Molecular detection and comparison of Gaeumannomyces graminis var. tritici isolates originating from wheat and rye

• Autorzy: Irzykowska L

Warianty tytułu

PL

Molekularna identyfikacja oraz porownanie izolatow Gaeumannomyces graminis var. tritici pochodzacych z pszenicy i zyta

• Języki publikacji: EN

Abstrakty

EN

Gaeumannomyces graminis is an etiologic agent of take-all, economically important disease of cereals worldwide. A polymerase chain reaction with variety-specific primers was successfully used for detection of G. graminis var. tritici in plant tissue. Obtained results showed that this diagnostic method is a very sensitive and useful tool for detection of the pathogen even before disease symptoms arise. DNA polymorphism revealed by RAPD-PCR with three arbitrary primers was suitable for assessing genetic variation among Ggt isolates originating from wheat and rye.

PL

Gaeumannomyces graminis jest czynnikiem etiologicznym zgorzeli podstawy źdźbła i korzeni, ekonomicznie ważnej choroby zbóż o zasięgu światowym. W badaniach zastosowano łańcuchową reakcję polimerazy z odmianowo-specyficznymi starterami do wykrywania Gaeumannomyces graminis var. tritici (Ggt) zarówno w czystych kulturach jak i tkance roślinnej. Na podstawie uzyskanych wyników stwierdzono, że zastosowana metoda diagnostyczna jest bardzo czułym i użytecznym narzędziem do wykrywania patogena jeszcze przed wystąpieniem objawów choroby na korzeniach. Polimorfizm DNA ujawniony na drodze analizy RAPD-PCR z trzema losowymi starterami był wystarczający do określenia zróżnicowania genetycznego izolatów Ggt pochodzących z pszenicy i żyta.

Słowa kluczowe

EN

molecular detection; comparison; Gaeumannomyces graminis var.tritici; isolate; wheat; rye; random amplified polymorphic DNA analysis; molecular analysis

• Wydawca: -
• Czasopismo: Journal of Plant Protection Research
• Rocznik: 2007
• Tom: 47
• Numer: 3
• Opis fizyczny: p.299-308,fig.,ref.

Twórcy

autor

Irzykowska L

• The August Cieszkowski Agricultural University, Dabrowskiego 159, 60-954 Poznan, Poland

Bibliografia

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