Differentiated pattern of protein composition of crystalline inclusions of newly isolated Bacillus thuringiensis strains from Silesia in Poland

• Autorzy: Guz K , Kucinska J. , Lonc E. , Doroszkiewicz W.
• Języki publikacji: EN

Abstrakty

EN

Protein profiles of crystal delta-endotoxins were determined in twenty nine Bacillus thuringiensis strains-soil and phylloplane isolates - from Poland. Electrophoretic analysis revealed quantatively and qualitatively different patterns of delta-endotoxin crystal preparations of these B. thuringiensis strains. The crystalline parasporal inclusions of B. thuringiensis isolates were composed of two, three, four or five proteins. Molecular weights of these polypeptides varied from 23.4 kDa to 142 kDa. There is lack of correlation between serovars of B. thuringiensis strains, the morphology of crystals and the number and size of proteins in parasporal inclusions.

Słowa kluczowe

EN

Silesian region; crystalline inclusion; protein composition; Poland; protein profile; Bacillus thuringiensis; crystal delta-endotoxin

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2005
• Tom: 54
• Numer: 4
• Opis fizyczny: p.263-269,fig.,ref.

Twórcy

autor

Guz K

• University of Wroclaw, 63 Przybyszewskiego street, 51-148 Wroclaw, Poland

autor

Kucinska J.

autor

Lonc E.

autor

Doroszkiewicz W.

