Atypical bacterial gill disease in rainbow trout [Oncorhynchus mykiss]: influence on non specific humoral defence mechanisms

• Autorzy: Siwicki A K , Pozet F , Morand M , Kazun K , Glabski E , Trapkowska S
• Języki publikacji: EN

Abstrakty

EN

Rainbow trout (10-20 g body weight) from a local fish farm were examined after low levels of mortality at a temperature of 10°C were observed. Dystrophic changes were observed only in the gills. Based on its unusual clinical presentation, this condition was termed atypical bacterial gill disease (ABGD). Only Flavobacterium psychrophilum was isolated from the gills. On the day the first symptoms of the disease appeared and on the third, seventh, and tenth days afterwards blood from 20 afflicted fish (diseased) and 20 unafflicted fish was drawn to examine the nonspecific humoral defense mechanism. In the present immunological studies a strong immunosuppression effect was observed in fish with symptoms of atypical bacterial gill disease. The results showed that serum lysozyme activity and Ig levels were statistically (P < 0.05) lower in fish with disease symptoms in comparison with control, disease-frie fish. The activity of serum ceruloplasmine was very high in fish with disease symptoms in comparison with the control fish. The results indicated that the suppression occurred as atypical bacterial gill disease was developing. All immunological parameters were statistically significantly lower after three, seven, and ten days of disease symptoms in comparison with the disease-free, control fish. This preliminary study indicated that ABGD with Flavobacterium psychrophilum has a strong suppression effect on the nonspecific humoral defense mechanisms.

PL

W ostatnich latach obserwuje się znaczny wzrost zachorowań narybku pstraga tęczowego na atypową bakteryjną chorobę skrzeli, która powoduje znaczące straty w hodowli. Zmiany dotyczą jedynie skrzeli z których izoluje się najczęściej Flavobacterium psychrophilum, Flavobacterium branchiophilum. W prezentowanych badaniach określano wpływ atypowej bakteryjnej choroby skrzeli wywołanej przez Flavobacterium psychrophilum na wybrane nieswoiste humoralne mechanizmy obronne u narybku pstrąga tęczowego. Uzyskane wyniki badań jednoznacznie wykazały, że w czasie rozwoju choroby stwierdzono statystycznie istotne (P < 0,05) obniżenie aktywności lizozymu oraz poziomu Ig w surowicy badanych ryb, w porównaniu z grupą kontrolną, u której nie stwierdzono zmian chorobowych oraz nie izolowano Flavobacterium psychrophilum. W tym okresie stwierdzono statystycznie istotny wzrost aktywności ceruloplazminy, przy braku zmian w poziomie białka całkowitego. Wstępne badania sugerują, że Flavobacterium psychrophilum może indukować supresję nieswoistych mechanizmów obronnych, co pozwala na przełamanie bariery odpornościowej i kliniczny rozwój choroby.

Słowa kluczowe

EN

Oncorhynchus mykiss; atypical bacterial gill disease; gill disease; Flavobacterium psychrophilum; rainbow trout; disease control; non-specific humoral immunity; aquaculture

• Wydawca: -
• Czasopismo: Archives of Polish Fisheries
• Rocznik: 2004
• Tom: 12
• Numer: 1
• Opis fizyczny: p.5-12,fig.,ref.

Twórcy

autor

Siwicki A K

• The Stanislaw Sakowicz Inland Fisheries Institute in Olsztyn, Olsztyn, Poland
• Department of Microbiology and Clinical Immunology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Poland

autor

Pozet F

• Laboratoire Depatemental d'Analyses, Conseil General du Jura, France

autor

Morand M

• Laboratoire Depatemental d'Analyses, Conseil General du Jura, France

autor

Kazun K

• The Stanislaw Sakowicz Inland Fisheries Institute in Olsztyn, Olsztyn, Poland

autor

Glabski E

• The Stanislaw Sakowicz Inland Fisheries Institute in Olsztyn, Olsztyn, Poland

autor

Trapkowska S

• Department of Microbiology and Clinical Immunology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Poland

Bibliografia

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