Influence of ischemic preconditioning on blood coagulation, fibrinolytic activity and pancreatic repair in the course of caerulein-induced acute pancreatitis in rats

• Autorzy: Warzecha Z. , Dembinski A. , Ceranowicz P. , Dembinski M. , Cieszkowski J. , Kusnierz-Cabala B. , Naskalski J.W. , Jaworek J. , Konturek S.J. , Pawlik W.W. , Tomaszewska R.
• Języki publikacji: EN

Abstrakty

EN

Previous studies have shown that ischemic preconditioning protects several organs, including the pancreas, from ischemia/reperfusion-induced injury. The aim of the investigation was to determine whether ischemic preconditioning affects the course edematous pancreatitis. Methods: In rats, ischemic preconditioning was performed by short-term clamping the celiac artery. Acute pancreatitis was induced by caerulein. The severity of acute pancreatitis was evaluated between the first and tenth day of inflammation. Results: Ischemic preconditioning applied alone caused a mild pancreatic damage. Combination of ischemic preconditioning with caerulein attenuated the severity of pancreatitis in histological examination and reduced the pancreatitis-evoked increase in plasma lipase and pro-inflammatory interleukin-1ß. This effect was associated with an increase in plasma level of anti-inflammatory interleukin-10 and partial reversion of the pancreatitis-evoked drop in pancreatic DNA synthesis and pancreatic blood flow. In secretory studies, ischemic preconditioning in combination with induction of acute pancreatitis attenuated the pancreatitis-evoked decrease in secretory reactivity of isolated pancreatic acini to stimulation by caerulein. In the initial period of acute pancreatitis, ischemic preconditioning alone and in combination with caerulein-induced acute pancreatitis prolonged the activated partial thromboplastin time (APTT), increased plasma level of D-dimer and shortened the euglobulin clot lysis time. The protective effect of ischemic preconditioning was observed during entire time of experiment and led to acceleration of pancreatic regeneration. Conclusions: Ischemic preconditioning reduces the severity of caerulein-induced pancreatitis and accelerates pancreatic repair; and this effect is related to the activation of fibrinolysis and reduction of inflammatory process.

Słowa kluczowe

EN

pancreatic blood flow; interleukin 1beta; fibrinolytic activity; blood; caerulein-induced acute pancreatitis; rat; coagulation; ischaemic preconditioning

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2007
• Tom: 58
• Numer: 2
• Opis fizyczny: p.303-319,fig.,ref.

Twórcy

autor

Warzecha Z.

• Jagiellonian University Medical College, Grzegorzecka 16, 31-531 Krakow, Poland

autor

Dembinski A.

autor

Ceranowicz P.

autor

Dembinski M.

autor

Cieszkowski J.

autor

Kusnierz-Cabala B.

autor

Naskalski J.W.

autor

Jaworek J.

autor

Konturek S.J.

autor

Pawlik W.W.

autor

Tomaszewska R.