An effect of the DGAT1 gene polymorphism on breeding value of Polish Holstein-Friesian sires

• Autorzy: Nowacka-Woszuk J , Noskowiak A. , Strabel T. , Jankowski T. , Switonski M.

Warianty tytułu

PL

Wplyw polimorfizmu genu DGAT1 na wartosc hodowlana buhajow rasy polskiej holsztynsko-fryzyjskiej

• Języki publikacji: EN

Abstrakty

EN

The effect of the GC→AA polymorphism in the DGAT1 gene causing amino acid substitution (łysinę – K to alanine – A) was estimated on the breeding value (BV) of 89 Polish Holstein-Friesian sires. A fragment of 411 bp of the DGAT1 gene was analysed by RFLP (Cfr1) or MSSCP (screening for other substitutions). Only GC→AA in exon 8 dinucleotide substitution was identified causing K232A amino acid substitution. The frequencies of K and A alleles were 0.54 and 0.46, respectively. The KK genotype (compared to AA and KA) was found associated with higher BV for fat and protein content of milk and lower BV for milk and protein yields. Distribution of the polymorphism within the Holstein-Friesian population, as well as its effect on bull BV are discussed.

PL

Celem pracy była analiza polimorfizmu K232A (substytucja lizyny alaniną) w białku DGAT1 oraz ocena wpływu tego polimorfizmu na wartość hodowlaną 89 buhajów rasy polskiej holsztyńskofryzyjskiej.Techniką PCR amplifikowano fragment 411 nukleotydów genu DGAT1, który następnie analizowano dwiema technikami: RFLP (z zastosowaniem enzymu restrykcyjnego CfrI) oraz MSSCP (dla poszukiwania innych substytucji). Zidentyfikowano tylko dinukleotydową substytucję GC→AA,wywołującą zmianę sekwencji aminokwasów (K232A). Frekwencja allelu K wyniosła 0,54, a allelu A – 0,46. Wykazano, że buhaje o genotypie KK mają wyższą wartość hodowlaną dla cech zawartości tłuszczu i białka, a niższą dla wydajności mleka i białka mleka niż buhaje AA i KA. Omówiono stopień rozprzestrzenienia badanego polimorfizmu w rasie holsztyńsko-fryzyjskiej oraz jego wpływ na wartość hodowlaną buhajów.

Słowa kluczowe

EN

animal breeding; DGAT1 gene; Holstein-Friesian breed; breeding value; cattle; milk production trait; sire; gene polymorphism

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2008
• Tom: 26
• Numer: 1
• Opis fizyczny: p.17-23,fig.,ref.

Twórcy

autor

Nowacka-Woszuk J

• Agricultural University of Poznan, Wolynska 33, 60-637 Poznan, Poland

autor

Noskowiak A.

autor

Strabel T.

autor

Jankowski T.

autor

Switonski M.

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