Identification of gadoid species [Pisces, Gadidae] by PCR-RFLP analysis

• Autorzy: Aranishi F , Okimoto T , Izumi S
• Języki publikacji: EN

Abstrakty

EN

A rapid PCR-RFLP analysis was optimized to identify the presence of 3 closely related gadoid fish species: Alaska pollack Theragra chalcogramma, Pacific cod Gadus macrocephalus and Atlantic cod Gadus morhua in commercial seafood products. Gadoid universal primers were designed for PCR amplification of a 558-bp fragment encoding the mitochondrial cytochrome b gene. Without purification of the PCR products, double digestion with Eco32l and Eco1051 restriction enzymes generated reproducible species-specific restriction patterns visualizing 3 fragments (106 bp, 161 bp and 291 bp) in Alaska pollack and 2 fragments (106 bp and 452 bp) in Pacific cod, whereas no cleavage was observed in Atlantic cod. This PCR-RFLP analysis is simple, rapid and reliable, and therefore can be routinely applied to discover fraudulent substitution among 3 economically important gadoid species in commercial seafood products.

Słowa kluczowe

EN

cytochrome b; gadoid species; Pisces; polymerase chain reaction; species identification; Gadidae; food inspection; RFLP analysis; identification

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2005
• Tom: 46
• Numer: 1
• Opis fizyczny: p.69-73,fig.,ref.

Twórcy

autor

Aranishi F

• Department of Biological and Environmental Sciences, Miyazaki University, Miyazaki 889-2192, Japan

autor

Okimoto T

• Graduate School of Science and Technology, Nagasaki University, Nagasaki, Japan

autor

Izumi S

• Gunma Prefectural Fisheries Experiment Station, Maebashi, Japan

Bibliografia

• Albert B, Lewis D, Raff J, Roberts K, Watson JD, 1994. Molecular Biology of the Cell, Garland Publishing, New York.
• Aranishi F, Okimoto T, 2004. PCR-based detection of allergenic mackerel ingredients in seafood. J Genet 83: 193-195.
• Bossier P, 1999. Authentication of seafood products by DNA patterns. J Food Sci 64: 189-193.
• Cary SC, 1996. PCR-based method for single egg and embryo identification in marine organisms. BioTechniques 21: 998-1000.
• Chow S, Clarke ME, Walsh PJ, 1993. PCR-RFLP analysis on thirteen western Atlantic snappers (subfamily Lutjaninae): A simple method for species and stock identification. Fish Bull 30: 207-224.
• Davidson WS, 1998. PCR techniques. In: Ashurst PR, Dennis MJ, eds. Analytical methods of food authentication. Blackie Academic & Professional, London: 182-203.
• Esposti MD, De Vries S, Crimi M, Ghelli A, Patarnello T, Meyer A, 1993. Mitochondrial cytochrome b: evolution and structure of the protein. Biochim Biophys Acta 1143:243-271.
• Hastein T, Hill BJ, Berthe F, Lightner DV, 2001. Traceability of aquatic animals. Rev Sci Tech 20: 564-583.
• Hsieh Y-HP, Chen F, Nur M, 1997. Rapid identification of cooked red snapper using isoelectric focusing. J Food Sci 62: 15-19.
• Jerome M, Lemaire C, Bautista JM, Fleurence J, Etienne M, 2003. Molecular phylogeny and species identification of sardines. J Agric Food Chem 51 : 43-50.
• Lindstrom DP, 1999. Molecular species identification of newly hatched Hawaiian amphidromous gobioid larvae. Mar Biotech 1: 167-174.
• Lockley AK, Bardsley RG, 2000. DNA-based methods for food authentication. Trends Food Sci Technol 11: 67-77.
• Mackie IM, 1996. Authenticity of fish. In: Ashurt PR, Dennis MJ, eds. Food authentication. Blackie Academic & Professional, London: 140-170.
• Martinez I., Friis JT, 2004. Application of proteome analysis to seafood authentication. Proteomics 4: 347-354.
• Pope B, Kent HM, 1996. High efficiency 5 min transformation of Escherichia coli. Nucleic Acids Res 24: 536-537.
• Quinteiro J, Sotelo CG, Rehbein H, Pryde SE, Perez-Martin RI, Rey-Mendez M, Mackie IM, 1998. Use of mtDNA direct polymerase chain reaction (PCR) sequencing and PCR-restriction fragment length polymorphism methodologies in species identification of canned tuna. J Agric Food Chem 46: 1662-1669.
• Ram JL, Ram ML, Baidoun FF, 1996. Authentication of canned tuna and bonito by sequence and restriction site analysis of polymerase chain reaction products of mitochondrial DNA. J Agric Food Chem 44: 2460-2467.
• Rehbein H, Etienne M, Jerome M, Hattula T, Knudsen LB, Jessen F, et al. 1995. Influence of variation in technology on the reliability of the isoelectric focusing method of fish species identification. Food Chem 52: 193-197.
• Russell VJ, Hold GL, Pryde SE, Rehbein H, Quinteiro J, Rey-Mendez M, et al. 2000. Use of restriction fragment length polymorphism to distinguish between salmon species. J Agric Food Chem 48: 2184-2188.
• Wasko AP, Martins C, Oliveira C, Foresti F, 2003. Non-destructive genetic sampling in fish: An improved method for DNA extraction from fish fins and scales. Hereditas 138: 161-165.
• Yue GH, Orban L, 2001. Rapid isolation of DNA from fresh and preserved fish scales for polymerase chain reaction. Mar Biotech 3: 199-204.