Ornithine decarboxylase in large bowel mucosa: regulation by gastrin, secretin and EGF

• Autorzy: Johnson L R , Wang P. , Haddox K.
• Języki publikacji: EN

Abstrakty

EN

Rats were fasted 48 h and then injected once with either saline, pentagastrin, EGF, secretin or combinations of secretin and pentagastrin or EGF. Another group of rats was fasted and refed. Animals were killed 4 h later and ODC assayed in mucosa of the cecum, proximal colon, and distal colon. EGF significantly increased ODC activity in all 3 tissues. Secretin had no effect by itself on ODC or ODC stimulated by EGF. Pentagastrin significantly increased ODC of the cecum, and secretin completely inhibited the effect of pentagastrin. Refeeding fasted rats significantly induced activity in all three tissues. Immunocytochemistry using a highly specific polyclonal ODC antibody showed that ODC was confined to the crypt cells of the proximal colon. Antibody dilution techniques demonstrated that gastrin, EGF and refeeding increased the level of enzyme in these cells. Refeeding in addition caused the appearance of enzyme in surface epithelial cells. These results showed that colonic mucosal ODC is present in proliferative cells and is regulated by the same peptides known to regulate growth in this tissue. Colonic mucosal ODC also responds the same way as it does in the oxyntic gland and small bowel mucosa.

Słowa kluczowe

EN

polyamine; secretin; ornithine decarboxylase; large bowel mucosa; colonic mucosa; rat; gastrin; hormone

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 1992
• Tom: 43
• Numer: 1
• Opis fizyczny: p.33-41,fig.

Twórcy

autor

Johnson L R

• University of Tennessee Nash Research Building 894 Union Avenue Memphis, Tennessee 38163, USA

autor

Wang P.

autor

Haddox K.

Bibliografia

• 1. Johnson LR. Regulation of gastrointestinal mucosal growth. Physiol Rev 1988; 68: 456-502.
• 2. Johnson LR, Guthrie PD. Stimulation of rat oxyntic gland mucosal growth by epidermal growth by epidermal growth factor. Am J Physiol 1980; 238: G45-G49.
• 3. Seidel ER, Tabata K, Dembinski AB, Johnson LR. Attenuation of the trophic response to gastrin after inhibition of ornithine decarboxylase. Am J Physiol 1985; 149: G16-20.
• 4. Johnson LR, Tseng C-C, Tipnis UR, Haddox MK. Gastric mucosal ornithine decarboxylase: localization and stimulation by gastrin. Am J Physiol 1988; 255: G304-G312.
• 5. Johnson LR, Tseng C-C, Wang P, Tipinis UR, Haddox MK. Mucosal ornithine decarboxylase in the small intestine: localization and stimulation. Am J Physiol 1989; 256: G24-G30.
• 6. Feldman EJ, Aures D, Grossman MI. Epidermal growth factor stimulates ornithine decarboxylase activity in the digestive tract of mouse. Proc Soc Exp Biol Med 1978; 159: 400-402.
• 7. Fitzpatrick LR, Wang P, Johnson LR. Effect of epidermal growth factor on polyamine-synthesizing enzymes in rat enterocytes. Am J Physiol 1987; 252: G209-G214.
• 8. Tabata K, Johnson LR. Ornithine decarboxylase and mucosal growth in response to feeding. Am J Physiol 1986; 251: G270-G274.
• 9. Pegg AE, McCann PP. Polyamine metabolism and function. Am J Physiol 1986; 143: C212-C221.
• 10. Russell DH, Snyder SH. Amine synthesis in rapidly growing tissues: ornithine decarboxylase activity in regenerating rat liver, chick embryo and various tumors. Proc Natl Acad Sci USA 1968; 60: 1420-1427.
• 11. Bradford MA. A rapid and sensitive method for the quantitation of microgram amounts of protein using the principle of protein-dye binding. Anal Biochem 1976; 72: 248-254.
• 12. Hsu SM, Raine L, Fanger H. Use of avidin-biotin peroxidase complex (ABC) in immunoperoxidase techniques: A comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem 1981; 29: 577-580.
• 13. Quian Z-U, Tsai Y-H, Steinberger A, Lu M, Greenfield ARL, Hadddx MK. Localization of ornithine decarboxylase in rat testicular cells and epididymal spermatozoa. Biol Reprod 1985; 33: 1189-1195.
• 14. Greenfield ARL, Taffet SM, Haddox MK. Immunocytochemical localization of ornithine decarboxylase in cultured murine cells. Cell Tissue Res 1986; 243: 33-40.
• 15. Takeuchi K, Speir GR, Johnson LR. Mucosal gastrin receptor. IV. Binding specificity. Am J Physiol 1980; 239: G395-G399.
• 16. Kumagai J, Johnson LR. Characteristics of putrescine uptake in isolated rat enterocytes. Am J Physiol 1988; 254: G81-G86.
• 17. Seidel ER, Haddox MK, Johnson LR. Illeal mucosal growth during infusion of ethylamine or putrescine. Am J Physiol 1985; 149: G234-G438.
• 18. Osborne DL, Seidel ER. Microflora-derived polyamines modulate obstruction-induced colonic mucosal hypertrophy. Am J Physiol 1989; 256: G1049-G1057.