Organogenesis and somatic embryogenesis in cultures of Cyclamen persicum Mill. F1 Medium

• Autorzy: Bach A. , Malik M. , Zolneczko B.
• Języki publikacji: EN

Abstrakty

EN

Different explants (root and hypocotyl slices or leaf parts) isolated from aseptic seedlings of Cyclamen persicum Mill. F1 ‘Medium’ were exposed to various concentrations of auxins and cytokinins. On the media with a higher concentration of cytokinin (2iP at 25 µM) than auxin (NAA at 2.5 µM) all the explants produced low amounts of callus or else directly generated adventitious roots as well as shoots. Leaves proved to be the best explants for direct organogenesis. Adventitious shoots were produced mostly on leaf margins. Direct formation of adventitious roots was observed on the explants isolated from the central parts of seedling leaves. At the lowest concentration of 2,4-D, direct development of adventitious roots occurred regardless of the kind of explant. The medium with 50 µM 2,4-D and 4 µM 2iP was most effective in inducing caulogenesis. Somatic embryos developed on the medium with 0.5 µM 2,4-D and 5 µM BA. They were characterized by well-developed hypocotyls and roots, and inconspicuously formed cotyledons.

Słowa kluczowe

EN

callus culture; adventitious root; auxin; growth regulator; somatic embryogenesis; Cyclamen persicum; cytokinin; embryogenesis; organogenesis

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 1998
• Tom: 40
• Opis fizyczny: p.47-51,fig.

Twórcy

autor

Bach A.

• Agricultural University, Al.29 Listopada 54, 31-425 Krakow, Poland

autor

Malik M.

autor

Zolneczko B.

Bibliografia

• Bouman H., and A. M. Kuijpers. 1996. Quality of embryogenic callus of cyclamen induced by initial culture on high 2,4-D. Plant regeneration via suspension culture systems. Abstracts of the Third Meeting of the Working Group Cost 822, 5-8 September 1996, 13. Sanremo.
• Dillen W., I. Dijkstra, and J. Oud. 1996. Shoot regeneration in long term callus cultures derived from mature flowering plants of Cyclamen persicum Mill. Plant Cell Reports 15: 545-548.
• Geier T., H. W. Kohlenbach, and G. Reuther. 1990. Cyclamen. In: P. V. Ammirato, D. A. Evans, W. R. Sharp, and Y. P. S. Bajaj [eds.], Handbook of plant cell culture, vol. 5, Ornamental species, 352-374. McGraw-Hill, New York.
• Gude H., and M. H. G. E. Dijkema. 1997. Somatic embryogenesis in tulip. Proceedings of the Seventh International Symposium on Flower Bulbs, 10-16 March 1996, Herzliya. Israel Acta Horticulturae 430: 275-280.
• Kiviharju E., U. Tuominen, and T. Tormala. 1992. The effect of explant material on somatic embryogenesis of Cyclamen persicum Mill. Plant Cell, Tissue and Organ Culture 28: 187-194.
• Murashige T., and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantamm 15: 473-497.
• Takamura T., I. Miyajima, and E. Matsuo. 1995. Somatic embryogenesis of Cyclamen persicum Mill. ‘Anneke’ from aseptic seedlings. Plant Cell Reports 15: 22-25.
• Wang L., X.-M. Bao, Y.-H. Liu, and S. Hao. 1994. Origin of direct somatic embryos from cultured inflorescence axis segments of Freesia refracta. International Journal Plant Science 155(6): 672-676.
• Winkelmann T., A. Hohe, and H. G. Schwenkel. 1996. Establishing embryogenic longterm suspension cultures in Cyclamen persicum Mill. Plant regeneration via suspension culture systems. Abstracts of the Third Meeting of the Working Group COST 822, 5-8 September 1996, 5. Sanremo.