Bibliografia

• Benintende G.B., J.E. Lopez-Meza, J.G. Cozzi, C.F. Piccinetti and J.E. Ibarra. 1997. Characterization of INTA 51-3, a new atypical strain of Bacillus thuringiensis from Argentina. Appl. Environ. Microbiol. 41: 396-401.
• Brizzard B.L. and H.R. Whiteley. 1988. Nucleotide sequence of an additional crystal protein gene cloned from Bacillus thuringiensis subsp. kurstaki. Nucleic Acids Res. 16: 2723-2723.
• Ceron J., A. Ortiz, R. Quintero, L. Guereca and A. Bravo. 1995. specific PCR primers directed to identify cryI and cryIII genes within a Bacillus thuringiensis strain collection. Appl. Environ. Microbiol. 61: 3826-3831.
• Chambers J.A., A. Jelen, M.P. Gilbert, C.S. Jany, T.B. Johnson and C. Gawron-Burke. 1991. Isolation and characterization of a novel insecticidal crystal protein gene from Bacillus thuringiensis subsp. aizawai. J. Bacteriol. 173: 3966-3976
• Cheong H. and S.S. Gill. 1997. Cloning and characterization of a cytotytic and mosquitocidal δ-endotoxin from Bacillus thuringiensis subsp. jegathesan. Appl. Environ. Microbiol. 63: 3254-3260.
• Choi S.K., B.S. Shin, E.M. Kong, H.M. Rho and S.H. Park. 2000. Cloning of a new Bacillus thuringiensis cry 11-type crystal protein gene. Curr. Microbiol. 41: 65-69.
• Crickmore N., D.R. Zeigler, J. Feitelson, E. Schnepf, J. Van Rie, D. Lereclus, J. Baum and D.H. Dean. 1998. Revision of the Nomenclature for the Bacillus thuringiensis Pesticidal Crystal Proteins. Microbiol. Mol. Biol. Rev. 62: 807-821.
• Delécluse A., M.-L. Rosso and A. Ragni. 1995. Cloning and Expression of a Novel Toxin Gene from Bacillus thuringiensis subsp. jegathesan Encoding a Highly Mosquitocidal Protein. Appl. Environ. Microbiol. 61: 4230-4235.
• Doroszkiewicz W. and E. Lonc. 1999. Biodiversity of Bacillus thuringiensis Strains in the Phylloplane and Soil of Lower Silesia Region (Poland). Acta Microbiol. Pol. 48: 355-361.
• Ellis R.T., B.A. Stockhoff, L. Stamp, H.E. Schnepf, G.E. Schwab, M. Knuth, J. Russell, G.A. Cardineau and K.E. Narva. 2002. Novel Bacillus thuringiensis binary insecticidal crystal proteins active on western corn rootworm, Diabrotica virgifera virgifera. Appl. Environ. Microbiol. 68: 1137-1145.
• Enwistle P.F., J.S. Cory, M.J. Bailey and S. Higgs. 1993. Bacillus thuringiensis, An environmental biopesticide: Theory and practice. John Wiley and Sons, Chichester, New York.
• Espinasse S., M. Gohar, J. Chafaux, C. Buisson, S. Perchat and V. Sane his. 2002. Correspondenne of high levels of beta-exotoxin I and the presence of crylB in Bacillus thuringiensis. Appl. Environ. Microbiol. 68: 4182-4186
• Gill S.S. and B.A. Federici. 1987. Cytolityc activity and immunological similarity of the Bacillus thuringiensis subsp. israelensis and B. thuringiensis subsp. morrisoni isolate PG-14. Appl. Environ. Microb. 53: 1251-1256
• Georghiou G.P. and M.C. Wirth. 1997. Influence of exposure to single versus multiple toxins of Bacillus thuringiensis subs, israelensis on development of resistance in the mosquito Culex quinquefasciatus (Diptera: Culicidae). Appl. Envirom. Microbiol. 63: 1095-1101.
• Honee G., T.P.M. van der Salm and B. Visser. 1988. Nucleotide sequence of crystal protein gene isolated from B. thuringiensis subspecies entomocidus 60.5 coding for a toxin highly active against Spodoptera species. Nucleic Acids. Res. 16: 6240-6240.
• Hongyu Z., Y. Ziniu and D. Wangxi. 2000. Composition and ecological distribution of Cry proteins and their genotypes of Bacillus thuringiensis isolates from Warehouses in China. J. Invert. Pathol. 79: 191-197.
• Höfte H. and H.R. Whiteley. 1989. Insecticidal crystal proteins of Bacillus thuringiensis. Microbiol. Rev. 53: 242-255.
• Kaelin P., P. Morel and F. Gadani. 1994. isolation of Bacillus thuringiensis from stored tabacco and Lasioderma serricorne (F.). Appl. Environ. Microbiol. 60: 19-25
• Kawalek M.D., S. Benjamin, H.I. Lee and S.S. Gill. 1995. Isolation and identification of novel toxins from Malaysia, Bacillus thuringiensis subsp. jegathesan. Appl. Environ. Microbiol. 61: 2965-2969
• Kim H.-S., S. Yamashita, T. Akao, H. Saitoh, K. Higuchi, Y.S. Park, E. Mizuki and M. Ohaba. 2000. In vitro cytotoxicity of non-Cyt inclusion proteins of a Bacillus thuringiensis isolate against human cells, including cancer cells. Lett. Appl. Microbiol. 89: 16-23.
• Koo B.T., S.H. Park, S.K. Choi, B.S. Shin, J.I. Kim and J.H. Yu. 1995. Cloning of a novel crystal protein gene cry IK from Bacillus thuringiensis subsp. morrisoni. FEMS Microbiol: Lett. 134: 159-164.
