Wytwarzanie enterotoksyny przez szczepy Bacteroides fragilis - wplyw klindamycyny

• Autorzy: Rokosz A. , Rouyan G.S. , Meisel-Mikolajczyk F.
• Języki publikacji: PL

Abstrakty

EN

Four B. fragilis strains were examined: one nonenterotoxigenic (NTBF) and three producing enterotoxin (ETBF). The growth of cultures was determined and enterotoxin, which is released to the culture medium during growth of strains, was detected. BHI broth and BHI broth with addition of subinhibitory doses (sub-MIC) of clindamycin were applied. Bacterial cultures were incubated at 37°C for 48 hours. After 4, 8, 16, 24, 48 hours of cultivation, samples of bacterial cultures were collected and the optical density was measured. Then the samples were centrifuged, supernatants were filtered through 0,45 µm filters and concentrated three times with 5000 D uItrafiIters. Prepared samples were kept frozen at -70°C until used. The titre of enterotoxin in samples was determined on human colon adenocarcinoma cell line HT 29/C1. Neutralization assay was performed with culture filtrates, which were enterotoxin-positive and with rabbit anti-enterotoxin serum. The results of the experiments indicate that enterotoxin is detected after 16 hours of incubation of ETHF strains. Clindamycin at subinhibitory concentrations (sub-MIC) inhibits the growth of B. fragilis cultures. The antibiotic causes also delay and decrease in enteriotoxin production by ETBF strains.

Słowa kluczowe

PL

hodowla mikroorganizmow; klindamycyna; szczepy bakteryjne; Bacteroides fragilis; enterotoksyny; wytwarzanie

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 1997
• Tom: 49
• Numer: 3-4
• Opis fizyczny: s.153-159,tab.,bibliogr.

Twórcy

autor

Rokosz A.

• Akademia Medyczna, 02-400 Warszawa, ul.T.Chalubinskiego 5

autor

Rouyan G.S.

autor

Meisel-Mikolajczyk F.

Bibliografia

• 1. Donelli G., Fabbri A., Fiorentini C.: Infect. Immun., 1996, 41, 113.
• 2. Dowell V.R., Hawkins T.M.: Laboratory methods in anaerobic bacteriology, CDC Lab. Manual, D.E.H.W. Atlanta, 1974.
• 3. Kato N., Karuniawati A., Jotwan R., Kato H., Ueno K.: 94 th ASM General Meeting, 1994, Session 25, B-29.
• 4. Koshy S.S., Montrose M.H., van Tassel R.L., Sears C.L.: 94 th ASM General Meeting, 1994, Session 25, B-26.
• 5. Meisel-Mikołajczyk F.: Post. Mikrobiol., 1995, 34, 197.
• 6. Meisel-Mikołajczyk F.,Sebald M., Torbicka E., Rafałowska K., Zielińska U.: Acta Microbiol. Polon., 1994, 43, 389.
• 7. Moncrief J.S., Obiso R.J.Jr., Barroso L.A., Kling J.J., Wright R.L., van Tassel R.L., Lyerly D.M., Wilkins T.D.: Infect. Immun., 1995, 63, 175.
• 8. Myers L.L., Firehammer B.D., S hoop D.S., Border M.M.: Infect. Immun., 1984, 44, 241.
• 9. Myers L.L., Shoop D.S.: Am. J. Vet. Res., 1987, 48, 774.
• 10. Myers L.L., Shoop D.S., Byars T.D.: Am. J. Vet. Res., 1987,48, 1565.
• 11. Myers L.L., Shoop D.S., Firehammer B.D., Border M.M.: J. Infect. Dis., 1985, 152, 1344.
• 12. Myers L.L., Shoop D.S., Stackhouse L.L., Newman S.S., Flaherty R.J., Letson G.W., Sack R.B.: J. Clin. Microbiol., 1987, 25, 2330.
• 13. Obiso R.J.Jr., Azghani A. O., Wilkins T.D.: Infect. Immun., 1997, 65, 1431.
• 14. Pantosti A., Cerquetti M., Colangeli R., D’Ambrosia F.: J. Med. Microbiol., 1994, 41,191.
• 15. Pantosti A., Tzianabos A.O., Reinap B.G., Onderdonk A.B., Kasper D.L.: J. Clin. Microbiol., 1993, 31, 1850.
• 16. Rokosz A., Meisel- Mikołajczyk F.: Med. Dośw. Mikrobiol., 1995, 47, 183.
• 17. Stevens D.L., Bryant A.E., Hackett S.P.: Clin. Infect. Dis., 1995, 20 (Suppl. 2), S154.
• 18. van Tassel R.L., Lyerly D.M., Wilkins T.D.: Infect. Immun., 1992, 60, 1343.
• 19. Weikel C.S., Grieco F.D., Myers L.L.: 27 th Joint Conf. Cholera Relat. Dis. Abstr., 1991, 25.
• 20. Weikel C.S., Grieco F.D., Reuben J., Myers L.L., Sack R.B.: Infect. Immun., 1992, 60, 321.