, 4384-4390.
• 2. Bang D.D., Scheutz F., Ahrens P., Pedersen K., Blom J., Madsen M.: Prevalence of cytolethal distending toxin (cdt) genes and CDT production in Campylobacter spp. isolated from Danish broilers. J Med Microbiol 2001, 50, 1087- 1094.
• 3. Bereswill S., Kist M.: Recent developments in Campylobacter pathogenesis. Curr Opin Infect Dis 2003, 16, 487-491.
• 4. Black R.E., Levine M.M., Clements M.L., Hughes T.P., Blaser M.J.: Experimental Campylobacter jejuni infection in humans. J Infect Dis 1988, 157, 472-479.
• 5. CAMPYNET: http://campynet.vetinst.dk/Fla.htm
• 6. Carvalho A.C., Ruiz-Palacios G.M., Ramos-Cervantes P., Cervantes L.E., Jiang X., Pickering L.K.: Molecular characterization of invasive and noninvasive Campylobacter jejuni and Campylobacter coli isolates. J Clin Microbiol 2001, 39, 1353-1359.
• 7. Dassanayake R.P., Zhou Y., Hinkley S., Stryker C.J., Plauche G., Borda J.T., Sestak K., Duhamel G.E.: Characterization of cytolethal distending toxin of campylobacter species isolated from captive macaque monkeys. J Clin Microbiol 2005, 43, 641-649.
• 8. Datta S., Niwa H., Itoh K.: Prevalence of 11 pathogenic genes of Campylobacter jejuni by PCR in strains isolated from humans, poultry meat and broiler and bovine faeces. J Med Microbiol 2003, 52, 345-348.
• 9. Eyigor A., Dawson K.A., Langlois B.E., Pickett C.L.: Cytolethal distending toxin genes in Campylobacter jejuni and Campylobacter coli isolates: detection and analysis by PCR. J Clin Microbiol 1999, 37, 1646-1650.
• 10. Grodzik M., Sałamaszyńska A., Klimuszko D.: Mechanisms of pathogenicity of Campylobacter jejuni. Medycyna Wet 2005, 61, 1124-1127.
• 11. Hickey T.E., McVeigh A.L., Scott D.A., Michielutti R.E., Bixby A., Carroll S.A., Bourgeois A.L., Guerry P.: Campylobacter jejuni cytolethal distending toxin mediates release of interleukin-8 from intestinal epithelial cells. Infect Immun 2000, 68, 6535-6541.
• 12. Johnson W. M., Lior H.: A new heat-labile cytolethal distending toxin (CLDT) produced by Campylobacter spp. Microb Pathog 1988, 4, 115-126.
• 13. Konkel M.E., Garvis S.G., Tipton S.L., Anderson D.E. Jr, Cieplak W.: Identification and molecular cloning of a gene encoding a fibronectin-binding protein (CadF) from Campylobacter jejuni. Mol Microbiol 1997, 24, 953-963.
• 14. Konkel M.E., Gray S.A., Kim B.J., Garvis S.G., Yoon J.: Identification of the enteropathogens Campylobacter jejuni and Campylobacter coli based on the cadF virulence gene and its product. J Clin Microbiol 1999, 38, 510-517.
• 15. Korsak D., Dzierżanowska-Fangrat K., Popowski J., Rożynek E.: Prevalence of potential virulence markers iam in Campylobacter jejuni and Campylobacter coli isolates obtained from chicken carcasses. Rocz PZH, 2004, 55, 307-312.
• 16. Lara-Tejero M., Galan J.E: CdtA, CdtB, and CdtC form a tripartite complex that is required for cytolethal distending toxin activity. Infect Immun 2001, 69, 4358-4365.
• 17. Lara-Tejero M., Galan J.E: Cytolethal distending toxin: limited damage as a strategy to modulate cellular functions. Trends Microbiol 2002, 10, 147-152.
• 18. Louwen R.P., van Belkum A., Wagenaar J.A., Doorduyn Y., Achterberg R., Endtz H.P.: Lack of association between the presence of the pVir plasmid and bloody diarrhea in Campylobacter jejuni enteritis. J Clin Microbiol 2006, 44, 1867-1868.
• 19. Martinez I., Mateo E., Churruca E., Girbau C., Alonso R., Femandez-Astorga A.: Detection of cdtA, cdtB, and cdtC genes in Campylobacter jejuni by multiplex PCR. Int J Med Microbiol 2006, 296, 45-48.
• 20. Monteville M.R., Yoon J.E., Konkel M.E.: Maximal adherence and invasion of INT 407 cells by Campylobacter jejuni requires the CadF outer-membrane protein and microfilament reorganization. Microbiology 2003, 149, 153-165.
• 21. Pickett C.L., Pesci E.C., Cottle D.L., Russell G., Erdem A.N., Zeytin H.: Prevalence of cytolethal distending toxin production in Campylobacter jejuni and relatedness of Campylobacter sp. cdtB gene. Infect Immun 1996, 64, 2070-2078.
• 22. Pickett C.L., Whitehouse C.A.: The cytolethal distending toxin family. Trends Microbiol 1999, 7, 292-287.
• 23. Rozynek E., Dzierzanowska-Fangrat K., Jozwiak P., Popowski J., Korsak D., Dzierzanowska D.: Prevalence of potential virulence markers in Polish Campylobacter jejuni and Campylobacter coli isolates obtained from hospitalized children and from chicken carcasses. J Med Microbiol 2005, 54, 615-619.
• 24. Schmidt-Ott R., Pohl S., Burghard S., Weig M., Gross U.: Identification and characterization of a major subgroup of conjugative Campylobacter jejuni plasmids. J Infect 2005, 50, 12-21.
• 25. Snelling W.J., Matsuda M., Moore J.E., Dooley J.S.: Campylobacter jejuni. Lett Appl Microbiol 2005, 37, 297- 302.
• 26. Tracz D.M., Keelan M., Ahmed-Bentley J., Gibreel A., Kowalewska-Grochowska K., Taylor D.E.: pVir and bloody diarrhea in Campylobacter jejuni enteritis. Emerg Infect Dis 2005, 11, 838-843.
• 27. van Vliet A.H., Ketley J.M.: Pathogenesis of enteric Campylobacter infection. Symp Ser Soc Appl Microbiol 2001, 30, 45-56.
• 28. Whitehouse C.A., Balbo P.B., Pesci E.C., Cottle D.L., Mirabito P.M., Pickett C.L.: Campylobacter jejuni cytolethal distending toxin causes a G2-phase cell cycle block. Infect Immun 1998, 66, 1934-1940.
• 29. Wieczorek K., Osek J.: Multiplex PCR assays for simultaneous identification of Campylobacter jejuni and Campylobacter coli. Medycyna Wet 2005, 61, 797-799.
• 30. Ziprin R.L., Young C.R., Stanker L.H., Hume M.E., Konkel M.E.: The absence of cecal colonization of chicks by a mutant of Campylobacter jejuni not expressing bacterial fibronectin-binding protein. Avian Dis 1999, 43, 586-589.