Lymphoproliferation assay in cattle naturally infected with bovine leukemia virus [BLV] and bovine immunodeficiency virus [BIV]

• Autorzy: Stec J , Bicka L. , Kozaczynska B. , Kuzmak J.
• Języki publikacji: EN

Abstrakty

EN

Lymphocyte proliferation rate was determined by ³H-thymidine incorporation assay after stimulation of peripheral blood mononuclear cells (PBMC) from BLV and BIV-infected cattle with mitogens: concanavalin A, phytohaemagglutitin, and pokeweed mitogen. BLV infection were associated with significant decrease in lymphocyte proliferation, as compared to uninfected animals. Co-infection with BIV strongly increased antimitotic influence of BLV, mainly in PL-animals. These results demonstrated that a function impairement of lymphocytes can be observed in the course of BLV and/or BLV/BIV infection.

Słowa kluczowe

EN

retrovirus; proliferation; lymphocyte transformation; cattle; Retroviridae; bovine leukemia virus; bovine immunodeficiency virus

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2001
• Tom: 45
• Numer: 2
• Opis fizyczny: p.117-123,fig.

Twórcy

autor

Stec J

• National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Bicka L.

autor

Kozaczynska B.

autor

Kuzmak J.

Bibliografia

• 1. Cockerell G.L., Parodi A.L., Levy D.: Immunocompetance of sheep experimentally infected with bovine leukemia virus. Vet. Immunol. Immunopathol., 1986, 13, 189-196.
• 2. Flaming K.P., Frank D.E., Carpenter S., Roth J.A.: Longitudinal studies of immune function in cattle experimentally infected with bovine immunodeficiency-like virus and/or bovione leukemia virus. Vet. Immunol. Immunopathol., 1997, 56, 27-35.
• 3. Gatei M., Brandon R., Naif H., McLennan M., Daniel R., Lavin M.: Changes in B and T cell subsets in bovine leukemia virus-infected cattle. Vet. Immunol. Immunopathopl., 1989, 23, 139-147.
• 4. Ghysdael J., Bruck C., Kettmann R., Burny A.: Bovine leukemia virus. Current TopicinMicrobiology and Immunology. 1984, Vol. 112, Springer-Verlag Berlin Heidelberg, pp.1-19.
• 5. Haraguchi S., Good R., Day N.: lmmunosupressive retroviral peptides: cAMP and cytokine patterns. Immunol. Today, 1995, 16, 595-603.
• 6. Heaton P., Johnstone P., Brownlie J.: Investigation of cellular tropism of bovine immunodeficiency-like virus. Res. Vet. Sci., 1998, 65, 33-40.
• 7. Hidalgo G., Bonilla A.: Lymphoproliferation assays in cattle naturally infected with bovine leukemia virus (BLV) and bovine immunodeficiency virus (BIV). J. Vet. Med. B., 1996, 43, 325-332.
• 8. Hirai, N., Kabeya, H., Ohashi, K., Sugimoto, C., Onuma M.: Immunomodulative effects of bovine immunodeficiency-like virus (BIV)-infecton and mixed infection of BIV and bovine leukemia virus in sheep. J.J. Vet. Res., 1996, 44, 153-161.
• 9. Keles I., Sharma A., Woldehiwet Z., Murray R.: The effect of bovine respiratory syncytial virus on normal ovine lymphocyte responses to mitogens or antigens in vitro. Comp. Immunol. Microbiol. Infec. Dis., 1999, 22, 1 -13.
• 10. Kettmann R., Dechamps J., Cleuter Y., Ghysdael J., Mammerickx M.: Distribution of bovine leukemia virus proviral DNA sequences in tissue of animals with enzootic bovine leukosis. Leuk. Res., 1978, 2, 23-32.
• 11. Maes S., Kabeya H., Yohihara S., Ohasi K., Matsuki S., Mikami Y., Sugimoto C., Onuma M.: Seroprevalence and field isolation of bovine immunodeficiency virus. J. Vet. Med. Sci., 1998, 11, 1195-1202.
• 12. Martin S.J., O'Neil T.P., Bilello J.A., Eiseman J.L.: Lymphocyte transformation abnormalities in bovine immunodeficiency-like virus infected calves. lmmunol. Letters, 1991, 27, 81-85.
• 13. Onuma M., Wada M., Yasutomi Y., Yamamoto M., Okada H., Kawakami Y.: Suppression of immunological responses in rabbits experimentally infected with bovine leukemia virus. Vet. Microbiol., 1990, 25, 131-138.
• 14. Schwartz I., Bensaid A., Polack B., Perrin B., Berthelemy M., Levy D.: In vivo leukocyte tropism of bovine leukemia virus in sheep and cattle. J. Virol. 1994, 68, 4589-4596.
• 15. Snider T.G., Luther D., Jenny B., Hoyot P., Batteles J., Ennis W., Balady J., Blas U., Lemarschand T., Gonda M.: Encephalitis, lymphoid tissue depletion and secondary diseases associated with bovine immunodeficiency virus in a dairy herd. Comp. Immun. Microbiol. Infect. Dis., 1996, 19, 117-131.
• 16. Stec J., Grundboeck J., Szczotka M., Reichert M., Rulka J., Kozaczynska B.: Effect of mitogens on the in vitro stimulation of lymphocytes from normal and BLV-infected cattle. Bull. Vet. Inst. Pulawy, 1993, 33, 29-35.
• 17. Stec J., Kuźmak J: Effect of infection with bovine immunodeficiency virus and/or bovine leukemia virus on lymphocyte blastogenesis to mitogens in sheep. Bull. Vet. Inst. Pulawy, 2001, 45, 21-27.
• 18. Takashima I., Olson C.: Effect of mitogens and anti-bovine leukosis virus serum on DNA synthesis on lymphocytes from cattle. Europ. J. Cancer, 1980, 16, 639-645.
• 19. Trainin Z., Brenner J., Meirom R., Ungar-Waron H.: Detrimental effect of bovine leukemia virus (BLV) on the immunological state of cattle. Vet. Immunol. Immunopathol., 1996, 54, 293-302.
• 20. Whetstone C., Suarez D., Miller J., Pesch B., Harp J.: Bovine lentivirus induces early transient B-cells proliferation in experimentally inoculated cattle and appears to be pantropic. J. Virol., 1997, 71, 640-640.