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1997 | 19 | 4 |

Tytuł artykułu

Transgenes in plants: protection against viruses and insects

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Protective transgenes introduced into plants can be classified as directed against insects, fungi, bacteria or viruses. Mechanisms by which they protect plants in some cases are relatively simple and understood while in most cases they present only the field of rapidly progressing investigations. A brief review of the recent concepts of the resistance induced in plants against viruses by virus-derived transgenes is presented with emphasising the RNA mediated resistance. The RNA mediated resistance seems to operate in Nicotiana benthamiana plants transformed in our laboratory with cDNA of the PPV CP gene: both translatable and untranslatable versions of the cDNA made the transformed plants resistant against PPV. The resistant plants contained more than one copy of the transgene. To protect against insects plants were in our laboratory transformed with potato proteinase inhibitor II gene (PPI-II). The PPI-II gene expressed in model plants inhibited trypsin activity to an expected level.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

19

Numer

4

Opis fizyczny

p.561-569,fig.

Twórcy

  • A.Mickiewicz University, Miedzychodzka 5, 60-371 Poznan, Poland
autor

Bibliografia

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  • Bingham P.M. 1997. Cosuppression comes to the animals. Cell, 90: 385–387.
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  • Cogoni C., Irelan J.T., Schumacher M., Schmidhauser T.J., Selker E.U., Macino G. 1996. Transgene silencing of the al-1 gene in vegetative cells of Neurospora is mediated by a cytoplasmic effector and does not depend on DNA-DNA interactions or DNA methylation. EMBO J., 15: 3153–3163.
  • Dougherty W.G., Lindbo J.A., Smith H.A., Parks T.D., Swaney S., Proebsting W.M., 1994. RNA-mediated virus resistance in transgenic plants: exploitation of a cellular pathway possibly involved in RNA degradation. Mol. Plant Microbe. Interact., 7: 544–552.
  • English J.J., Mueller E., Baulcombe D.C. 1996. Suppression of virus accumulation in transgenic plants exhibiting silencing of nuclear genes. Plant Cell, 8: 179–188.
  • Hofte H., Whiteley H.R. 1989. Insecticidal crystal proteins of Bacillus thuringiensis. Microbiol. Rev., 53: 242–255.
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  • Li J. 1996. Insecticidal d-endotoxins from Bacillus thuringensis. In: Protein Toxin Structure, ed. by M.W. Parker. R.G. Landes Company: 50–77.
  • Lindbo J.A., Dougherty W.G. 1993. Pathogen-derived resistance to a potyvirus: immune and resistant phenotypes in transgenic tobacco expressing altered forms of a potyvirus coat protein nucleotide sequence. Mol. Plant Microbe. Interact., 5:144–153.
  • McGurl B., Pearce G., Orozco-Cardenas M., Ryan C.A. 1992. Structure, expression, and antisense inhibition of the systemin precursor gene. Science, 255:1570–1573.
  • McGurl B., Orozco-Cardenas M., Pierce G., Ryan C.A. 1994. Overexpression of the prosystemin gene in transgenic tomato plants generates a systemic signal that constitutively induces proteinase inhibitor synthesis. Proc. Natl. Acad. Sci. USA 91: 9800–9802.
  • Metzlaff M., O’Dell M., Cluster P.D., Flavell R.B. 1997. RNA-mediated RNA degradation and chalcone synthase A silencing in petunia. Cell, 88: 845–854.
  • Misztal L.H., Musiał W.G., Augustyniak J. 1996. Insecticidal toxins of Bacillus thuringensis. Post. Mikrobiol., 35:193–211.
  • Pace N.R., Smith D. 1990. Ribonuclease P: function and variation. J. Biol. Chem. 265: 3587–3590.
  • Pal-Bhadra, M., Bhadra U., Birchler J.A. 1997. Cosuppression in Drosophila: Gene silencing of alcohol dehydrogenase by white-Adh transgenes is polycomb dependent. Cell, 90: 479–490.
  • Perlak F.J., Fuchs R.L., Dean D.A., McPherson S.L., Fischhoff D.A. 1991. Modification of coding sequences enhances plant expression of insect control protein genes. Proc.Natl.Acad.Sci. USA 88: 3324–3328.
  • Powell Abel P., Nelson R.S., De B., Hoffmann N., Rogers S.G., Fraley R.T., Beachy R.N. 1986. Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene. Science, 232: 738–743.
  • Sanchez-Serrano J.J., Keil M., O’Connor A., Schell J., Willmitzer L. 1987. Wound-induced expression of a potato proteinase inhibitor II gene in transgenic tobacco plants. The EMBO J., 6: 303–306.
  • Shade R.E., Schroeder H.E., Pueyo J.J., Tabe L.M., Murdock H.E., Higgins T.J.V., Chrispeeles M.J. 1994. Transgenic pea seeds expressing the b-amylase inhibitor of the common bean are resistant to Burchid beetles. Biotechnology, 12: 793–796.
  • Shukle R.H., Murdock L.L. 1983. Lipoxygense, trypsin inhibitor, and lectin from soybeans: effects on larval growth of Manduca sexta (Lepidoptera: Sphingidae). Environ. Entomol., 12: 787–791.
  • Sijen T., Wellink J., Hiriart J.B., van Kammen A. 1996. RNA-mediated virus resistance: role of repeated transgenes and delineation of targeted regions. Plant Cell, 8: 2277–2294.
  • Silva-Rosales L., Lindbo J.A., Dougherty, W.G. 1994. Analysis of transgenic tobacco plants expressing a truncated form of a potyvirus coat protein nucleotide sequence. Plant Mol. Biol., 24: 929–939.
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  • Smith H.A., Swaney S.L., Parks T.D., Wernsman E.A., Dougherty W.G. 1994. Transgenic plant virus resistance mediated by untranslatable sense RNAs: expression, regulation, and fate of nonessential RNAs. Plant Cell, 6; 1441–1453.
  • Smith H.A., Powers H., Swaney S.L., Brown C., Dougherty W.G. 1995. Transgenic potato virus Y resistance in potato: evidence for an RNA-mediated cellular response. Phytopathology, 85: 864–870.
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  • Thierry D., Vaucheret H. 1996. Sequence homology requirements for transcriptional silencing of 35S transgenes and post-transcriptional silencing of nitrite reductase (trans)genes by the tobacco 271 locus. Plant Mol. Biol., 32: 1075–1083.
  • Wypijewski, K., Korcz-Szatkowska, H., Cajza, M., Augustyniak, J. 1996. Fertile transgenic Nicotiana benthamiana plants carrying a capsid protein cDNA of PPV. J. Appl. Genet (former Genet. Pol.) 37A: 136–139.
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Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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