Antagonistyczne dzialanie bakterii z rodzaju Lactobacillus wobec beztlenowych i mikroaerofilnych czynnikow zakazen przewodu pokarmowego [Helicobacter pylori, Campylobacter coli, Campylobacter jejuni, Clostridium difficile]

• Autorzy: Strus M. , Pakosz K. , Gosciniak H. , Przondo-Mordarska A. , Rozynek E. , Pituch H. , Meisel-Mikolajczyk F. , Heczko P.B.
• Języki publikacji: PL

Abstrakty

PL

Określono antagonistyczne właściwości bakterii z rodzaju Lactobacillus, wyizolowanych z kału zdrowych noworodków karmionych mlekiem matki, wobec najczęstszych beztlenowych i mikroaerofilnych etiologicznych czynników zakażeń przewodu pokarmowego. Stwierdzono, że większość z przebadanych szczepów Lactobacillus w warunkach in vitro wykazuje wyraźne antagonistyczne działanie wobec badanych szczepów wskaźnikowych tj. Helicobacter pylori, Campylobacter coli, Campylobacter jejuni i Clostridium difficile.

EN

Antagonistic activity of Lactobacillus strains has been known for some time. This property is connected with production of many active substances by lactobacilli e.g., organic acids and bacteriocin-like substances which interfere with other indigenous microorganisms inhabiting the same ecological niche, including also anaerobic gastrointestinal tract pathogens. Growing interest of clinical medicine in finding new approaches to treatment and prevention of common inflammatory infections of the digestive tract resulted in studies on a possible usage of lactic acid bacteria. Last years, several in vitro and in vivo experiments on antagonism of different Lactobacillus strains against Helicobacter pylori and Clostridium difficile were performed. These observations had been done on already established, well known probiotic Lactobacillus strains. We tested antibacterial activities of Lactobacillus strains isolated from human digestive tract. As indicator bacteria, four species known as anaerobic bacterial etiologic agents of gastroenteric infections: Helicobacter pylori, Campylobacter jejuni, C. coli and Clostridium difficile were used. Some of them were obtained from international collections, others were clinical isolates from specimens taken from patients with different defined gastrointestinal infections. We used a slab method of testing inhibitory activity described in details previously. Following conclusions were drawn from our study: - All tested human Lactobacillus strains were able to inhibit the growth of all strains of anaerobic human gastrointestinal pathogens used in this study. - Inhibitory activities of tested Lactobacillus strains against Helicobacter pylori, Campylobacter spp., and Clostridium difficile as measured by comparing mean diameters of the inhibition zones were similar. - Differences in susceptibility of individual indicator strains of Campylobacter spp. and Clostridium difficile to inhibitory activity of Lactobacillus strains were small. - A similar mechanism of inhibition of anaerobic bacteria by lactobacilli is postulated.

Słowa kluczowe

PL

dzialanie antagonistyczne; zakazenia przewodu pokarmowego; choroby czlowieka; Lactobacillus; Clostridium difficile; Campylobacter coli; bakterie; Campylobacter jejuni; Helicobacter pylori

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2001
• Tom: 53
• Numer: 2
• Opis fizyczny: s.133-142,rys.,tab.,bibliogr.

Twórcy

autor

Strus M.

• Uniwersytet Jagiellonski, 31-121 Krakow, ul.Czysta 18

autor

Pakosz K.

autor

Gosciniak H.

autor

Przondo-Mordarska A.

autor

Rozynek E.

autor

Pituch H.

autor

Meisel-Mikolajczyk F.

autor

Heczko P.B.

Bibliografia

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