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2001 | 47 | 4 |

Tytuł artykułu

Analiza przebiegu doswiadczalnej pneumocystozy u szczurow za pomoca wybranych metod serologicznych

Treść / Zawartość

Warianty tytułu

Języki publikacji

PL

Abstrakty

EN
Since serological methods used in the diagnosis of pneumocystosis may be helpful to a various extent, depending on the stage of a diagnosed infection, it was decided to evaluate the usefulness of some the methods in the course of infection. Wistar rats were used in the experiments. The animals were administered hydrocortisone infections for 12 weeks to induce immunosuppression and activate naturally occurring asymptomatic infections with Pneumocystis carinii. Blood samples and specimes of lung tissues were collected, then they were examined for the presence of specific IgG and IgM antibodies, circulating antigen of Pneumocystsis carinii and circulating immune complexes using immunoenzymatic assays. The results of the above experiments indicated, that in an early stage of infection, the examinations of serum samples for circulating immune complexes were helpful, particularly for these with IgM antibodies.

Wydawca

-

Rocznik

Tom

47

Numer

4

Opis fizyczny

s.717-721,tab.,bibliogr.

Twórcy

autor
  • Panstwowy Zaklad Higieny, ul.Chocimska 24, 00-791 Warszawa
autor
autor

Bibliografia

  • ALIOUAT E. M., MARTINEZ A., JIMENEZ E., DEI-CAS E., MUULET C., MELCOURT P. GARGALLO-VIOLA D. 1997. Development of pneumocystosis animal models: corticosteroid-treated Wistar rat; SCID mouse and nude rat. J. Euk. Microbiol. 44: 41-42.
  • BROOKS R. G., SHARMA S. D., REMINGTON J. S. 1985. Detection of Toxoplasma gondii antigens by a dot-immunobinding technique. J. Clin. Microbiol. 21: 113-116.
  • FRENKEL J. K., GOOD J. T., SCHULTZ J. 1965. Pathogenesis and chemotherapy of Pneumocystis carinii infection of rats. In: Progress in Protozoology, Int. Congr. Ser.. No 91 p. 129, Excerpta Med. Found. Amsterdam.
  • GRAVES C. 1989. Immunological studies of Pneumocystis carinii. J. Protozoal. 36: 60-69.
  • KOVACS J. A., HALPERN J. L., LUNDGREN B., SWAN J. C., PARRILLO J. E., MASUR H. 1989. Monoclonal antibodies to Pneumocystis carinii: Identification of specific antigens and characterization of antigenic differences between rat and human isolates. J. Infect. Dis. 159: 60-69.
  • VAN KNAPEN F., PANGGABEAN O., VAN LEUSDEN J. 1985. Demonstration of Toxoplasma antigen containing complexes in active toxoplasmosis. J. Clin. Microbiol. 22: 645-650.
  • PIFER L. L., PIFER D. D., WOODS D. R., JOYNER R. E., EDWARDS C. C. 1986. Preliminary studies on the development of a vaccine for Pneumocystis carinii. I. Immunological and biochcmical characterization. Vaccine 4: 257-265.
  • SOBOLEWSKA A., DZBEŃSKI T. H. 1997. Epidemiologia i diagnostyka laboratoryjna inwazji powodowanych przez Pneumocystis carinii. Przeg. Epid. 51: 1-2.

Typ dokumentu

Bibliografia

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