Developing of DNA-marker to the Fusarium oxysporum f.sp. lycopersici resistance genes of tomato

• Autorzy: Amini J

Warianty tytułu

PL

Opracowanie markerow DNA dla genow odpornosci pomidora na Fusarium oxysporum f.sp. lycopersici

• Języki publikacji: EN

Abstrakty

EN

Fusarium wilt caused by Fusarium oxysporum f. sp. lycopersici is a destructive disease of tomato crops worldwide. The use of resistant varieties is the best strategy for disease control. In the present study we analyze eight tomato lines and hybrids for Fusarium wilt disease resistance by polymerase chain reaction. Total genomic DNA was extracted from young leaves of three-week-old plants of tomato. Results of PCR of eight tomato lines and hybrids indicated that there are one dominant heterozygote, two recessive homozygotes and five dominant homozygotes. Also, results of polymerase chain reaction showed that it needs less time and is cheaper. Also by using this method, it is possible to determine genotype of plant (homozygote or heterozygote) without presence of the pathogen. Therefore, PCR technique was used in the identification gene I2 conferring resistance to F. oxysporum f. sp. lycopersici.

PL

Więdnięcie pomidora wywoływane przez Fusarium oxysporum f. sp. lycopersici jest destruktywną chorobą w skali światowej. Najlepszą straegią zwalczania choroby jest wykorzystywanie odpornych odmian. W pracy przeanalizowano osiem linii i mieszańców pomidora na odporność przeciwko więdnięciu fuzaryjnemu, wykorzystując łańcuchową reakcję polimerazy (PCR).

Słowa kluczowe

EN

polymerase chain reaction; disease control; plant disease; wilt disease; DNA marker; resistance gene; tomato; plant protection; Fusarium oxysporum f.sp.lycopersici

• Wydawca: -
• Czasopismo: Journal of Plant Protection Research
• Rocznik: 2009
• Tom: 49
• Numer: 2
• Opis fizyczny: p.175-178,fig.,ref.

Twórcy

autor

Amini J

• University of Kurdistan, P.O.Box 416, Sanandaj, Iran

Bibliografia

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