Inhibition of [Na a. K]-ATPase by organophosphorus insecticides

• Autorzy: Blasiak J.
• Języki publikacji: EN

Abstrakty

EN

The organophosphorus insecticide bromfenvinios and its methylated homologue methylbrom- fenvinfos inhibited the activity of pig kidney (Na++K+)-ATPase contained in the microsomal fraction and purified from it. The effect was dose-dependent Subrate kinetic studies of the enzyme revealed the existence of two active sites with high and low affinity to ATP respectively. The Dixon analysis of the mode of inhibition indicated its noncompetitive character. The inhibition was more pronouced for bromfenvinfos than for methylbromfenvinfos and the purified enzyme was more affected than the enzyme contained in the microsomal fraction. The Hill plot of inhibition indicated a multisite binding of both insecticides exhibiting cooperativity in binding. The Hill coefficient (n) fulfilled the relationship 1 < n < 3. These properties of the interaction suggest an allosteric nature of the inhibitory action of the insecticides. An indirect mechanism of the interaction was proposed: methylparathion could inhibit the activity of the (Na+ + K+)-ATPase by excluding the enzyme protein from its normal lipid milieu.

Słowa kluczowe

EN

pig; enzyme; lipid-protein interaction; organophosphorus insecticide; allosteric inhibition; phospholipid; sodium; lipid; ATPase; potassium; protein; kidney; cholesterol; inhibitor

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 1995
• Tom: 04
• Numer: 1
• Opis fizyczny: p.23-26,fig.

Twórcy

autor

Blasiak J.