• Kuo W.-S., J.-H. Lin, C.-C. Tzeng, S.-S. Kao and K.-F. Chak. 2000. Cloning of two new cry genes from Bacillus thuringiensis subsp. wuhanensis strain. Curr. Microbiol. 40: 227-232.
• Laemmli U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 111: 680-685.
• Lee C.S. and A.I. Aronson. 1991. Cloning and analysis of delta-endotoxin genes from Bacillus thuringiensis subsp. alesti. J. Bacteriol. 173: 6635-6638.
• Lecadet M.M., E. Frachon, V.C. Dumanoir, H. Ripouteau, S. Hamon, P. Laurent and I. Thiery. 1999. Updating the H-antigen classification of Bacillus thuringiensis. J. Appl. Microbiol. 86: 660-672.
• Lonc E., W. Doroszkiewicz, M.J. Klowden, K. Rydzanicz and A. Galgan. 2001a. Entomopathogenic activities of environmental isolates of Bacillus thuringiensis against dipteran larvae. J. Vector Ecology 26: 15-20.
• Lonc E., J. Kucińska and K. Rydzanicz. 2001b. Toxicity isolates of Bacillus thuringiensis from Wroclaw against larvae of Aedes aegypti (in Polish). Wiad. Parazytol. 47: 297-303.
• Lonc E., J. Kucińska and K. Rydzanicz. 2003. Comparative Delta-endotoxins of Bacillus thuringiensis against Mosquito Vectors (Aedes aegypti and Culexpipiens). Acta Microbiol. Pol. 52: 293-300.
• Lonc E., M.-M. Lecadet, T.M. Lachowicz and E. Panek. 1997. Description of Bacillus thuringiensis wratislaviensis (H-47), a new serotype originating from Wroclaw (Poland), and other Bt isolates from the same area. Lett. Appl. Microbiol. 24:467-473.
• Lopez-Meza J.E. and J.E. Ibarra. 1996. Characterization of a novel strain of Bacillus thuringiensis. Appl. Environ. Microbiol. 62: 1306-1310.
• Martin P.A. and R.S. Travers. 1989. Worldwide abundance and distribution of Bacillus thuringiensis isolates. Appl Environ. Mircobiol. 55: 2437-2442.
• Nichols C.N., W. Ahmad and D.J. Ellar. 1989. Evidence fortwo different types of insecticidal P2 toxins with dual specifity in Bacillus thuringiensis subspecies. J. Bacteriol. 171: 5141-5147.
• Ohba M., H. Iwahana, S. Asano, N. Suzuki, R. Sato and H. Hori. 1992. A unique isolate of Bacillus thuringiensis serovar japonensis with a high larvacidal activity specific for scarabaeid beetles. Lett. Appl. Microbiol. 14: 54-57.
• Ohba M. and K. Aizawa. 1989. New flagellar (H) antigenic subfactors in Bacillus thuringiensis H serotype 3 with description of two new subspecies, Bacillus thuringiensis subsp. sumiyoshiensis (H3a: 3d) and Bacillus thuringiensis subsp. fukuokaenensis (H3a: 3d: 3e). J. Invert. Pathol. 54: 208-212.
• Ohba M. and K. Aizawa. 1986. Insect toxicity of Bacillus thuringiensis isolated from soil of Japan. J. Invert. Pathol. 47: 12-20.
• Ragni A., I. Thiery and A. Delecluse. 1996. Characterization of six highly mosquitocidal Bacillus thuringiensis strains that do not belong to H-14 serotype. Curr. Microbiol. 32: 48-54.
• Saitoh H., K. Higuchi, E. Mizuki, S.H. Hwang and M. Ohba. 1998. characterization of mosquito larvacidal parasporal inclusions of a Bacillus thuringiensis serovar higo strain. J. Appl. Microbiol. 84: 883-888.
• Shin B.S., S.H. Park, S.K. Choi, B.T. Koo, S.T. Lee and J.I. Kim. 1995. Distribution of cry-V type insecticidal protein genes in Bacillus thuringiensis subsp. kurstaki and Bacillus thuringiensis subsp. entomocidus. Appl. Environ. Microbiol. 61: 2402-2407.
• Święcicka I. and I. Mahillon. 2005. The clonal structure of Bacillus thuringiensis isolates from north-east Poland does not correlate with their cry gene diversity. Environ. Microbiol. 7: 34-39.
• Tabashnik B.E. 1992. Evaluation of synergism among Bacillus thuringiensis toxins. Appl. Environ. Microbiol. 58: 3343-3346.
• Tailor R., J. Tippett, G. Gibb, S. Pells, D. Pike, L. Jordan and S. Ely. 1992. Identification and characterization of a novel Bacillus thuringiensis delta-endotoxin entomocidal to coleopteran and lepidopteran larvae. Mol. Microbiol. 6: 1211-1217.
• Thomas WE. and D.J. Ellar. 1983. Bacillus thuringiensisvar israelens is crystal delta-endotoxin: effects on insect and mammalian cells in vitro and in vivo. J. Cell Sci. 60: 181-197.
• Wasano N., H. Saito and M. Ohba. 1997. A high homology exists in N-terminal amino acid sequences of delta-endotoxins between Lepidoptera-specific and Coleoptera-specific Bacillus thuringiensis strains. Lett. Appl. Microbiol. 24: 438-440.
• Wirth M.C., B.A. Federici and W.E. Walton. 2000. Cyt 1A from Bacillus thuringiensis synergize activity of Bacillus sphaericus against Aedes aegypti (Diptera: Culicidae). Appl. Environ. Microbiol. 66: 1093-1097.