DNA polymorphism of the alphaA-globin gene in domestic pigeon

• Autorzy: Dybus A , Chang M.-H. , Cheng Y.-H. , Szatkowska I.

Warianty tytułu

PL

Polimorfizm DNA w genie alfaA-globiny golebia domowego

• Języki publikacji: EN

Abstrakty

EN

The study aimed at identifying the polymorphism of domestic pigeon αA-globin gene which can be a potential homing marker in selection of racing pigeons. A total of 329 domestic pigeons were genotyped using the polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP)method. The PCR products were digested with 17 restriction endonucleases. One RFLP – detected with HphI – was found in intron 1 and represented a C/T mutation. The second RFLP – detected with BseLI – was a point mutation in the 3’UTR region of the gene (C/T mutation). The polymorphism in the 3’UTR region of the pigeon αA-globin gene can potentially affect the stability of mRNA and modify the gene expression. The mechanisms of haemoglobin function reflecting variants of the αA-globin gene remain unknown.

PL

Celem pracy była identyfikacja polimorfizmu w genie αA-globiny gołębia domowego, który może być rozpatrywany jako potencjalny marker dla cech wydolności powrotnolotnej w selekcji gołębi pocztowych (sportowych). Badaniami objęto 329 gołębi domowych, wykorzystano technikę PCRRFLP.W poszukiwaniu zmienności sekwencji nukleotydowej zastosowano 17 enzymów restrykcyjnych.Zidentyfikowano dwa miejsca polimorficzne: pierwsze dla enzymu HphI zlokalizowane w 1 intronie genu – substytucja C/T – oraz drugie dla BseLI w rejonie 3’UTR genu (C/T). Polimorfizm w rejonie 3’UTR genu αA-globiny gołębia może potencjalnie wpływć na stabilność mRNA, modyfikując przez to ekspresję genu. Wpływ opisanej mutacji w rejonie 3’UTR na funkcje hemoglobiny pozostaje nieznany.

Słowa kluczowe

EN

domestic pigeon; DNA polymorphism; genetic marker; pigeon; polymorphism; bird; globin gene

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2008
• Tom: 26
• Numer: 3
• Opis fizyczny: p.219-226,fig.,ref.

Twórcy

autor

Dybus A

• Agricultural University of Szczecin, 71-460 Szczecin, Poland

autor

Chang M.-H.

autor

Cheng Y.-H.

autor

Szatkowska I.

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