• University of Lodz, ul.Banacha 12-16, 90-237 Lodz, Poland

Bibliografia

• BRUGEL W. Fundamentals of the theory of infrared spectra in the gaseous state. An introduction to infrared spectroscopy (Brugel W., ed.). Methuen & Co. Ltd.: London, John Wiley & Sons, Inc.: New York, pp. 11-92, 1962.
• KENDALL D.N. Infrared radiation: description and simple theory of absorption by molecules. Applied infrared spectroscopy (Kendall D.N., ed.). Reinhold Publishing Corporation: New York, pp. 1-30,1966.
• KENDALL D.N. Survey of practical information. Applied infrared spectroscopy (Kendall D.N., ed.) Reinhold Publishing Corporation: New York, pp. 31-87, 1966.
• WOJTKOWIAK B„ CHABANEL M. Spektrochemia molekularna. PWN: Warszawa, pp. 114-145, 1984.
• KIJCKI Z. Podstawy spektroskopii molekularnej. PWN: Warszawa, pp. 11-161, 1992.
• PLYLER E.K., BARKER E.F. The infrared spectrum and the molecular configuration of N20. Phys. Rev., 38, 1827-1836, 1931.
• BARKER E.F. Constants of the NaO molecule. Phys. Rev., 41, 369-370, 1932.
• RICHARDSON W.S., WILSON E.B. Jr., The infra-red spectrum of N15N140 and the force constants of nitrous oxide. J. Chem. Phys., 18, 694-696, 1950.
• HERZBERG G., HERZBERG L. Rotation-vibration spectra of diatomic and simple polyatomic molecules with long absorption paths. VI. The spectrum of nitrous oxide (N20) below 1.2 p J.Chem. Phys., 18, 1551-1561, 1950.
• DOUGLAS A.E., MOLLER C.K. The near infrared spectrum and the interamolecular distances of nitrous oxide. J.Chem. Phys., 22, 275-279,1954.
• BEGUN G.M., FLETCHER W.H. Infrared spectra of the isotopic nitrous oxides. J.Chem. Phys., 28, 414-418, 1958.
• PLIVA J. Infrared spectra of isotopic nitrous oxides. J. Mol. Spectrosc., 12, 360-386, 1961.
• GROSSO R.P., MCCUBBIN T.K. Jr., The v,-2v2 Fermi diads of N24016, N15N14016, and N15016. J. Mol. Spectrosc., 13, 240-255, 1964.
• LACOME N., LEVY A. A parametric deconvolution method: application to two bands of N20 in the 1.9-pm region. J. Mol. Spectrosc., 71,175-192,1978.
• NAGAI K., KAWAGUCHI K, YAMADA C., HAYAKAWA K., TAKAGI T., HIROTA E. A high-precision wavelength meter for tunable diode laser. Measurement of C02 and NaO bands at 10 pm. J. Mol. Spectrosc., 84, 197-203,1980.
• JOLMA K, KAUPPINEN J., HORNEMAN V.-M. Vibration-rotation spectrum of N20 in the region of the lowest fundamental v2. J. Mol. Spectrosc., 101, 278-284, 1983.
• POLLOCK C.R., PETERSEN F.R., JENNINGS D.A., WELLS J.S. Absolute frequency measurements of the 00°2 - 00°0, 20°1 —00°0, and 12°l-00°0 bands of N20 by heterodyne spectroscopy. J.Mol. Spectrosc., 107, 62-71, 1984.
• VANEK M.D., JENNINGS D.A., WELLS J.S., MAKI A.G. Frequency measurements of high-J rotational transitions of OCS and NaO. J. Mol. Spectrosc., 138, 79-83, 1989.
• MAKI A.G., WELLS J.S., VANEK M.D. Heterodyne fraquency measurements on N20 near 930 cm-1. J. Mol. Spectrosc., 138, 84-88, 1989.
• WALLACE L., LIVINGSTON W. Spectroscopic observations of atmospheric trace gases over Kitt Peak. 2. Nitrous oxide and carbon monoxide from 1979 to 1985. J. Geophys. Res. 95, 16383-16390,1990.
• BALDACCHINI G., CHAKRABORII P.K., D’AMATO F. Lineshape in a Laval molecular beam and pressure broadening of nitrous oxide transition lines. J. Quant. Spectrosc. Radiat. Transfer, 49, 439-447, 1993.
• TAN T.L., LOOI E.C., LUA K.T. Hot-band spectrum near 589 cm"1. J. Molec. Spectrosc., 154, 218-222, 1992.
• YANG X., PETRILLO C.J., NODA C. Photoacoustic detection of N20 and C02 overtone transitions in the near-infrared. Chem. Phys. Lett., 214, 536-540,1993.
• BORELLO E., CERRUTI L, GHIOTTI G., GUGLLELLMINOTII E. Surface copmlexes of nitrous oxide adsorbed on a —Cr203. Inorg. Chim. Acta, 6, 4548, 1972.
• ALEKSEEV A.V., POZDNYAKOV D.V., TSYGANINKO A.A, FILIMONOV V.N. Infrared study of photooxidation of nitrogen oxides and ammonia adsorbed on alumina. React. Kinet. Catal. Lett., 5, 9-14, 1976.
• DOLPHIN D., WICK A. Tabulation of infrared spectral data. John Wiley & Sons: New York-London-Sydney- -Toronto, p. 487,1977.
• MORRTERRA C, BOCCUZZI F, COLUCCIA S., GHIOTTI G. Infrared study of the adsorption of nitrous oxide on ^-alumina. J. Catal., 65, 231-234, 1980.
• HUSSAIN G, RAHMAN M.M., SHEPPARD N, An infrared study of adsorption of N20 on ZnO. Spectrochim. Acta, 47A, 1525-1530, 1991.
• GARRONE E, UGLIENGO P., GHIOTTI G., BORELLO E. Ab inito and experimental study of the interaction of nitrous oxide with the isolated hydroxyl of silica. Spectrochim. Acta. 49A, 1221-1234, 1993.
• BIGELEISEN J., FRIEDMAN L. The infra-red spectra of NI5N15016 and NI4N15N16. Some thermodynamic properties of the isotopic NaO molecules. J. Chem. Phys., 18, 1656-1659, 1950.
• THOMPSON H.W., WILLIAMS R.L. Vibration bands and molecular rotational constants of nitrous oxide. Proc. Roy. Soc. (London), A220, 435-445, 1953.
• CHRISTENSEN M.T., THOMSON H.W. The rotational constants of nitrous oxide. Trans Faraday Soc., 50, 1027-1030, 1954.
• PLYER E.K., TIDWELL E.D., ALLEN H.C, Jr. Near infrared spectrum of nitrous oxide. J. Chem. Phys., 24, 95-97, 1956.
• LAKSHMI K„ RAO K.N., NIELSEN H.H. Molecular constants of nitrous oxide from measurements of v2 at 17 nm- J- Chem. Phys., 24, 811-813, 1950.
• LAANE J., OHLSEN J.R. Characterization of nitrogen oxides by vibrational spectroscopy. Prog. Inorg. Chem., 27, 465-513, 1980.
• GOLDEN B.M., YEUNG E.S. Analytical lines for long-path infrared absorption spectrometry of air pollutants. Anal. Chem, 47, 2132-2135,1975.
• LEFRS J.B, VAN DEN BERG P.J. Determination of nitrogen oxides and nitric acid vapour by infrared spectrometry. Anal. Chem, 52, 1424-1426,1980.
• LA VERY D.S. Environmental science, in Infrared and Raman Spectroscopy (Brame E.G., Jr. and Grasselli J.G., eds.). Part B, Marcel Dekker Inc.: New York and Basel, pp. 565-622, 1977.
• GRIFFITH D.W.T, FTIR, Bushfires, and atmospheric chemistry. 8th International Conference on Fourier Transform Infrared Spectroscopy (Heise H.M, Korte E.H, Siesler H.W, eds,), Proc. SPIE, 1575, 59-69,1992.
• ZAWADZKI J. Zastosowanie spektrometrii w podczerwieni do analizy spalin. I. Oznaczanie stężenia CO, C02 i S02. Ochrona Powietrza, 27, 142-145, 1992.
• ZAWADZKI J. Zastosowanie spektrometrii w podczerwie¬ni do analizy spalin. H. Badania nad oznaczaniem NO„. Ochrona Powietrza, 28, 7-9,1993.
• GUELACHVILI G, RAO K.N, Handbook of Infrared Standards. Academic Press Inc, 1986.
• AHO M, LINNA V, RANTANEN J, N2Q measurement technique using FT-IR spectroscopy. Poster at 1st Topic Orientated Technical Meeting of the International Flame Research Foundation: Amsterdam, 17-19 October, 1989.
• LINNA V. IR spectroscopy and acoustic pyrometry in combustion studies - report. Technical Research Center of Finland (VTT). Combustion and Thermal Engineering La¬boratory (PLT): Jyvaskyla, Finland, pp. 649-666, 1993.
• RYCZKOWSKI J. Application of IR spectroscopy for N20 determination. II. Determination of low nitrous contents using double beam spectrometer. Pol. J. Environm. Stud, submitted for publication.